Hank's Balanced Salt Solution with Ca2+, Mg2+ and 20 mM HEPES without Phenol Red
Cat. No. DCP-HBSSH-R1X
Size: 500 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
Description:
Hank's Balanced Salt Solution (HBSS) with Ca2+ and Mg2+ and HEPES is a widely used physiological buffer in cell culture and biological research. This carefully formulated solution contains a blend of inorganic salts, including sodium chloride, potassium chloride, calcium chloride, magnesium sulfate, magnesium chloride, sodium phosphate, potassium phosphate, and sodium bicarbonate, along with glucose. The presence of calcium and magnesium ions distinguishes this variant from calcium- and magnesium-free HBSS, making it particularly suitable for maintaining cell-to-cell adhesion, supporting cellular signaling processes, and preserving membrane integrity. With a pH of 7.4 at 25°C and an osmolality isotonic with body fluids, HBSS with Ca2+ and Mg2+ effectively maintains pH balance and osmotic equilibrium. This solution combines Hank's Balanced Salt Solution (HBSS) with 20 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid), a buffering agent that helps maintain pH stability in biological systems. This solution is commonly used for cell washing, short-term cell maintenance, transport medium, and as a base for preparing other reagents, making it an essential tool in various cell culture and experimental procedures.
Application:
- Washing Cells: HBSS is commonly used to rinse cells before dissociation, ensuring that any residual media or serum components are removed.
- Transporting Cells or Tissues: The solution serves as a transport medium, preserving cell viability during transportation.
- Diluting Cells for Counting: HBSS provides a suitable environment for diluting cell suspensions, facilitating accurate cell counting and analysis.
- Preparing Reagents: The solution acts as a base for preparing other reagents and solutions used in biological experiments.
Advantages:
- Physiological pH maintenance: HBSS effectively maintains the pH balance of cell cultures, providing a stable environment for cellular functions.
- Osmotic balance: The solution is isotonic with body fluids, helping to maintain proper osmotic balance for cells.
- Essential ion provision: HBSS supplies cells with water and crucial inorganic ions necessary for various cellular processes.
- Versatility: It can be used for multiple purposes, including cell washing, transportation of cells or tissues, cell counting, and reagent preparation.
- Cell adhesion support: The presence of calcium and magnesium ions helps maintain cell-to-cell adhesion, which is crucial for many cell types.
- Membrane integrity preservation: Ca2+ and Mg2+ ions contribute to preserving the integrity of cell membranes.
Composition:
| Ingredients |
Concentration |
| Sodium Chloride |
137.93 mM |
| Potassium Chloride |
5.33 mM |
| Calcium Chloride |
1.26 mM |
| Magnesium Sulfate |
0.41 mM |
| Magnesium Chloride |
0.49 mM |
| Sodium Phosphate Dibasic |
0.338 mM |
| Potassium Phosphate Monobasic |
0.44 mM |
| Sodium Bicarbonate |
4.17 mM |
| D-Glucose (Dextrose) |
5.56 mM |
| HEPES |
20 mM |
Quality Control:
- Appearance: Clear solution.
- pH: 7.4
- Sterility: Filtered 0.1 micron Twice and 0.04 micron once in a sterile environment.
- DNase activity: None was detected after incubation of plasmid DNA, and this product was used for 18 hours at room temperature.
- RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
-
Storage: 4 ℃
-
Shelf life: 2 years
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of other tracking dyes, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
