Anti-Rpn5 (S.cerevisiae) antibody, affinity purified

Product#: 62-203
$400.00
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Anti-Rpn5 (S.cerevisiae) antibody, affinity purified
 

DiagnoCine offers excellent  RPN (5, 7, 9, 12) | Affinity Purified Antibodies  from S.cerevisiae to researchers studying Ubiquitination, Proteosome, DNA repair, Signal transduction, Apoptosis, Metabolic Regulation, Damaged & Misfolded Proteins, and Cell Cycle Regulation.
 
Human diseases include Neurodegenerative Diseases, Cardiovascular Diseases, Inflammatory Responses, Autoimmune Diseases, Systemic DNA Damage responses leading to Alzheimer's Disease, Parkinson's Disease, Pick's Disease, Amyotrophic Lateral Sclerosis (ALS), Huntington's Disease, Creutzfeldt–Jakob Disease, Muscular Dystrophies, and Several Rare forms of Neurodegenerative Diseases associated with Dementia.

RPN antibodies  have  excellent quality and this highly pure antibody can be adapted for Western blotting,  Immunoprecipitation, and other methods after optimization.


General Information  

 Cat. No. :62-203
Size :100µl
Host Animal :Rabbit 
Reactivity S.cerevisiae Rpn5p. Not tested with other species
Label :Unlabeled 
Product :Rabbit polyclonal antibody affinity purified with recombinant Rpn5p
Immunogen :Recombinant yeast Rpn5p expressed in E. coli
Application  
  1. Western blotting (~1000 fold dilution)
  2. Immunoprecipitation
  3. Not tested for other applications
Storage :-20℃. To avoid repeated freezing and thawing, store the antibody in aliquots
Form :Purified IgG in 100 mM NaCl, 10 mM Tris-HCl pH 7.4, 0.05 % sodium azide
Data Link SGD RPN5/YDL147W 
 

Description 

The 26 S proteasome is a protein complex with a molecular mass of 2000 kDa. It is essential not only for eliminating damaged or misfolded proteins but also for degrading short-lived regulatory proteins involved in cell cycle regulation, DNA repair, signal transduction, apoptosis, and metabolic regulation (1). Rpn5p is an essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7 (2, 3,). It consists of 445 amino acids with a molecular weight of 51,768.
 

    62-203_e.jpg

Fig.1 Detection of Rpn5 (52kDa) in the crude extract of S. cerevisiae by Western blotting using this antibody

 

References 

  1. Hershko A and Ciechanover A “THE UBIQUITIN SYSTEM” Annu Rev Biochem 67: 425-479 (1998) PMID: 9759494
  2. Finley D et al “Unified nomenclature for subunits of the Saccharomyces cerevisiae proteasome regulatory particle” Trends Biochem Sci 23: 244-245 (1998) PMID 9697412
  3. Isono E et al “The assembly pathway of the 19S regulatory particle of the yeast 26S proteasome” Mol Biol Cell 18: 569-576 (2007) PMID: 17135287
* This product was used in ref. 3


Aliases for PSMD12 Gene

  • Proteasome 26S Subunit, Non-ATPase 12 2 3 5
  • Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 12 2 3
  • 26S Proteasome Non-ATPase Regulatory Subunit 12 3 4
  • 26S Proteasome Regulatory Subunit RPN5 3 4
  • 26S Proteasome Regulatory Subunit P55 3 4
  • Rpn5 2 3
  • P55 2 3
  • PSMD12 5
  • STISS 3
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