Anti-HP1α/CBX5 antibody (rabbit), affinity purified

Product#: 70-221
$279.00
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Anti-HP1α/CBX5 antibody (rabbit), affinity purified


General information 

Cat. No. 70-221
Size 50 ug
Host Species Rabbit 
Reactivity Human. Not tested with other species
Form  Affinity purified IgG 0.37 mg/ml in 0.12 M sodium phosphate buffer (pH 7.4), 50% glycerol, sterilized by
   filtration, azide free
Application
  1. Western blotting (1/1,000~1/3,000) (Fig.1)
  2. Immunofluorescence staining (1/200~ 1/1,000)
Storage -20℃ (long period, -70℃)
Data Link 

UniProtKB/Swiss-Prot P45973 (CBX5_HUMAN)



Description 

Heterochromatin protein 1 (HP1) is a major component of heterochromatin and plays a role in assembly of various proteins on chromatin and gene silencing. The HP1 family is evolutionally conserved, with members in fungi, plants and animals but not prokaryotes, and there are multiple members within the same species. The HP1 family proteins are encoded by a class of genes known as the chromobox (CBX) genes. In humans, HP1α is encoded by the Chromobox homolog 5 (CBX5) gene located on chromosome 12q13.13. HP1α has been observed to interact directly or indirectly with several non-histone proteins with a wide variety of functions (1).

The product is prepared by immunizing rabbit with the synthetic peptide CEDAENKEKETAKS corresponding to the amino acid sequence 179-191 of human HP1α and purified by affinity purification with the peptide. The antiserum was prepared by the direction of Prof. T. Haraguchi.

70-221_e.png 
Fig. Detection of HP1α protein by Western blotting. Lane 1: Crude extract of MCF7 cells The antiserum was diluted 1,000 fold before use.

 

References

  1. Lomberk G et al “The Heterochromatin Protein 1 family” Genome Biol 7: 228 Review (2006) PMID: 17224041
  2. Kametaka A et al “Interaction of the chromatin compactioninducing domain (LR domain) of Ki-67 antigen with HP1 proteins” Genes Cells 7: 1231-1242 (2002) PMID: 12485163
  3. Wang F et al “The assembly and maintenance of heterochromatin initiated by transgene repeats are independent of the RNA interference pathway in mammalian cells” Mol Cell Biol 26: 4028-4040 (2006) PMID: 16705157

 

Aliases for CBX5 Gene

  • Chromobox 5 2 3 5
  • Chromobox Homolog 5 (HP1 Alpha Homolog, Drosophila) 2 3
  • Heterochromatin Protein 1 Homolog Alpha 3 4
  • Chromobox Protein Homolog 5 3 4
  • Antigen P25 3 4
  • HP1-ALPHA 2 3
  • HP1A 3 4
  • HP1 2 3
  • Chromobox Homolog 5 (Drosophila HP1 Alpha) 2
  • Heterochromatin Protein 1-Alpha 3
  • HP1 Alpha Homolog (Drosophila) 2
  • Epididymis Luminal Protein 25 3
  • Chromobox Homolog 5 2
  • HP1 Alpha Homolog 3
  • HP1Hs Alpha 3
  • HP1Hs-Alpha 2
  • HP1 Alpha 4
  • HEL25 3
  • CBX5 5

 
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