Western Protein Marker I (Exposure)
Cat. No.: SB-G2086-250UL
Size: 250UL
Description
Western Protein Marker I are a mixture of eight high-purity recombinant proteins and two pre-stained recombinant proteins, which is convenient for monitoring of electrophoresis and transfer efficiency. It is suitable as a protein molecular weight standard for SDS-PAGE and Western Blot.
The bands display in Tris-Glycine gel range from 1 5 - 154 kDa (~1 2 , ~ 22 , ~ 28 , ~3 8 , ~ 50 , ~ 62 , ~7 0 , ~ 78 , ~1 13 , ~ 154 kDa ), of which 7 0 kDa is an orange-red pre-stained band, 1 2 kDa is a blue pre-stained band, and the others can bind almost all types of antibodies (except chicken antibodies). Western Protein Marker I can bind the antibody to the target protein at the same time and develop color by ECL or other methods.
Storage and transportation
Ship with wet ice; Store at -20℃, valid for 12 months.
Product Components
| Component Number | Component | G2086-250UL |
| G2086-1 | Western Protein Marker I ( Exposure ) | 250 μL |
| G2086-2 | Dilution Buffer | 2×1mL |
| Product Manual | 1 copy | |
Assay Protocol / Procedures
- 1. Allow the tube to reach room temperature and thoroughly mix before use.
- 2. For the use of primary antibody as mouse antibody, it is recommended to take this product 5 μL protein electrophoresis was performed simultaneously with the experimental sample; For the rabbit antibody with primary antibody, it is recommended to dilute the produ ct by 4 times with The provided dilution buffer and take 5 μL prot e n electrophoresis was performed simultaneously with the experimental sample.
- 3. Prestained Protein Marker II should be stored at -20℃ immediately after use.
note
- 1. If the Western protein marker I exposed band is thick, it can be used after appropriate dilution with the dilution buffer. After dilution, the color of the two pre- stained protein bands in the marker will weaken correspondingly, which is a normal phenomenon.
- 2. This product cannot be heated, which will cause the protein bands to degrade or discolor.
- 3. Please wear lab coat and disposable gloves during operation.



















