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Human Nerve growth factor,NGF ELISA kit
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Human Nerve growth factor,NGF ELISA kit
Sandwich quantitative immunoassay for Human Nerve growth factor,NGF ELISA kit in human serum, plasma, cell culture supernates, tissue homogenates, cell lysates. available in multiple catalog sizes:
Note: Please send inquiries regarding Trial 24T orders to support@diagnocine.com.
| Uniprot No. | P01138 |
| Species | Homo sapiens (Human) |
| Sample types | serum, plasma, cell culture supernates, tissue homogenates, cell lysates. |
| Detection range | 6.86 pg/ml-5000 pg/ml. |
| Sensitivity | 6.86 pg/ml. |
| Assay time | 1-5h |
| Sample loading volume | 50-100μL |
| Detection wavelength | 450 nm |
| Assay principle | Sandwich (Quantitative) |
| Data analysis | Standard curve + Curve Expert software |
| Research area | Neuroscience |
| Storage condition | 2-8°C (see protocol for full details) |
| Shipping condition | 4 °C |
| Shelf life | 6 months |
10 business days
Processing + 3-5 days shipping
In Stock : USA
Worldwide shipping available
Antibody capture
450 nm detection
Standard curve
Quantification
In this sandwich ELISA, Human Nerve growth factor,NGF ELISA kit in the sample is captured between a pre-coated capture antibody and a detection antibody. Signal intensity is proportional to analyte concentration. Quantification uses a standard curve fitted with Curve Expert software, covering 6.86 pg/ml-5000 pg/ml. with a minimum detectable dose of 6.86 pg/ml..
The HumanNGF ELISA kit is designed for the detection and quantification of human NGF in serum, plasma, cell culture supernates, tissue homogenates, and cell lysates. Many performances including specificity, sensitivity, precision, linearity, and recovery of this kit, have been rigorously tested. More validation information is elucidated in the product instructions. The NGF in the sample or standard is immobilized on the pre-coated NGF antibody and then sandwiched by Biotin-conjugated NGF antibody. The forming Ag-Ab-Ag immune complex is labeled by HRP-avidin and develops a color reaction upon adding TMB substrate solution. The color intensity is positively correlated to the amount of NGF bound in the initial step and can be measured at 450 nm via a microplate reader. NGF is a neurotrophic factor essential for the growth, differentiation, and survival of sympathetic and sensory afferent neurons during development. It contributes to neuronal phenotype by regulating axonal guidance, gene transcription, neurotransmitter release, and synaptic plasticity. Numerous studies have shown that NGF plays a pivotal role in the modulation of nociception in adulthood. There are two receptors for NGF: p75 neurotrophin receptor (p75NTR) and tropomyosin receptor kinase A (TrkA). A study demonstrated that NGF-TrkA signaling-mediated communication between osteoblasts and sensory nerves is essential for load-induced bone formation in mice.
