Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
World’s cleanest buffer for all cell and molecular biology experiments
Description:
Phosphate-Buffered Saline (PBS) with 0.05% Tween-20 (PBST) is a widely used buffer in ELISA and immunostaining procedures due to its effective properties in reducing non-specific binding and enhancing washing efficiency. Tween 20 acts as a non-ionic detergent-based blocking agent, preventing the non-specific binding of antibodies or proteins to surfaces like microplate wells or membranes. This effect reduces background noise and increases the signal of interest. This reduces background noise and improves signal detection in ELISA and Western blotting assays. It is a non-ionic detergent that helps to disrupt non-specific protein-protein interactions during washing steps, enhancing the removal of unbound proteins or antibodies. It also reduces surface tension, allowing for better penetration and removal of unbound molecules.
Applications:
Washing Buffer: PBST is commonly used as a washing buffer in ELISA assays. The addition of Tween-20, a non-ionic detergent, helps to remove non-specifically bound proteins from the microplate wells, thereby reducing background noise and improving the specificity of the assay results.
PBST is used in conjunction with other blocking agents like BSA to block unsaturated binding sites on the microplate surface, further minimizing non-specific interactions.
In immunohistochemistry and other immunostaining techniques, PBST serves as an effective washing solution that helps to remove unbound antibodies, thus preventing non-specific staining and enhancing signal clarity.
Advantages:
Reduction of Non-Specific Binding: The addition of Tween-20, a non-ionic detergent, helps in minimizing non-specific interactions by effectively washing away proteins that are loosely bound to the microplate wells. This leads to clearer and more specific assay results
Improved Signal-to-Noise Ratio: By reducing background noise, PBST enhances the contrast between positive and negative signals, which is crucial for accurate quantification in ELISA assays.
Effective Washing Solution: In immunostaining protocols, PBST is used to wash away unbound antibodies, reducing background staining and enhancing the specificity of antigen-antibody interactions
Maintains Antigen Integrity: The gentle action of Tween-20 helps preserve the structural integrity of antigens during washing steps, which is important for maintaining accurate staining patterns.
Versatility: PBST is suitable for use with both paraformaldehyde-fixed samples and other types of tissue preparations, making it a versatile choice for various immunostaining applications
Composition:
Ingredients
Concentration
NaCl
137 mM
KCl
2.7 mM
Na2HPO4
10 mM
KH2PO4
1.8 mM
Tween 20
0.05%
Quality Control:
Appearance: Clear, Colorless Liquid.
pH: 7.4
Sterility: Filtered 0.1 micron Twice + 0.04 micron in a sterile environment.
DNase activity: None was detected after incubation of plasmid DNA and this product for 18 hr at room temperature.
RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
Storage: 4 ℃
Shelf life: 2 years
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA
