Anti-HP1β/CBX1 antibody (rabbit), ChIP grade

Product#: 70-223
$279.00
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Anti-HP1β/CBX1 antibody (rabbit), ChIP grade


General information 

Cat. No. 70-223 
Size 50 ug
Host Species Rabbit 
Reactivity Human and hamster. Expected to react with mouse, chicken, zebra fish, Xenopus and Drosophila orthologs
    due to the sequence identity of the immunogen.
Form  Affinity purified rabbit IgG, 0.44 mg/ml in 0.12 M sodium Fig. 1 Immunofluorescence staining of HP1β in
   Baby Hamster Kidney cells with this antibody. Cells were fixed with para-formaldehyde. The second
   antibody was Alexa Fluor 594- conjugated goat anti-rabbit IgG. phosphate buffer (pH 7.4), 50% glycerol,
   filter- sterilized, azide free
Application
  1. Western blotting (1/2,000~1/10,000) (Fig. 2)
  2. Immunofluorescence staining (Fig1 & Ref. 3)
  3. Chromatin immunoprecipitation (ChIP) (Ref. 3)
Storage -20℃ (long period, -70℃)
Data Link 

UniProtKB/Swiss-Prot P83916 (CBX1_HUMAN)


Description 

Heterochromatin protein 1 (HP1) is a major component of heterochromatin which plays a role in assembly of various proteins on chromatin and gene silencing. The HP1 family is evolutionally conserved, with members in fungi, plants and animals but not prokaryotes, and there are multiple members within the same species. The HP1 family proteins are encoded by a class of genes known as the chromobox (CBX) genes. In humans, HP1β is encoded by the Chromobox homolog 1 (CBX1) gene located on chromosome 17q21.32. HP1β has been observed to interact directly or indirectly with several non-histone proteins with a wide variety of functions (1).

The product is prepared by immunizing rabbit with the synthetic peptide CNEDDDKKDDKN including the C-terminal sequence (176-185) of human HP1β (3) and purified by affinity purification with the peptide.

The antiserum was prepared by the direction of Prof. T. Haraguchi (Ref. 3).

 

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Fig. 1 Immunofluorescence staining of HP1β in Baby Hamster Kidney cells with this antibody. Cells were fixed with para-formaldehyde. The second antibody was Alexa Fluor 594- conjugated goat anti-rabbit IgG.

Fig.2 Identification of HP1β by Western blotting with the antibody in the crude cell extract. Sample: Extract of MCF7 cells The antibody was used at 1,000 fold dilution.


 


References

  1. Lomberk G et al “The Heterochromatin Protein 1 family” Genome Biol 7: 228 Review (2006) PMID: 17224041
  2. Kametaka A et al “Interaction of the chromatin compactioninducing domain (LR domain) of Ki-67 antigen with HP1 proteins” Genes Cells 7: 1231-1242 (2002) PMID: 12485163
  3. Wang F et al “The assembly and maintenance of heterochromatin initiated by transgene repeats are independent of the RNA interference pathway in mammalian cells” Mol Cell Biol 26: 4028-4040 (2006) PMID: 16705157

* This product was used in references 3.
 

Aliases for CBX1 Gene

  • Chromobox 1 2 3 5
  • CBX 2 3 4
  • M31 2 3 4
  • Heterochromatin Protein 1 Homolog Beta 3 4
  • Chromobox Protein Homolog 1 3 4
  • Modifier 1 Protein 3 4
  • HP1Hs-Beta 2 3
  • HP1Hsbeta 3 4
  • HP1-BETA 2 3
  • P25beta 3 4
  • MOD1 2 3
  • Chromobox Homolog 1 (HP1 Beta Homolog Drosophila ) 3
  • Chromobox Homolog 1 (Drosophila HP1 Beta) 2
  • Heterochromatin Protein P25 Beta 3
  • HP1 Beta Homolog (Drosophila ) 2
  • Heterochromatin Protein 1-Beta 3
  • Heterochromatin Protein P25 4
  • Chromobox Homolog 1 2
  • HP1 Beta Homolog 3
  • HP1 Beta 4
  • CBX1 5

 
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