Solution in Dulbecco’s Phosphate-Buffered Saline (modified) containing 0.03% ProClin.
Quanitity:
50µg
Concentration:
0.9 - 1.1 mg/mL
Determined by extinction coefficient, absorbance at 280nm of 1.38 equals 1.0 mg of IgG (E1%1cm =13.8).
Purification:
Affinity chromatography using protein G.
Application:
Competitive immunoassay for detection of heptachlor.
Subclass:
IgG1
Storage:
Store at -20°C or -75°C. Avoid freeze-thaw cycles.
Stability:
Frozen, 1 year at -75°C or less.
Note:
For research use only.
Typical standard curve for heptachlor with indirect competitive ELIA (ic-ELISA)
Cross-reactivity
Chlorinated cyclodienes
Cross-reactivity (%)
dieldrin
41
heptachlor
100
heptachlor-cis-epoxide
20
heptachlor-trans-epoxide
7
aldrin
0.8
endrin
2
alpha-endosulfan
0.6
beta-endosulfan
2
endosulfan-sulfate
Pr
cis-chlordane
Pr
trans-chlordane
Pr
% of cross-reactivity was calculated at the 50 % binding point. Pr.: 30% or lower inhibition by up to 200 ng/mL (40 ng/mL in assay) of chlorinated cyclodienes.