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Pyruvate Kinase KS1, Thermostable

Product#: FNK-PYK-88-01

$241.17

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Pyruvate Kinase KS1, Thermostable

Cat.No. FNK-PYK-88-01
Lot No. 0-H302
Protein conc. 4.9 mg/ml
Size 100 Units
Volume 1 ml
Form 50 mM Tris-HCl (pH 7.5), 50 mM NaCl
Storage -20 oC
Activity 145 U/ml
Notes For research use only

EC No. 2.7.1.40
Substrate phosphoenolpyruvic acid
Reaction product pyruvic acid
Reaction temperature ~85℃
Reaction pH 5.0~8.0

Description

Enzymes work at high temperature with high reactivity The "Thermostable Enzyme".

Generally, enzymes in the living body are denatured and lose activities at the temperature over 50 - 60°C.
However, the thermostable enzymes obtained from special microorganisms (hyper-thermophilic microorganisms) alive at high temperature (over 70°C) have unique characteristics.

Using regular enzymes as a catalyst in chemical reactions are not cost effective due to its low stability and needs of additional enzyme.
By using these thermostable enzymes, we can expect not only cost saving but also other advantages - especially in the field of fine chemicals.

Features of Thermostable Enzyme

Highly stable and durable for long term use
High reactivity at high temperature (70 - 100°C)
Resistance to organic solvent is expected
Special instruments to inhibit bacterial growth are not required.
Reaction speficity is much higher compared with chemical reaction
Speed of reaction can be accelerated

Activity measurement

Reaction mix (20 mM Potassium phosphate buffer (pH 7.0), 5 mM Phosphoenolpyruvate, 0.3 mM NADH, 5 mM MgCl2, 1 mM ADP, 0.1 mM TPP, 4 U/ml Pyruvate decarboxylase、46 U/ml Alcohol dehydrogenase and appropriate amount of the enzyme) was incubated at 50 oC and A340 was monitored. One unit is defined as the amount of the enzyme oxidizing 1 µmol of NADH (e340=6.22 mM^-1 cm^-1 ) per 1 minute using Phosphoenolpyruvate as a substrate.