Tyrode's Balanced Salt Solution w/ HEPES: 1X
Cat No.: DCP-TBSSH1X
Size: 500 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
[+] HEPES
Description:
Tyrode's Balanced Salt Solution with HEPES is an advanced physiological buffer formulation specifically designed to maintain optimal cellular environments for in vitro research applications. This isotonic solution combines the classical Tyrode's salt composition with HEPES buffering capacity, providing superior pH stability and physiological support for sensitive biological systems. Tyrode's Solution with HEPES represents an enhancement to traditional balanced salt solutions by incorporating HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) buffer, which significantly improves pH stability across various experimental conditions. This solution functions as a multifunctional medium that regulates pH, maintains proper osmotic pressure, and provides essential nutrients for cellular metabolism and survival in vitro.
Applications:
- Cell and Tissue Culture: The balanced formulation provides essential nutrients and maintains optimal pH and osmotic conditions for various cell types during culture and experimental manipulations.
- Tissue Preservation and Transport: The solution creates an ideal environment for temporary preservation and transport of isolated tissues, maintaining viability and physiological function during experimental transitions
- Perfusion Studies: Tyrode's solution is frequently employed for perfusion experiments in mammalian tissues, providing physiological support during extended perfusion protocols
- Isolated Muscle Experiments: The formulation is especially suitable for isolated intestinal and cardiac muscle experiments, maintaining normal physiological function of the tissue while allowing for controlled experimental interventions
Advantages:
- Superior pH Stability: The inclusion of HEPES buffer significantly enhances the solution's buffering capacity compared to conventional Tyrode's formulations, maintaining stable pH (7.2-7.4) even when exposed to ambient CO₂ levels
- Physiological Compatibility: Precisely formulated to simulate physiological conditions, Tyrode's Solution with HEPES provides cells with an environment that closely mimics in vivo conditions
Composition:
| Ingredients |
mg/L |
| INORGANIC SALTS |
|
| Calcium chloride dihydrate |
265.000 |
| Magnesium chloride anhydrous |
46.900 |
| Potassium chloride |
200.000 |
| Sodium bicarbonate |
1000.000 |
| Sodium chloride |
8000.000 |
| Sodium dihydrogen phosphate anhydrous |
50.000 |
| OTHERS |
|
| D-Glucose |
1000.000 |
| HEPES |
2383.000 |
Quality Control:
- Appearance: Colorless, clear solution
- pH: 7.4
- Sterility: No bacterial or fungal growth is observed after 14 days of incubation, as per USP specification.
- Toxicity Test: Passes
Storage and Shelf Life:
- Store at 4 °C away from bright light.
- Shelf life is 24 months.
- Use before expiry date given on the product label.
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
