Hank's Balanced Salt Solution With Phenol red and Sodium bicarbonate Without Calcium: 1X
FluxMPS™ Hank's Balanced Salt Solution With Phenol red and Sodium bicarbonate Without Calcium: 1X
An MPS-grade, calcium-free Hank's Balanced Salt Solution (HBSS) 1X formulated with magnesium, sodium bicarbonate, and phenol red for real-time pH monitoring. Quadruple-stage filtered to microchannel-safe purity using ultrapure Type 1 water, this calcium-free buffer prevents interference with enzymatic dissociation protocols such as trypsinization while retaining magnesium for enzymatic cofactor and ATPase-dependent functions.
- Filtered 0.1 µm membrane twice and 0.04 µm membrane twice for ultra-clean, microchannel-safe purity
- Endotoxin content not more than (NMT) 1 EU/mL
- Calcium-free formulation prevents interference with calcium-sensitive enzymatic reactions like trypsinization
- Magnesium retained to support ATPase activity and other magnesium-dependent enzymatic processes
- Phenol red included for real-time visual assessment of medium pH
- Sodium bicarbonate buffering supports short-term cell transport in ambient CO2 environments
- Manufactured with ultrapure Type 1 water (18.2 MΩ·cm) under ISO 13485-certified, CE-approved facilities
- Custom pH, concentrations, additives, and modifications available on request
- pH7.4
- Osmolality270.00 - 310.00 mOsm/kg H2O
- CalciumNot present (calcium-free)
- MagnesiumRetained
- EndotoxinNMT 1 EU/mL
- SterilityNo growth after 14 days (USP)
- Filtration0.1 µm x2 + 0.04 µm x2
- Storage2-8°C, away from light
- Shelf Life24 months
- AppearanceRed colored, clear solution
Engineered where standard buffers fail
Conventional 0.22 µm-filtered balanced salt solutions can carry subvisible particulates, uncontrolled ion content, and residual calcium that interferes with enzymatic dissociation. FluxMPS™ balanced salt solutions are built around a defined, calcium-free ionic profile and multi-stage filtration to protect microchannel geometries and sensitive downstream assays.
Microchannel-safe purity
Sequential 0.1 µm and 0.04 µm membrane filtration, each applied twice, minimizes particulate load relative to single-pass 0.22 µm filtration commonly used in conventional balanced salt solutions.
Calcium-free, magnesium-retained design
The absence of calcium prevents interference with calcium-sensitive enzymatic reactions such as trypsinization, while retained magnesium continues to support ATPase activity and other magnesium-dependent cellular processes.
Ultrapure-grade water
Formulated with Type 1 ultrapure water (18.2 MΩ·cm), aligned with USP <85> water-quality expectations for sensitive cell and molecular biology work.
Real-time pH monitoring
Phenol red enables real-time visual assessment of medium pH during experiments, while sodium bicarbonate provides buffering capacity suitable for short-term cell transport in ambient CO2 environments.
Defined, traceable composition
Every inorganic salt, D-glucose, and phenol red sodium salt component is lot-released to a fixed mg/L specification, giving reproducible ionic conditions batch to batch.
Customization on demand
Alternate concentrations, additional chemicals, compounds, proteins, supplements, and different pH values can be formulated on inquiry.
Quadruple-stage filtration system
This buffer is sterile-filtered through a 0.1 µm membrane twice and a 0.04 µm membrane twice — four sequential filtration passes designed to protect enzymatic reagents, microfluidic channels, and downstream assays from particulate and bioburden carry-over.
-
1
0.1 µm Pre-filtration I
Removes large particulates and aggregates from the bulk solution, extending the service life of downstream filters.
-
2
0.04 µm Pre-filtration II
Retains fine particulates and reduces bioburden well below the size of the smallest mycoplasma types, which can be about 0.2 micron.
-
3
0.1 µm Sterile-filtration I
A second 0.1 µm pass provides redundancy ahead of final sterile filtration.
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4
0.04 µm Sterile-filtration II — Final Polish
Final polishing pass performed under ISO Class 5 (Class 100) conditions ahead of aseptic fill, helping prevent mycoplasma contamination.
Performance vs. conventional buffer
Sequential 0.1 µm and 0.04 µm filtration, each applied twice, removes finer particulates than the single 0.22 µm pass used in conventional balanced salt solutions and helps guard against mycoplasma-scale contaminants.
© Diagnocine® — DCP-HBSSRB-C1X
Where calcium-free HBSS fits your workflow
This calcium-free, magnesium-retained HBSS with phenol red and sodium bicarbonate supports cell washing and resuspension in calcium-sensitive protocols (such as enzymatic dissociation where calcium inhibits trypsin activity), magnesium-dependent enzymatic and ion-channel studies, real-time pH monitoring via phenol red, and short-term cell transport in ambient CO2 environments thanks to bicarbonate buffering.
Automated Bioreactors & Robotics
For automated bioreactor and robotic liquid-handling platforms where valve and sensor fouling drive downtime, an optional 0.01 µm (10 nm) ultra-filtered variant of this buffer can be requested.
- Total Particulate Exclusion: finer filtration reduces particulate load beyond the standard quadruple-stage architecture
- Valve & Sensor Protection: minimizes fouling risk in automated dispensing hardware
- Extended Perfusion Stability: supports longer unattended perfusion runs
Inquiry Required: the 0.01 µm (10 nm) ultra-filtered grade is available on request — contact support@diagnocine.com.
Micro Physiological System (MPS) & Chip
Ultrapure, calcium-free perfusion buffer for chip-based culture platforms requiring defined ionic conditions.
Wash, Dilution & Reconstitution
Preferred for calcium-sensitive protocols such as enzymatic dissociation, where residual calcium can inhibit trypsin activity during cell washing and resuspension.
iPSC-Derived Model Handling
Calcium-free washing conditions support gentle handling of iPSC-derived models during dissociation and reseeding steps.
Endothelial & Primary Cell Perfusion
Magnesium-dependent processes, including ATPase activity and ion channel research, are supported in the absence of calcium interference.
ELISA, Blotting & Blocking
Low-particulate, defined-ionic-strength buffer suitable as a wash or dilution base for immunoassay workflows.
Microscopy & Optical Sensing
Built-in phenol red provides a visual pH readout during live-cell imaging and short-term transport between imaging sessions.
Full specification sheet
All values below are specific to DCP-HBSSRB-C1X as tested and released by Diagnocine.
| Parameter | Specification |
|---|---|
| Formulation | Calcium-free HBSS with Magnesium, Sodium Bicarbonate, and Phenol Red |
| Appearance | Red colored, clear solution |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | 270.00 - 310.00 mOsm/kg H2O |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> | NMT 1 EU/mL |
| Sterility USP <71> | No bacterial or fungal growth after 14 days of incubation |
| Toxicity Test | Passes |
| Particulate Matter USP <788> | Compliant, Method 2 |
| Water Quality | Ultrapure Type 1 water, 18.2 MΩ·cm |
| Fill Environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage Temperature | 2-8°C, away from bright light |
| Shelf Life | 24 months |
| Use Before | Expiry date given on product label |
| Parameter | Specification |
|---|---|
| Manufacturing QMS | ISO 13485:2016 certified facility (Suppliers of DiagnoCine Precision) |
| Regulatory Alignment | CE-approved manufacturing; final packaging, QA and testing at DiagnoCine R&D and Quality Testing Center |
| Production & Customization Site | DiagnoCine Precision, Totowa, New Jersey, USA |
| Intended Use | Research Use Only (RUO) |
Full composition
Composition is expressed in mg/L exactly as released per lot, grouped under the original formulation categories.
