Sterile; Ultrapure, Filtered 0.1-micron membrane ONCE and autoclave sterilized.
World’s cleanest buffer for all cell and molecular biology experiments
Description
TBS Blocking Buffer with Casein (1%) is a specialized reagent used in immunoassays such as Western blotting, ELISA, and immunohistochemistry. This buffer is designed to block non-specific binding sites on membranes and microplates, thus enhancing the specificity and sensitivity of these assays. It contains 1% casein, a protein derived from milk, known for its effective blocking properties. Casein is often purified and sometimes chemically modified to enhance its efficiency. Casein provides excellent blocking efficiency due to its ability to form a dense layer that minimizes gaps and non-specific interactions. This makes it particularly effective in reducing background noise in assaysTris-Buffered Saline (TBS) provides a stable pH environment, essential for maintaining the integrity of proteins and antibodies during assays. TBS is particularly beneficial in applications involving alkaline phosphatase detection systems, as it avoids phosphate interference. Casein blocking buffers are particularly recommended for applications using biotin-avidin complexes, as they provide lower backgrounds than buffers containing non-fat milk.
Applications
Western Blotting: Used to block non-specific sites on membranes, ensuring that antibodies bind specifically to target proteins.
ELISA (Enzyme-Linked Immunosorbent Assay): Reduces background noise by preventing non-specific interactions between assay components.
Immunohistochemistry (IHC): Prevents non-specific binding of antibodies to tissue sections, improving the clarity and accuracy of staining.
Advantages:
Optimal Concentration: The 1% concentration of casein is considered optimal for most applications, providing a balance between effective blocking and maintaining assay sensitivity.
Reduced Cross-Reactivity: As a single-protein blocking agent, casein reduces the risk of cross-reactions with assay components compared to complex mixtures like serum or milk.
Reproducibility and Low Variability: The use of highly purified and modified casein ensures consistent results, making it suitable for high-precision assays.
Composition:
Ingredients
Concentration
Casein
1%
Tris-base
50 mM
Sodium Chloride
150 mM
Quality Control:
Appearance: Clear, Colorless Liquid.
pH: 7.4
Sterility: Filtered 0.1-micron membrane ONCE and autoclave sterilized.
DNase activity: None was detected after incubation of plasmid DNA and this product for 18 hr at room temperature.
RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
Storage: -20 ℃
Shelf life: 1 year
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
