PBS Blocking Buffer with Gelatin (1%)
Cat No.: DCP-GPBS1X
Size: 100 ml, 500 mL & 1000 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
Description
PBS Blocking Buffer with Gelatin (1%) is a reagent used in various immunoassays, such as Western blotting and ELISA, to block non-specific binding sites on membranes and microplates. This helps improve the sensitivity and specificity of the assays by reducing background noise. This buffer contains 1% gelatin, which is derived from animal collagen. Gelatin is known for its ability to form a gel-like matrix, effectively covering potential non-specific binding sites. Phosphate Buffered Saline (PBS) provides a stable pH environment, essential for maintaining the integrity of proteins and antibodies during assays. Gelatin is generally less effective at blocking non-specific binding compared to casein and BSA. It is more suitable for blocking protein-protein interactions rather than protein-surface interactions, which can result in higher background noise and decreased sensitivity in some assays. Gelatin does not cross-react with mammalian antibodies, making it a good choice for assays involving mammalian samples.
* Not Suitable for All Membranes: Gelatin blocking buffer may not be suitable for PVDF membranes and is better suited for nitrocellulose, nylon, and charge-modified nylon membranes.
Applications
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Western Blotting: Used to block non-specific sites on membranes, ensuring that antibodies bind specifically to target proteins.
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ELISA (Enzyme-Linked Immunosorbent Assay): Reduces background noise by preventing non-specific interactions between assay components.
Advantages:
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Effective Blocking: The high concentration of gelatin provides a dense blocking layer that minimizes non-specific binding, leading to clearer and more accurate results.
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Compatibility: Gelatin blocking buffers are compatible with various detection systems, including biotin, fluorescein, and DIG detection systems.
Composition:
| Ingredients |
Concentration |
| Gelatin |
1% |
| Sodium Chloride |
137 mM |
| Potassium Chloride |
2.7 mM |
| Sodium Phosphate Dibasic Anhydrous |
10.1 mM |
| Potassium Phosphate Monobasic Anhydrous |
1.76 mM |
Quality Control:
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Appearance: Clear, Colorless Liquid.
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pH: 7.4
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Sterility: Filtered 0.1 micron Twice and 0.04 micron once in a sterile environment.
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DNase activity: None was detected after incubation of plasmid DNA and this product for 18 hr at room temperature.
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RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
