Tris-Buffered Saline (TBS) with (0.05%) Tween-20 [1X]
Cat No.: DCP-TBST1X
Size: 100 mL & 500 mL
Sterile, Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
World’s cleanest buffer for all cell and molecular biology experiments
Description
Tris-Buffered Saline (TBS) with 0.05% Tween-20 (TBS-T) is a widely used buffer in ELISA and immunostaining applications. Its formulation provides specific advantages that enhance the effectiveness and reliability of these assays. Tris buffer provides a stable pH environment, which is crucial for consistent experimental results. Tween 20 minimizes non-specific interactions in immunoassays, such as Western blotting and ELISA, leading to clearer and more specific results. The combination of Tris and Tween 20 ensures effective washing of membranes and microtiter plates, removing unbound proteins and reducing background noise. Tween 20 is a mild detergent that helps maintain the native structure and function of proteins during washing steps. TBS-T is designed to maintain the proper buffering environment, ensuring that antigen-antibody binding reactions are not disrupted. TBS-T is used in various applications, including Western blotting, ELISA, and immunohistochemistry. It can be used with a variety of detection methods, such as colorimetric, chemiluminescent, and fluorescent detection. Using the Tris Tween 20 buffer in immunoassays offers significant advantages in terms of reducing non-specific binding, maintaining stable pH, compatibility with phospho-specific antibodies, and versatility across different applications.
Advantages:
Reduction of Non-Specific Binding: The inclusion of Tween-20 helps minimize non-specific interactions between antibodies and the assay surface, leading to a clearer signal and improved specificity in detecting target antigens.
Enhanced Signal Clarity: By washing away unbound antibodies more effectively than standard buffers, TBS-T improves the signal-to-noise ratio, making it easier to distinguish between positive and negative results.
Effective Washing Solution: In immunostaining protocols, TBS-T serves as a washing buffer that removes excess unbound antibodies, thus reducing background staining and enhancing the clarity of specific signals.
Preservation of Antigen Integrity: The gentle action of Tween-20 maintains the structural integrity of antigens during washing steps, which is crucial for accurate staining patterns.
Versatility Across Applications: TBS-T is suitable for various types of samples, including frozen and paraffin-embedded tissues, making it a flexible choice for different immunostaining techniques.
Applications:
Washing Buffer: TBS-T is commonly used as a washing buffer during ELISA procedures. It effectively removes unbound antibodies and proteins from the wells, which helps reduce background noise and enhances the specificity of the assay results.
Blocking Agent: While TBS-T primarily functions as a wash buffer, it can also be used in conjunction with blocking agents like BSA to further minimize non-specific binding during the assay.
Washing Solution: In immunohistochemistry and immunofluorescence, TBS-T serves as an effective washing solution that helps eliminate excess unbound antibodies from tissue sections or cell preparations, thereby improving the clarity of specific staining.
Dilution Buffer for Antibodies: TBS-T is also used as a dilution buffer for antibodies, providing an optimal environment for maintaining pH and ionic strength while preventing non-specific interactions.
Washing Buffer: Similar to its role in ELISA, TBS-T is utilized as a washing buffer in Western blotting to remove unbound antibodies from membranes, enhancing the specificity of the detected signals.
Composition:
Ingredients
Concentration
Tris-base
20mM
NaCl
150 mM
Tween 20
0.05%
Quality Control:
Appearance: Clear, Colorless Liquid.
pH: 7.4
Ultrapure: Filtered 0.1 micron Twice and 0.04 micron once in a sterile environment.
DNase activity: None was detected after incubating plasmid DNA and this product for 18 hr at room temperature.
RNase activity: None RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
Storage: Room temperature
Shelf life: 2 years
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA
