TBST (Tris-buffered saline, 0.05% Tween 20) [20X]
Cat No.: DCP-TBST20X
Size: 500 mL & 1 L
Sterile, Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
Description
Tris Buffered Saline with Tween 20 (TBS-T) is a widely used buffer in various biochemical and molecular biology applications. Tris buffer provides a stable pH environment, which is crucial for consistent experimental results. Tween 20 minimizes non-specific interactions in immunoassays, such as Western blotting and ELISA, leading to clearer and more specific results. The combination of Tris and Tween 20 ensures effective washing of membranes and microtiter plates, removing unbound proteins and reducing background noise. Tween 20 is a mild detergent that helps maintain the native structure and function of proteins during washing steps. TBS-T is designed to maintain the proper buffering environment, ensuring that antigen-antibody binding reactions are not disrupted. TBS-T is used in various applications, including Western blotting, ELISA, and immunohistochemistry. It can be used with a variety of detection methods, such as colorimetric, chemiluminescent, and fluorescent detection. Using the Tris Tween 20 buffer in immunoassays offers significant advantages in terms of reducing non-specific binding, maintaining stable pH, compatibility with phospho-specific antibodies, and versatility across different applications.
Advantages:
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Buffering Capacity: Tris-HCl provides a stable pH environment, which is crucial for maintaining the integrity of the proteins and antibodies involved in the assay. This stability helps in consistent and reproducible results.
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The inclusion of Tween 20, a non-ionic detergent, helps to reduce non-specific protein-protein interactions without affecting the specific binding of antibodies to their antigens. This results in cleaner blots with reduced background noise.
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It is effective in washing away non-specifically bound proteins, thereby enhancing the specificity of the assay. This is particularly useful in Western blotting, where non-specific binding can obscure the detection of target proteins.
Applications:
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Western Blotting: As a wash buffer to remove non-specifically bound proteins from the membrane, ensuring that only specific antigen-antibody interactions are detected.
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ELISA: To wash microtiter plate wells, removing excess reagents and reducing background noise.
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Immunohistochemistry (IHC): As a wash step to remove nonspecific bindings in tissue samples.
Composition:
| Ingredients |
Concentration |
| Tris-buffered saline |
400mM |
| Tween 20 |
1% |
Quality Control:
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Appearance: Clear, Colorless Liquid.
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pH: 7.4
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Ultrapure: Filtered 0.1 micron Twice and 0.04 micron once in a sterile environment.
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DNase activity: None was detected after incubating plasmid DNA and this product for 18 hr at room temperature.
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RNase activity: None RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA
