DynaMarker DNA Small
Product#: FNK-DM100
$204.80
Availability:
Ships in 1-2 Weeks
DynaMarker DNA Small
Cat No: FNK-DM100
Packing Size: 7 μg (50 μl), approximately 50 loadings
Storage condition: -20℃
Storage buffer: 10 mM Tris-HCl (pH 7.5), 100 mM NaCl, 1 mM EDTA
Recommended Loading: 1 µl / lane
Supplied product: 6 × BPB loading dye (EDTA, glycerol and bromphenol blue are contained)
Storage condition: -20℃
Storage buffer: 10 mM Tris-HCl (pH 7.5), 100 mM NaCl, 1 mM EDTA
Recommended Loading: 1 µl / lane
Supplied product: 6 × BPB loading dye (EDTA, glycerol and bromphenol blue are contained)
Description
This product is for research use only.
DynaMarker DNA Small consists of 20, 30, 40, 50, 60, 75 and 100 bp of blunt-end dsDNA. The order and size of each fragment is easy to distinguish and the marker shows very sharp bands. The DNA size marker is ideal for sizing small PCR fragments on non-denaturing acrylamide gel electrophoresis. It is possible to use DynaMarker DNA Small on high-concentration agarose gel electrophoresis but acrylamide gel electrophoresis provides high-resolution separation of small DNA fragments (less than 100 bp).
Electrophoresis of DynaMarker Small DNA
Gel: Non-denaturing acrylamide gel (10%)
Loading: DynaMarker Small DNA, 1 μl
Running buffer: 1 × TBE
Time and voltage: 200V, 20 min After electophoresis, electrophoresed gel was stained with 0.5 μg/ml of EtBr for 15 min.
DynaMarker DNA Small consists of 20, 30, 40, 50, 60, 75 and 100 bp of blunt-end dsDNA. The order and size of each fragment is easy to distinguish and the marker shows very sharp bands. The DNA size marker is ideal for sizing small PCR fragments on non-denaturing acrylamide gel electrophoresis. It is possible to use DynaMarker DNA Small on high-concentration agarose gel electrophoresis but acrylamide gel electrophoresis provides high-resolution separation of small DNA fragments (less than 100 bp).
Electrophoresis of DynaMarker Small DNA
Gel: Non-denaturing acrylamide gel (10%)
Loading: DynaMarker Small DNA, 1 μl
Running buffer: 1 × TBE
Time and voltage: 200V, 20 min After electophoresis, electrophoresed gel was stained with 0.5 μg/ml of EtBr for 15 min.
Ideal for analysis of small PCR fragments
DynaMarker® DNA Small is composed of 20, 30, 40, 50, 60, 75 and 100 bp of dsDNA fragments. It is ideal for sizing small PCR fragments on non-denaturing acrylamide gel electrophoresis.
Designed for superior visibility
Easy recognition of DNA size on UV illuminator.
Supplied with loading dye solution
Just mix 1/6 volume of 6 ? BPB Loading Dye with your DNA sample, then ready to load.
DynaMarker® DNA Small is composed of 20, 30, 40, 50, 60, 75 and 100 bp of dsDNA fragments. It is ideal for sizing small PCR fragments on non-denaturing acrylamide gel electrophoresis.
Designed for superior visibility
Easy recognition of DNA size on UV illuminator.
Supplied with loading dye solution
Just mix 1/6 volume of 6 ? BPB Loading Dye with your DNA sample, then ready to load.
Recommended usage
Small DNA fragments (approximately 20 to 100 bp) are often separated on 10-15% of non-denaturing polyacrylamide gel electrophoresis. For example, DynaMarker DNA Small can be run on 10 % non-denaturing polyacrylamide gel as below.
1. Preparation of 10 % polyacrylamide gel (20 ml gel)
1. Preparation of 10 % polyacrylamide gel (20 ml gel)
40 % acrylamide : bis solution (19:1) 5.0 ml
10 × TBE 2.0 ml
H2O to 20 ml
10 × TBE 2.0 ml
H2O to 20 ml
2. After mixing reagents described above, add 20 µl of TEMED and 160 µl of 10 % ammonium persulfate. Mix quickly and then pour the gel into the mold of a vertical gel apparatus (20 ml is enough gel solution for two 7 cm × 8 cm, thickness 0.1 cm gels). The gel apparatus should be assembled according to the manufacturer’s protocol and ready to run with 1 × TBE buffer.
3. Loading and electrophoresis
Prepare DNA sample for electrophoresis as below.
1) Size Marker:
DynaMarker DNA Small 1 µl
distilled water 4 µl
6 × BPB loading dye 1 µl
2) Sample to examine:
DNA sample 5 µl
6 × BPB loading dye 1 µl
DynaMarker DNA Small 1 µl
distilled water 4 µl
6 × BPB loading dye 1 µl
2) Sample to examine:
DNA sample 5 µl
6 × BPB loading dye 1 µl
Mix the DNA solution with 6 × BPB loading dye in a tube as above. Load the mixture onto a well of 10 % non-denaturing polyacrylamide gel and start electrophoresis. After the tracking dye has migrated an appropriate distance through gel, stop the electrophoresis. To stain with ethidium bromide, disassemble the apparatus and transfer the polyacrylamide gel to a gel tray filled with distilled water containing 0.5 µg/ml ethidium bromide for approximately 15 minutes*. The stained DNA can be visualized using UV transilluminator.
* Longer staining time may decrease the DNA band intensity because small DNA diffuses within the gel over time.
Related products
- DynaMarker DNA Low D (#DM112): for determining the size of low size DNA (50 to 1,000 bp)
- DynaMarker DNA High D (#DM122): for determining the size of high size DNA (300 to 10,000 bp)
- DynaMarker for Plasmid D (#DM132): for determining the size of high size DNA (200 to 7,000 bp)
