This Human ADP ELISA Kit was designed for the quantitative measurement of Human ADP protein in serum, plasma, cell culture supernates, tissue homogenates, urine. It is a Sandwich ELISA kit, its detection range is 1.562 ng/mL-100 ng/mL and the sensitivity is 1.102 ng/mL.

30 kDa adipocyte complement related protein ELISA Kit; 30 kDa adipocyte complement-related protein ELISA Kit; ACDC ELISA Kit; Acrp 30 ELISA Kit; ACRP30 ELISA Kit; ADIPO_HUMAN ELISA Kit; Adipocyte ELISA Kit; Adipocyte C1q and collagen domain containing protein ELISA Kit; Adipocyte complement related 30 kDa protein ELISA Kit; Adipocyte complement related protein of 30 kDa ELISA Kit; Adipocyte complement-related 30 kDa protein ELISA Kit; adipocyte-specific secretory protein ELISA Kit; Adiponectin ELISA Kit; Adiponectin precursor ELISA Kit; adiponectin; C1Q and collagen domain containing ELISA Kit; Adipoq ELISA Kit; Adipose most abundant gene transcript 1 ELISA Kit; Adipose most abundant gene transcript 1 protein ELISA Kit; Adipose specific collagen like factor ELISA Kit; ADIPQTL1 ELISA Kit; ADPN ELISA Kit; APM 1 ELISA Kit; apM-1 ELISA Kit; APM1 ELISA Kit; C1q and collagen domain-containing protein ELISA Kit; GBP 28 ELISA Kit; GBP28 ELISA Kit; Gelatin binding protein ELISA Kit; Gelatin binding protein 28 ELISA Kit; gelatin-binding protein 28 ELISA Kit; Gelatin-binding protein ELISA Kit; OTTHUMP00000210047 ELISA Kit

Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.

• A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human ADP antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
• Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
• One vial Biotin-labeled ADP antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
• One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
• One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the high concentration Biotin-antibody to an appropriate working solution.
• One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the high concentration HRP-avidin solution to an appropriate solution.
• One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
• One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
• One vial TMB Substrate (10 ml/bottle) --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
• One vial Stop Solution (10 ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
• Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
• An instruction manual

• A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
• An incubator can provide stable incubation conditions up to 37°C±5°C.
• Centrifuge
• Vortex
• Squirt bottle, manifold dispenser, or automated microplate washer
• Absorbent paper for blotting the microtiter plate
• 50-300ul multi-channel micropipette
• Pipette tips
• Single-channel micropipette with different ranges
• 100ml and 500ml graduated cylinders
• Deionized or distilled water
• Timer
• Test tubes for dilution