Anti-Nob1 (S.cerevisiae) antibody, affinity purified

Product#: 62-211
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Anti-Nob1 (S.cerevisiae) antibody, affinity purified


General Information  

 Cat. No. :62-211
Size :100µl
Host Animal :Rabbit 
Reactivity S.cerevisiae Nob1p. Not tested with other species
Label :Unlabeled 
Product Rabbit polyclonal antibody affinity purified with recombinant Nob1p
Immunogen Recombinant yeast Nob1p expressed in E. coli
Application  
  1. Western blotting (~400 fold dilution)
  2. Immunoprecipitation
  3. Other applications have not been tested.
Storage -20℃
Form Affinity purified IgG in PBS, 1 mg/ml BSA, 0.09 % sodium azide, 50% glycerol
Data Link SGD NOB1/YOR056C 
 

Description 

The 26 S proteasome is a protein complex with a molecular mass of 2,000 kDa. It is essential not only for eliminating damaged or misfolded proteins but also for degrading short lived regulatory proteins involved in cell cycle regulation, DNA repair, signal transduction, apoptosis, and metabolic regulation (1). Nob1p is essential nuclear protein required for biogenesis of the 26S proteasome (2). Nob1p is speculated to serve as a chaperone to join the 20S proteasome with the 19S regulatory particle in the nucleus and to be degraded upon the maturation of the 26S proteasome (3). Nob1p is composed of 459 amino acid residues.
 

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Fig.1 Detection of Nob1p (51.7kD) in the crude extract of S.cerevisiae by Western blotting using this antibody.

 

References 

  1. Tone Y et al “Nob1p, a new essential protein, associates with the 26S proteasome of growing Saccharomyces cerevisiae cells” Gene 243:37-45 (2000) PMID: 10675611
  2. Tone Y and Toh-e A “Nob1p is reqired for biogenesis of the 26S proteasome and degraded upon its maturation in Saccharomyces cerevisiae” Genes & Dev 16 :3142-3157 (2002) PMID: 12502737 

*This product was used in ref. 1 and 2


Aliases for NOB1 Gene

  • NIN1 (RPN12) Binding Protein 1 Homolog 2 3 5
  • NOB1P 2 3 4
  • Phosphorylation Regulatory Protein HP-10 3 4
  • NIN1/PSMD8 Binding Protein 1 Homolog 2 3
  • RNA-Binding Protein NOB1 3 4
  • Nin One Binding Protein 2 3
  • PSMD8 Binding Protein 1 2 3
  • Protein ART-4 3 4
  • PSMD8BP1 3 4
  • MST158 2 3
  • ART-4 2 3
  • NIN1/RPN12 Binding Protein 1 Homolog (S. Cerevisiae) 2
  • Adenocarcinoma Antigen Recognized By T Lymphocytes 4 3
  • EC 3.1.-.- 4
  • MSTP158 3
  • NOB1 5
  • ART4 4
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