High Activity Brain Tumor Enzymatic Digestion Kit, 25T

Cat No.: RWD-DHBTE-2508
Number of reactions: 25T
   
   

Description

The High Activity Brain Tumor Enzymatic Digestion Kit enables gentle, rapid, and efficient preparation of high-viability single-cell suspensions from human brain tumors (clinical human brain tumor samples and in vitro transplanted brain tumor samples) and mouse brain tumors (including spontaneous and in vitro transplanted brain tumor samples). The protocol maximizes yield and cell viability while preserving critical cell surface antigen epitopes. The resulting single-cell suspensions are ideal for downstream applications such as primary culture or cell sorting.
 

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Mouse glioma

Using the RWD brain tumor gentle enzymatic digestion kit, single-cell suspension preparation instrument, and tissue processing tubes with standardized procedures, the quality of single-cell suspensions is measured using the RWD automatic cell counter.

To improve data reliability, AOPI staining was used to eliminate interference from impurities and debris when detecting target cells. Multiple parameters evaluate suspension quality from mouse glioma tissue. Results show cell viability up to 97%, total cell yield up to 8.53 × 10^6 per 100 mg; average diameter of all cells is 10.78 μm, live cells 10.85 μm, dead cells 8.61 μm.

Human glioma


 

Using the RWD brain tumor gentle enzymatic digestion kit, single-cell suspension preparation instrument, and tissue processing tubes with standardized procedures, the quality of harvested single-cell suspensions is measured with the RWD automated cell counter.

To improve data reliability, AOPI staining was used to eliminate interference from impurities and debris when detecting target cells, and multiple parameters monitor the quality of single-cell suspensions from mouse and human brain tumors. The results show cell viability up to 99%, total cell yield up to 2.16 × 10^6 cells per 100 mg; mean diameter of all cells is 12.50 μm, live cells 12.53 μm, and dead cells 9.93 μm.

Flow cytometry analysis


Results indicate that the kit enables detection of tumor cells and relevant immune subpopulations via surface markers CD44 and CD11b. These results demonstrate excellent tissue dissociation and antigen preservation, facilitating direct downstream cell sorting workflows.

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FAQs

Q: What are the storage requirements for the kit?
A: Storage temperature depends on enzyme type. Refer to the manual: store at either 4°C or –20°C as specified.

Q: What precautions are needed for tissue specimen preparation?
A: During tissue collection, carefully remove all blood vessels and residual clots. Rinse tissue thoroughly to ensure full cleanliness.

Q: What species/tissue types is this kit suitable for?
A: The kit is suitable for dissociating human and mouse brain tumor tissues.

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