Hank’s Balanced Salt Solution w/ Phenol red w/o Sodium bicarbonate: 1X
Cat No.: DCP-HBSSR-B1X
Size: 500 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
[+] Phenol Red, [-] Sodium Bicarbonate
Description:
Hank's Balanced Salt Solution with Phenol Red without Sodium Bicarbonate is a precisely formulated physiological salt solution designed to support mammalian cell metabolism in tissue culture applications. This balanced salt solution is derived from the original salt solution described by Sydney Ringer (1885) and has been specifically engineered to provide optimal conditions for maintaining cellular health outside of living organisms. HBSS serves as an essential physiological buffer solution that maintains cell integrity and viability during laboratory procedures.The solution contains phenol red as a pH indicator but requires the addition of sodium bicarbonate prior to use to achieve complete buffering capacity.
Key Functions and Applications:
- Maintains medium within physiological pH range after sodium bicarbonate addition, creating an optimal environment for cellular processes.
- Establishes and preserves intracellular and extracellular osmotic balance, preventing osmotic shock to cells.
- Provides glucose as an energy source for cell metabolism, supporting cellular functions during experimental procedures.
- Facilitates various cell culture applications including washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.
Composition:
| Ingredients |
mg/L |
| INORGANIC SALTS |
|
| Calcium Chloride Anhydrous |
140.00 |
| Disodium Hydrogen Phosphate |
48.000 |
| Magnesium Sulfate Anhydrous |
49.000 |
| Magnesium Chloride |
46.650 |
| Potassium Chloride |
400.000 |
| Potassium Dihydrogen Phosphate Anhydrous |
60.000 |
| Sodium Chloride |
8000.000 |
| OTHERS |
|
| D-Glucose |
1000.000 |
| Phenol Red Sodium Salt |
11.000 |
Quality Control:
- Appearance: Red colored, clear solution.
- pH: 7.4
- Osmolality in mOsm/Kg H2O: 260.00 - 300.00
- Sterility: No bacterial or fungal growth is observed after 14 days of incubation, as per USP specification.
- Toxicity Test: Passes
- Endotoxin Content: NMT 1EU/ml
Storage and Shelf Life:
- Store at 2-8 °C away from bright light.
- Shelf life is 24 months.
- Use before expiry date given on the product label.
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
