Hank’s Balanced Salt Solution w/ Phenol red and Sodium bicarbonate: 1X
Cat No.: DCP-HBSSRB1X
Size: 500 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
[+] Phenol Red, [+] Sodium Bicarbonate
Description:
All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced salt solution (BSS). Hank’s Balanced Salt Solution (HBSS) 1X with Phenol Red and Sodium Bicarbonate is a physiological buffer solution designed to support cell culture applications by maintaining osmotic balance, pH stability, and metabolic activity. Formulated with inorganic salts, glucose, and pH indicators, it serves as a foundational medium for washing cells, tissue dissociation, and short-term cell maintenance.
Applications:
- Cell washing and short-term maintenance
- Preparation of enzymatic dissociation reagents
- Media supplementation for pH buffering
Composition:
| Ingredients |
mg/L |
| INORGANIC SALTS |
|
| Calcium Chloride Anhydrous |
140.00 |
| Disodium Hydrogen Phosphate |
48.000 |
| Magnesium Sulfate Anhydrous |
49.000 |
| Magnesium Chloride |
46.650 |
| Potassium Chloride |
400.000 |
| Potassium Dihydrogen Phosphate Anhydrous |
60.000 |
| Sodium Chloride |
8000.000 |
| Sodium Bicarbonate |
350.000 |
| OTHERS |
|
| D-Glucose |
1000.000 |
| Phenol Red Sodium Salt |
11.000 |
Quality Control:
- Appearance: Red colored, clear solution.
- pH: 7.4
- Osmolality in mOsm/Kg H2O: 265.00 - 305.00
- Sterility: No bacterial or fungal growth is observed after 14 days of incubation, as per USP specification.
- Toxicity Test: Passes
- Endotoxin Content: NMT 1EU/ml
Storage and Shelf Life:
- Store at 2-8 °C away from bright light.
- Shelf life is 24 months.
- Use before expiry date given on the product label.
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
