Flamma® 552 Sulfo-NHS ester
Size of product (mg)
-
1 mg
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5 mg
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25 mg
Flamma® 552 Sulfo-NHS ester
Cat. No. List below
Description
The dye exhibits excellent optical properties and can be efficiently excited using 532, 543, 546, or 555 nm laser lines, making it compatible with a wide range of fluorescence imaging systems. Flamma 552 is particularly well-suited for detecting low-abundance biomolecules due to its high sensitivity and ability to achieve high molar ratios during conjugation.
Key Features:
1. Bright yellow fluorescence with Ex/Em maxima at 550/564 nm
2. High extinction coefficient (≥ 136,000 cm?¹M?¹)
3. Excellent photostability
4. Efficient conjugation to biomolecules
5. Higher water solubility compared to NHS esters
6. Spectrally similar to popular dyes like Alexa555, DyLight549, Cy3, and ATTO550
Applications:
1. Fluorescence microscopy
2. Flow cytometry
3. Immunofluorescence-based assays
4. Cellular labeling and detection
5. Protein and antibody labeling
6. Nucleic acid labeling
7. Labeling of peptides, ligands, and amplification substrates
Advantages:
1. High sensitivity for detecting low-abundance biomolecules
2. Stable fluorescence signal in bioimaging
3. Versatile labeling capabilities for various biomolecules
4. No need for organic co-solvents due to higher water solubility
5. Rapid reaction with amine-modified oligonucleotides and amino groups of proteins
6. Optimized for cellular labeling and detection
7. Membrane-impermeable, making it suitable for cell-surface labeling
The Sulfo-NHS ester reactive group of Flamma 552 readily forms stable amide bonds with primary amines, including the ε-amino groups of lysine residues in proteins and the amine terminus of nucleotides or modified oligonucleotides. This chemistry ensures efficient and durable labeling of target biomolecules.
Flamma® Fluors 552 Sulfo-NHS ester offers researchers a powerful tool for fluorescence labeling, combining high sensitivity, excellent optical properties, and versatile conjugation chemistry in a single molecule. Its higher water solubility compared to NHS esters makes it particularly useful for labeling reactions in aqueous environments, eliminating the need for organic co-solvents and simplifying experimental procedures. The dye's stability and brightness make it ideal for a wide range of bioimaging applications, from single-molecule detection to whole-cell imaging.
- Fluorophore: Flamma® Fluors 552
- Reactive group: Sulfo-NHS ester
- Excitation/Emission Max.(nm): 550/564
- Spectrally similar dyes: Alexa555, DyLight549, Cy3, ATTO550
- Extinction coefficient: ≥ 136,000 cm-1M-1
- CF280: 0.08
- Appearance: Red Solid
- Molecular Weight: 849.23 g/mol
- Solubility: DMF, DMSO
- Storage conditions: -20 ℃, protect from light
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| CWSN1001 | Flamma® 496 Sulfo-NHS ester | 496 | 520 | Alexa488, FITC, Cy2 |
| PWSN1122 | Flamma® 552 Sulfo-NHS ester | 550 | 564 | Alexa555, DyLight549, Cy3, ATTO550 |
| PWSN1415 | Flamma® 581 Sulfo-NHS ester | 581 | 596 | Alexa594, DyLight594 |
| PWSN1215 | Flamma® 648 Sulfo-NHS ester | 648 | 663 | Alexa647, DyLight650, Cy5 |
| PWSN1515 | Flamma® 675Sulfo-NHS ester | 675 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT, CF680 |
| PWSN1301 | Flamma® 749 Sulfo-NHS ester | 749 | 774 | Alexa750, DyLight755, Cy7.5, IRDye750 |
| PWSN1603 | Flamma® 774 Sulfo-NHS ester | 774 | 800 | Cy7.5, DyLight800, IRDye800 |
Background
Flamma® Fluors
BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.
- Covering the full spectral range from UV to NIR
- Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
- High quantum yields and photostability
- High purity and compatible with most of biomolecules
Flamma® Fluors NHS ester / Sulfo-NHS ester
Succinimidyl (NHS) esters are reliable activating moiety for amine labeling due to the formation of the strong amide bond. NHS esters are generally stable when storing in anhydrous and the dark condition at -20. NHS ester dyes display good reactivity toward aliphatic amines yet low reactivity with aromatic amines, alcohols, phenols and histidine. The basicity of an amine is an important factor for its reactivity: virtually all proteins have lysine residues, and most of them have a free N-terminus amine. The reactivity of ε-amino group at lysine is pH dependent that the optimal pH for labeling lysine residue is 8.5~9.5. However, the primary amine at N-terminus usually has a pKa value of ~7, thus it can be selectively conjugated by running the reaction in neutral pH. Due to the solubility issue, Flamma® Fluors NHS esters are required to be dissolved in an organic solvent such as DMSO or DMF and added to aqueous solution. To overcome this limitation, BioActs offers water soluble Flamma® Fluors Sulfo-NHS ester dyes, which can eliminate the usage of organic solvents in the conjugation reaction. NHS and Sulfo-NHS ester dyes are unstable under humid condition, thus they should be in a lyophilized form or a solution in the anhydrous organic solvent such as DMF or DMSO and stored at -20 ℃.
Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma® Fluors
Figure 2. Immunofluorescence imaging and in situ hybridization imaging
Figure 3. Fluorescence images of Flamma® 749 (upper) and Flamma® 774 (bottom) carboxylic acid injected mouse
Citation & Reference
1. Lim, Chang-Keun. Phthalocyanine-aggregated polymeric nanoparticles as tumor-homing near-infrared absorbers for photothermal therapy of cancer. Theranostics 2.9 (2012): 871-879.
2. Park, Jin Woo. Novel cyanine dyes with vinylsulfone group for labeling biomolecules. Bioconjugate chemistry 23.3 (2012): 350-362.
3. Lim, Chang-Keun. Gadolinium-coordinated elastic nanogels for in vivo tumor targeting and imaging. Biomaterials 34.28 (2013): 6846-6852.
4. Keunsoo Jeong. Poly (oxyethylene sugaramide) s: unprecedented multihydroxyl building blocks for tumor-homing nanoassembly. Journal of Materials Chemistry B 1.28 (2013): 3437-3442.
5. Lee, So Jin. TNF-α gene silencing using polymerized siRNA/thiolated glycol chitosan nanoparticles for rheumatoid arthritis. Molecular Therapy 22.2 (2014): 397-408.
6. Yoon, Hong Yeol. Photo-crosslinked hyaluronic acid nanoparticles with improved stability for in vivo tumor-targeted drug delivery. Biomaterials 34.21 (2013): 5273-5280.
7. Park, Solji. Amphiphilized poly (ethyleneimine) nanoparticles: a versatile multi-cargo carrier with enhanced tumor-homing efficiency and biocompatibility. Journal of Materials Chemistry B 3.2 (2015): 198-206.
8. Lee, Eunjung. Co-delivery of chemosensitizing siRNA and an anticancer agent via multiple monocomplexation-induced hydrophobic association. Journal of Controlled Release 210 (2015): 105-114.
9. Xu, Peisheng. Zwitterionic chitosan derivatives for pH-sensitive stealth coating. Biomacromolecules 11.9 (2010): 2352-2358.
10. Ibrahim, Basma M. A strategy to deliver genes to cystic fibrosis lungs: a battle with environment. Journal of controlled release 155.2 (2011): 289-295.
11. Oh, Keun Sang. Accurate sequential detection of primary tumor and metastatic lymphatics using a temperature-induced phase transition nanoparticulate system. International journal of nanomedicine 9 (2014): 2955.
12. Yhee, Ji Young. Tumor-targeting transferrin nanoparticles for systemic polymerized siRNA delivery in tumor-bearing mice. Bioconjugate chemistry 24.11 (2013): 1850-1860.
13. Yoon, Hong Yeol. Glycol chitosan nanoparticles as specialized cancer therapeutic vehicles: Sequential delivery of doxorubicin and Bcl-2 siRNA. Scientific reports 4 (2014).
14. Ryu, Ju Hee. Early diagnosis of arthritis in mice with collagen?induced arthritis, using a fluorogenic matrix metalloproteinase 3–specific polymeric probe. Arthritis & Rheumatism 63.12 (2011): 3824-3832.
15. Hollis, Christin P. In vivo investigation of hybrid paclitaxel nanocrystals with dual fluorescent probes for cancer theranostics. Pharmaceutical research 31.6 (2014): 1450-1459.
16. Koo, Heebeom. The movement of self-assembled amphiphilic polymeric nanoparticles in the vitreous and retina after intravitreal injection. Biomaterials 33.12 (2012): 3485-3493.
17. Zhu, Lei. Real-time monitoring of caspase cascade activation in living cells. Journal of controlled release 163.1 (2012): 55-62.
18. Yoon, Hong Yeol. Bioreducible hyaluronic acid conjugates as siRNA carrier for tumor targeting. Journal of Controlled Release 172.3 (2013): 653-661.
19. Yhee, Ji Young. Cancer-targeted MDR-1 siRNA delivery using self-cross-linked glycol chitosan nanoparticles to overcome drug resistance. Journal of Controlled Release 198 (2015): 1-9.
20. Park, Jin Woo. Wide-Ranged Fluorescent Molecular Weight Size Markers for Electrophoresis. Bulletin of the Korean Chemical Society 34.1 (2013): 29-30.
21. Huang, Xinglu. Multiplex Imaging of an Intracellular Proteolytic Cascade by using a Broad Spectrum Nanoquencher. Angewandte Chemie International Edition 51.7 (2012): 1625-1630.
