Anti-Nup153 antibody, rat monoclonal (R4C8)
Product#: FNK-70-315
$346.79
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Anti-Nup153 antibody, rat monoclonal (R4C8)
General information
| Cat. No. | ：FNK-70-315 |
| Size | ：200 µg |
| Antigen Species | ：Rat |
| Host Species | ：Rat |
| Immunogen | ：Recombinant GST-fused rat Nup153 (610-1191aa) |
| Isotype | ：Rat IgG2a kappa |
| Epitope | ：610-1191 aa (Zn finger and FG repeats domain) |
| Form | ：Purified monoclonal antibody (IgG) 1mg/ml in PBS, 50% glycerol, filter-sterilized |
| Reactivity | ：Reacts with human, mouse, rat, hamster and monkey |
| Application | ：Western blotting (1/200) ：Immunofluorescent staining (1/50) ：ELISA Other applications have not been tested. ：Additional comments: When injected into the nucleus, R4C8 accumulates into the nuclear pores of Hela cells. R4C8 works in immuno-cytochemistry very well (Fig. 2 & 3). |
| Storage | ：Shipped at 4℃ or -20℃, and upon arrival, aliquot and store at -20℃. |
| Data Link | ：UniProtKB/Swiss-Prot P49790 (NU153_HUMAN), UniProtKBP49791(NU153_RAT) |
Description
The nuclear pore complex (NPC) regulates cargo transport between the cytoplasm and the nucleus. Nup (Nucleoporin) 153 is a large (153kDa) O-linked glycoprotein which is a component of the basket structure located on the nucleoplasmic face of NPC. Nup153 plays a critical role in nuclear export of RNA and proteins. The antibody was purified from the serum-free cultured medium of the hybridoma under mild conditions by propriety chromatography processes.
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Fig.1 Detection of Nup153 by Western blotting with antibody R4C8. Sample is the nuclear membrane fraction of HeLa cells (Left) and NIH3T3 cells (Right). |
Fig.2 Immunofluorescent staining of HeLa cells with antibody R4C8. |
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Fig.3 Immunofluorescent staining of rat neuron with antibody R4C8. The dots are Nup153. |
References (This antibody has been used in the following publications.)
- Iino H et al. Live imaging system for visualizing nuclear pore complex (NPC) formation during interphase in mammalian cells. Genes to CellsVolume 15, Issue 6. IF (hamster)
- Maeshima K et al. Nuclear pore formation but not nuclear growth is governed by cyclin-dependent kinases (Cdks) during interphase. Nature Structural & Molecular Biology volume 17, pages1065–1071 (2010).IF, WB (human)
Aliases for NUP153 Gene