| Component | CAS Number | Concentration (mg/L) |
|---|---|---|
| Disodium Hydrogen Phosphate (Na2HPO4) | 7558-79-4 | 48.000 |
| Magnesium Sulfate Anhydrous (MgSO4) | 7487-88-9 | 49.000 |
| Magnesium Chloride (MgCl2) | 7786-30-3 | 46.650 |
| Potassium Chloride (KCl) | 7447-40-7 | 400.000 |
| Potassium Dihydrogen Phosphate Anhydrous (KH2PO4) | 7778-77-0 | 60.000 |
| Sodium Chloride (NaCl) | 7647-14-5 | 8000.000 |
| Sodium Bicarbonate (NaHCO3) | 144-55-8 | 350.000 |
| Component | CAS Number | Concentration (mg/L) |
|---|---|---|
| D-Glucose | 50-99-7 | 1000.000 |
| Phenol Red Sodium Salt | 34487-61-1 | 11.000 |
Manufacturing & compliance
DCP-HBSSRB-C1X is manufactured under ISO 13485-certified and CE-approved facilities, with final packaging, quality assurance, and testing performed at the DiagnoCine R&D and Quality Testing Center.
ISO 13485:2016 QMS
Manufactured under ISO 13485-certified, CE-approved facilities (Suppliers of DiagnoCine Precision).
Ultrapure Type 1 Water
Formulated with Type 1 ultrapure water, 18.2 MΩ·cm resistivity.
ISO Class 5 Fill & Finish
Final sterile filtration and aseptic fill under ISO Class 5 (Class 100) conditions.
Micro-Batch Precision
Custom pH, concentration, and additive modifications assembled at DiagnoCine Precision, Totowa, New Jersey, USA.
Endotoxin USP <85>
Bacterial Endotoxins Test: NMT 1 EU/mL.
Particulate USP <788>
Tested per USP <788> Method 2 particulate matter requirements.
Osmolality USP <785>
270.00 - 310.00 mOsm/kg H2O per lot.
Documentation / CoA
Certificate of Analysis available per lot upon request.
How DCP-HBSSRB-C1X compares
A comparison of purity architecture and formulation traits against conventional and standard alternative balanced salt solutions.
| Parameter | DCP-HBSSRB-C1X (FluxMPS™) | Conventional Balanced Salt Solution | Standard Alternative |
|---|---|---|---|
| Calcium-Free, Magnesium-Retained Formulation | check_circle | cancel | cancel |
| Final Filtration Pore Size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of Filtration Stages | 4 (0.1 µm x2 + 0.04 µm x2) | 1 | 1 |
| Endotoxin Specification | NMT 1 EU/mL | Not specified | Not specified |
| USP <788> Particulate Compliance | check_circle | cancel | cancel |
| Water Quality | Ultrapure Type 1 (18.2 MΩ·cm) | Not specified | Purified water |
| Manufacturing QMS | ISO 13485:2016 | Not specified | Not specified |
| Microfluidic Channel Compatibility | check_circle | cancel | cancel |
| pH / Osmolality Reproducibility | 7.4 / 270-310 mOsm/kg H2O per lot | Variable | Variable |
| Custom Formulation Available | check_circle | cancel | cancel |
Frequently asked questions
Common questions about DCP-HBSSRB-C1X, its filtration architecture, and formulation.
Supporting literature
Curated literature relevant to balanced salt solutions, calcium-free buffer applications, and microphysiological system workflows.
- Hanks JH, Wallace RE. Relation of oxygen and temperature in the preservation of tissues by refrigeration. Proc Soc Exp Biol Med. 1949. doi:10.3181/00379727-71-17131
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014. doi:10.1038/nbt.2989
- Low LA, et al. Organs-on-chips: into the next decade. Nat Rev Drug Discov. 2021. doi:10.1038/s41573-020-0079-3
- Ingber DE. Human organs-on-chips for disease modeling, drug development and personalized medicine. Nat Rev Genet. 2022. doi:10.1038/s41576-021-00413-z
- Zhang B, Radisic M. Organ-on-a-chip devices advance to market. Lab Chip. 2017. doi:10.1039/C6LC01554A
- Ding JL, Ho B. Endotoxin detection: from Limulus amebocyte lysate to recombinant factor C. Subcell Biochem. 2010. doi:10.1007/978-90-481-9078-2_9
- Freshney RI. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications. Wiley-Blackwell. 2015. doi:10.1002/9781118873686
