EtB"Out" Nucleic Acid Staining Solution 2.0

Cat. No: YBB-FYD010-200P



size: EtB"Out" Nucleic Acid Staining Solution 2.0 (20,000×)    1 ml :  (200 preps)


Description

EtB"Out" Nucleic Acid Staining Solution aims to replace traditional EtBr (ethidium bromide) in performing nucleic acid detection in agarose gels.
EtBr has long been known as a strong mutagen, however EtB"Out" causes only neglectable mutations in the Ames test.

The sensitivity of EtB"Out" is identical to that of EtBr. Under UV light, EtB"Out" emits green fluorescence when bound to DNA or RNA. EtB"Out" can be excited at 290 nm and 490 nm. The fluorescence emission peak of EtB"Out" when it bound to DNA is at 537 nm.



Features

   *Economic: use only 5 μl in 100 ml of agarose gel

   *Sensitive: sensitivity is comparable and even better than EtBr, DNA concentration as low as 5 ng can be detected

   *Safe: non-mutagenic, non-toxic, non-carcinogenic

   *Green: no hazardous waste


Applications

Nucleic acids detection (dsDNA and ssRNA) within agarose gel after electrophoresis under UV and blue light illumination



Quality Assurance

The quality of the EtB"Out" Nucleic Acid Staining Solution is tested on a lot-to-lot basis. Nucleic acids are extracted from tissues and serial diluted. Electrophoresis is performed and the agarose gel is stained with EtB"Out" Nucleic Acid Staining Solution to ensure the performance.



Excitation and Emission Wavelength




Results


Nucleic staining using EtB"Out" Nucleic Acid Staining Solution.
Two individual agarose gels were prepared with 5 μl of EtBr and EtB"Out" respectively.  Staining results were examined under UV illumination.   Identical results were observed and EtB"Out" even showed a better staining result than using EtBr. 


Specification

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