GN5K DNA Marker (5 x 500 uL)
Cat. No.: SB-G3361-05
Size: 5 x 500 uL
Description
The GN5K DNA Marker, is ready-to-use and designed for sizing and approximate quantification of wide range double-strand DNA on agarose and polyacrylamide gels. The ladder is composed of nine purified individual DNA fragments (in base pairs): 5000, 3000 , 2000, 1500, 1000, 750, 500, 250 and 100. It contains one reference band (500 bp) for easy quatitative indicator, with concentration of 100 ng/5 μl. The other bands has the approximate concentration of 50 ng / 5 μl .
The DNA marker is ready to use-it is premixed with 6 × DNA loading buffer for direct loading on gel.
Storage and Handling Conditions
Transport use wet ice. Store at 4 ℃ for periods up to 12 months. For longer periods, stored at -20 ℃ .
Assay Protocol
- Mix gently, and then load 1 μl per 1 mm gel lane.
Note:
- Do not heat before loading. Avoid repeated freezing and thawing
- For your safety and health, please wear lab coat and disposable gloves.
GN5K DNA Marker
1 % TAE agarose gel stained with EB ( loading with 5 μL DNA marker )