The High Efficiency Debris Removal Kit is designed for the removal of debris from single-cell suspensions following enzymatic digestion. Application of this kit yields a cell suspension with a clean background and less debris. The kit is especially suited for efficient removal of debris from single-cell suspensions derived from adult mouse and rat brain, adult mouse heart, adult mouse liver, and various other tissues. Single cells prepared using this kit are recommended for downstream applications such as primary cell culture, flow analysis, and single-cell sequencing.
The background of the cell suspension is clean with few debris.
FAQs
Q: What are the storage conditions for tissue enzymatic dissociation kits?
A: Storage conditions depend on the specific enzymes included. Store kit components at 4°C or -20°C as directed in the kit instructions.
Q: How does the Defragmentation Kit work?
A: The kit separates cells from debris based on their different sedimentation rates. During centrifugation, cells and debris are partitioned, enabling efficient removal of debris.
Q: What is the correct order for liquid removal after centrifugation?
A: Following centrifugation, three layers are visible. Remove the middle layer first (the white, flocculent debris), then the upper layer.
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