Anti-Rpn9 (S.cerevisiae) antibody, purified

Product#: 62-207
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Anti-Rpn9 (S.cerevisiae) antibody, purified


DiagnoCine offers excellent  RPN (5, 7, 9, 12) | Affinity Purified Antibodies  from S.cerevisiae to researchers studying Ubiquitination, Proteosome, DNA repair, Signal transduction, Apoptosis, Metabolic Regulation, Damaged & Misfolded Proteins, and Cell Cycle Regulation.
 
Human diseases include Neurodegenerative Diseases, Cardiovascular Diseases, Inflammatory Responses, Autoimmune Diseases, Systemic DNA Damage responses leading to Alzheimer's Disease, Parkinson's Disease, Pick's Disease, Amyotrophic Lateral Sclerosis (ALS), Huntington's Disease, Creutzfeldt–Jakob Disease, Muscular Dystrophies, and Several Rare forms of Neurodegenerative Diseases associated with Dementia.

RPN antibodies  have  excellent quality and this highly pure antibody can be adapted for Western blotting,  Immunoprecipitation, and other methods after optimization.


General Information  

 Cat. No. :62-207
Size :100µl
Host Animal :Rabbit 
Reactivity S.cerevisiae Rpn9, not tested with other species
Label :Unlabeled 
Product IgG fraction of rabbit polyclonal antibody against Rpn9,. GST antibody was removed by passing through
    GST affinity column.
Immunogen :GST-full length Rpn9 fusion protein expressed in E. coli (ref.2)
Application  
  1. Western blotting (1/1,000~1/2,000)
  2. Immunoprecipitation
Storage Sent at 4℃ or -20℃ and upon arrival, centrifuge down Briefly and store at -20℃
Form Purified IgG in PBS, 1 mg/ml BSA, 0.09% sodium azide, 50% glycerol
Data Link SGD RPN9/YDR427W 
 

Description 

The 26 S proteasome is a protein complex with a molecular mass of 2,000 kDa. It is essential not only for eliminating damaged or misfolded proteins but also for degrading short-lived regulatory proteins involved in cell cycle regulation, DNA repair, signal transduction, apoptosis, and metabolic regulation (ref.1). The 26S proteasome is composed of the 20S core particle (CP) and the 19S regulatory particle (RP). The RP is further subdivided into lid and base sub-complexes. Rpn9 is one of the non-ATPase subunits of the lid. Rpn9 plays a key role in facilitating the assembly of the 26S proteasome or in stabilizing the structure of the 26S proteasome. Rpn9 null mutant is temperature sensitive and exhibits cell cycle and proteasome assembly defects.
 

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Fig.1 Detection of Rpn9 (46kD) in the crude extract of S.cerevisiae by Western blotting using this antibody

 

References 

  1. Takeuchi J et al “Rpn9 is required for efficient assembly of the yeast 26S proteasome.” Mol Cell Biol 19:6575-6584 (1999) PMID: 10490597 WB
* This antibody has been used in the above publication


Aliases for PSMD13 Gene

  • Proteasome 26S Subunit, Non-ATPase 13 2 3 5
  • Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 13 2 3
  • 26S Proteasome Non-ATPase Regulatory Subunit 13 3 4
  • 26S Proteasome Regulatory Subunit P40.5 3 4
  • 26S Proteasome Regulatory Subunit RPN9 3 4
  • 26S Proteasome Regulatory Subunit S11 3 4
  • P40.5 2 3
  • Rpn9 2 3
  • 26S Proteasome Subunit P40.5 3
  • HSPC027 3
  • PSMD13 5
  • S11 3
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