- Neuroimmune-endocrine events may lead to overactivity of sympathetic nervous system that triggers cascade of pathologic conditions in ovary in polycystic ovary syndrome (PCOS). Data suggest women with PCOS exhibit reduction of CRH and NGF; reduction of CRH/NGF may be under influence of sympathetic nervous system and may reflect deficit of neuronal stress-adaptation in PCOS patients. (CRH = corticotropin releasing hormone) PMID: 27908212
- Data show that the standardized nerve growth factor (NGF) concentration was negatively correlated with continuous pain, neuropathic pain and total score, and the standardized S100 proteins S100A8/A9 concentration was positively correlated with present pain intensity and continuous pain. PMID: 27936243
- NGF attenuates these responses-both in vivo and in vitro. Therefore, NGF therapy may represent a novel approach for the management of diabetic keratopathy. PMID: 27978558
- BDNF and NGF serum levels are reduced in the early and moderate glaucoma stages, suggesting the possibility that both factors could be further investigated as potential circulating biomarkers for the early detection of glaucoma. PMID: 28068360
- The results suggest an interaction between NGF, GDNF and MMP-9 during the transition to malignancy in prostate cancer (PC). Also this interaction may involve in regulating PC cell differentiation, tumor invasion, progression, and the agressiveness of PC. PMID: 28237042
- the effect and underlying mechanisms of NGF/BDNF on the production of NPW in PC12 cells and hypothalamus, is reported. PMID: 28249734
- a stage-related modulation of beta-NGF and its receptors in the inflammatory process of OA PMID: 28253191
- Results indicate that NGF inhibited CRT translocation induced by mitoxantrone. NGF effect on CRT translocation could have consequences in immunotherapy, potentially lessening the effectiveness of this type of treatment. PMID: 28260038
- NGF stimulates generation of neurons, but not neuronal progenitors from embryonic stem cells and affects the proportion of specific types of neurons in cultures of differentiating embryonic stem cells. PMID: 28364186
- Results show that NGF signalling is strongly linked to pathological and regenerative processes in human teeth. PMID: 28465581
- This review will briefly address the peripheral and central sensitization mechanisms of airway neurons and will then focus on NGF signaling and its role in cough hypersensitivity.[review] PMID: 28494216
- Findings suggest that IL-1beta and TNF-alpha regulate Nerve Growth Factor expression and production in synovial macrophages and fibroblasts in osteoarthritic joints. PMID: 28677145
- co-expression patterns of NGF and heme oxygenase-1 might be used as prognostic indicators for gastric carcinoma patients PMID: 28679437
- These results advance our knowledge of the conformational properties of proNGF and NGF and help provide a rationale for the diverse biological effects of NGF and proNGF at the molecular level. PMID: 28798232
- the anti-tumor activity of oleuropein against hepatocellular carcinoma could be attributed to influencing the pro-NGF/NGF balance via affecting MMP-7 activity without affecting the gene expression of NGF PMID: 29476769
- Single nucleotide polymorphisms in NGF gene (rs6330) and NGFR gene (rs2072446 and rs734194) are associated with ischemic stroke. The NGF rs6330*T and NGFR rs2072446*T minor alleles might be nominated as a risk factor for developing ischemic stroke and NGFR rs734194*G minor allele as a protective against this disease at least in Armenian population PMID: 29499660
- the effects of secretome-enriched conditioned medium (CM) of neuronally differentiated PC12 cells on the neuronal differentiation of human mesenchymal stem cells via NGF like mechanism, is reported. PMID: 29527653
- Neurotrophic factors and hippocampal activity in PTSD PMID: 29799860
- SNRPA may contribute to GC progression via NGF and could be a prognostic biomarker for GC. PMID: 30039889
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| |||||||
pg/ml | OD1 | OD2 | Average | Corrected | |||
5000 | 2.813 | 2.864 | 2.839 | 2.687 | |||
1666.7 | 2.346 | 2.351 | 2.349 | 2.197 | |||
555.6 | 1.722 | 1.752 | 1.737 | 1.585 | |||
185.2 | 1.133 | 1.148 | 1.141 | 0.989 | |||
61.7 | 0.670 | 0.683 | 0.677 | 0.525 | |||
20.6 | 0.483 | 0.491 | 0.487 | 0.335 | |||
6.86 | 0.274 | 0.283 | 0.279 | 0.127 | |||
0 | 0.150 | 0.153 | 0.152 |
| |||
To assess the linearity of the assay, samples were spiked with high concentrations of human NGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | ||||
1:1 | Average % | 95 | ||||
Range % | 90-100 | |||||
1:2 | Average % | 94 | ||||
Range % | 90-98 | |||||
1:4 | Average % | 92 | ||||
Range % | 87-100 | |||||
1:8 | Average % | 89 | ||||
Range % | 84-95 | |||||
The recovery of human NGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 89 | 85-97 | ||||
EDTA plasma (n=4) | 92 | 88-99 | ||||
