Anti-HP1γ/CBX3 antibody (rabbit), ChIP grade

Product#: 70-225
$279.00
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Anti-HP1γ/CBX3 antibody (rabbit), ChIP grade


General information 

Cat. No. 70-225 
Size 50 ug
Host Species Rabbit 
Reactivity Human and hamster. Expected to react with chicken, Xenopus, Drosophila, and zebra fish orthologs
    due to the sequence identity of the immunogen.
Form  Affinity purified IgG, 0.75 mg/ml in 0.12 M sodium phosphate buffer (pH 7.4), 50% glycerol, filter-sterilized,
   Cells were fixed with para-formaldehyde. The second antibody was Alexa Fluor 594-conjugated goat
   anti-rabbit IgG antibody.
Application
  1. Western blotting (1/2,000~1/10,000) (Fig. 2 & Ref. 2)
  2. Immunofluorescence staining (Ref. 2 & 3)
  3. Chromatin immunoprecipitation (ChIP) (Ref. 3)
Storage -20℃ (long period, -70℃)
Data Link 

UniProtKB/Swiss-Prot Q13185 (CBX3_HUMAN)



Description 

Heterochromatin protein 1 (HP1) is a major component of heterochromatin which plays a role in assembly of various proteins on chromatin and gene silencing. The HP1 family is evolutionally conserved, with members in fungi, plants and animals but not prokaryotes, and there are multiple members within the same species. The HP1 family proteins are encoded by a class of genes known as the chromobox (CBX) genes. In humans, HP1γ is encoded by the Chromobox homolog 3 (CBX3) gene. HP1γ has been observed to interact directly or indirectly with several non-histone proteins with a wide variety of functions (Ref 1).

The product is prepared by immunizing rabbit with the synthetic peptide WHSCPEDEAQ-C corresponding to the C-terminal sequence of human HP1γ (Ref 2, 3) and purified by affinity purification with the peptide. The antiserum preparation has been directed by Prof. T. Haraguchi.

 

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Fig. 1 Immunofluorescent staining of HP1γ in Baby Hamster Kidney cells with this antibody. Cells were fixed with para-formaldehyde. The second antibody was Alexa Fluor 594-conjugated goat anti-rabbit IgG antibody.
Fig. 2 Identification of HP1γ in crude cell extract by Western blotting with this bantibody. Sample: Baby Hamster Kidney cells


References

  1. Lomberk G et al “The Heterochromatin Protein 1 family” Genome Biol 7: 228 Review (2006) PMID: 17224041
  2. Kametaka A et al “Interaction of the chromatin compactioninducing domain (LR domain) of Ki-67 antigen with HP1 proteins” Genes Cells 7: 1231-1242 (2002) PMID: 12485163
  3. Wang F et al “The assembly and maintenance of heterochromatin initiated by transgene repeats are independent of the RNA interference pathway in mammalian cells” Mol Cell Biol 26: 4028-4040 (2006) PMID: 16705157

* This product was used in references 2 and 3.
 

Aliases for CBX3 Gene

  • Chromobox 3 2 3 5
  • Heterochromatin Protein 1 Homolog Gamma 3 4
  • Chromobox Protein Homolog 3 3 4
  • Modifier 2 Protein 3 4
  • HP1Hs-Gamma 2 3
  • HECH 3 4
  • Chromobox Homolog 3 (HP1 Gamma Homolog, Drosophila) 3
  • Chromobox Homolog 3 (Drosophila HP1 Gamma) 2
  • Heterochromatin Protein HP1 Gamma 3
  • HP1 Gamma Homolog (Drosophila) 2
  • Heterochromatin-Like Protein 1 3
  • Chromobox Homolog 3 2
  • HP1 Gamma Homolog 3
  • HP1-GAMMA 3
  • HP1 Gamma 4
  • CBX3 5

 
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