Anti-Rpn12 (S.cerevisiae) antibody, affinity purified

Product#: 62-209
Ships in 1-2 weeks

Anti-Rpn12 (S.cerevisiae) antibody, affinity purified


DiagnoCine offers excellent  RPN (5, 7, 9, 12) | Affinity Purified Antibodies  from S.cerevisiae to researchers studying Ubiquitination, Proteosome, DNA repair, Signal transduction, Apoptosis, Metabolic Regulation, Damaged & Misfolded Proteins, and Cell Cycle Regulation.
Human diseases include Neurodegenerative Diseases, Cardiovascular Diseases, Inflammatory Responses, Autoimmune Diseases, Systemic DNA Damage responses leading to Alzheimer's Disease, Parkinson's Disease, Pick's Disease, Amyotrophic Lateral Sclerosis (ALS), Huntington's Disease, Creutzfeldt–Jakob Disease, Muscular Dystrophies, and Several Rare forms of Neurodegenerative Diseases associated with Dementia.

RPN antibodies  have  excellent quality and this highly pure antibody can be adapted for Western blotting,  Immunoprecipitation, and other methods after optimization.

General Information  

 Cat. No. :62-209
Size :50µl
Host Animal :Rabbit 
Reactivity S.cerevisiae Rpn12, not tested with other species
Label :Unlabeled 
Product Rabbit polyclonal antibody affinity purified
Immunogen Recombinant yeast Rpn12 expressed in E. coli
  1. Western blotting (1/5,000~1/10,000)
  2. Immunoprecipitation Other applications have not been tested.
Storage Sent at 4℃ or -20℃ and upon arrival, centrifuge down Briefly and store at -20℃
Form Affinity purified IgG in PBS, 1 mg/ml BSA, 0.09 % sodium azide, 50% glycerol
Data Link SGD RPN12/YFR052W 


The 26 S proteasome is a protein complex with a molecular mass of 2,000 kDa. It is essential not only for eliminating damaged or misfolded proteins but also for degrading short-lived regulatory proteins involved in cell cycle regulation, DNA repair, signal transduction, apoptosis, and metabolic regulation (ref.1). The 26S proteasome is composed of the 20S core particle (CP) and the 19S regulatory particle (RP). The RP is further subdivided into lid and base sub-complexes. Rpn12 is one of the non-ATPase subunits of the lid. Rpn12 interacts with an ATPase subunit, Rpt1, of the base. Rpn12, Rpt1 double mutant becomes lethal, suggesting a strong interaction between Rpn12 and Rpt1. In the double mutant cells, the function of the 26S proteasome is completely eliminated.


Fig.1 Detection of Rpn12 (32kD) in the crude extract of S.cerevisiae by Western blotting using this antibody.



  1. Hershko A and Ciechanover A “THE UBIQUITIN SYSTEM.” Annu. Rev. Biochem. 67, 425-479 (1998) PMID: 9759494
  2. Takeuchi J and Toh-e A “Genetic evidence for interaction between components of the yeast 26S proteasome: combination of a mutation in RPN12 (a lid component gene) with mutations in RPT1 (an ATPase gene) causes synthetic lethality.” Mol Gen Genet 262:145-153 (1999) PMID: 10503546
  3. Tone Y et al “Nob1p, a new essential protein, associates with the 26S proteasome of growing saccharomyces cerevisiae cells.” Gene 243: 37-45 (2000) PMID: 10675611
* This antibody has been used in Ref. 2

Aliases for NOB1 Gene

  • NIN1 (RPN12) Binding Protein 1 Homolog 2 3 5
  • NOB1P 2 3 4
  • Phosphorylation Regulatory Protein HP-10 3 4
  • NIN1/PSMD8 Binding Protein 1 Homolog 2 3
  • RNA-Binding Protein NOB1 3 4
  • Nin One Binding Protein 2 3
  • PSMD8 Binding Protein 1 2 3
  • Protein ART-4 3 4
  • PSMD8BP1 3 4
  • MST158 2 3
  • ART-4 2 3
  • NIN1/RPN12 Binding Protein 1 Homolog (S. Cerevisiae) 2
  • Adenocarcinoma Antigen Recognized By T Lymphocytes 4 3
  • EC 3.1.-.- 4
  • MSTP158 3
  • NOB1 5
  • ART4 4

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