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Human Interferon γ (IFN-γ) ELISA Kit
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Human Interferon γ (IFN-γ) ELISA Kit
sandwich quantitative immunoassay for Human Interferon γ (IFN-γ) in human serum, plasma, tissue homogenates, cell culture supernates available in multiple catalog sizes:
Note: Please send inquiries regarding Trial 24T orders to support@diagnocine.com.
| Uniprot No. | P01579 |
| Species | Homo sapiens (Human) |
| Sample types | serum, plasma, tissue homogenates, cell culture supernates |
| Detection range | 7.8 pg/ml-500 pg/ml |
| Sensitivity | 1.95 pg/ml |
| Assay time | 1-5h |
| Sample loading volume | 50-100μL |
| Detection wavelength | 450 nm |
| Assay principle | sandwich (Quantitative) |
| Data analysis | Standard curve + Curve Expert software |
| Research area | Immunology |
| Storage condition | 2-8°C (see protocol for full details) |
| Shipping condition | 4 °C |
| Shelf life | 6 months |
10 business days
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In Stock : USA
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Antibody capture
450 nm detection
Standard curve
Quantification
In this sandwich ELISA, Human Interferon γ (IFN-γ) in the sample is captured between a pre-coated capture antibody and a detection antibody. Signal intensity is proportional to analyte concentration. Quantification uses a standard curve fitted with Curve Expert software, covering 7.8 pg/ml-500 pg/ml with a minimum detectable dose of 1.95 pg/ml.
This ELISA kit provides quantitative measurement of human interferon-gamma (IFN-γ), a pivotal type II interferon produced primarily by T cells and NK cells that orchestrates cell-mediated immune responses. Because IFN-γ levels can span from near-undetectable baseline concentrations in healthy plasma to sharply elevated values during active immune activation, the kit is engineered to capture this dynamic range across diverse experimental contexts.
Detection Range: The 7.8–500 pg/ml range aligns with the physiological and pathological concentrations of circulating IFN-γ, from low resting levels in healthy serum to elevated concentrations seen during infection, graft rejection, or Th1-driven inflammation. Mitogen-stimulated PBMC supernatants may require dilution to fall within range, while unstimulated samples can typically be assayed neat.
- this study demonstrates reduced IFN-gamma production in chronic brucellosis patients PMID: 28919584
- STAT1b plays a key role in enhancing the tumor suppressor function of STAT1a, in ESCC, in a manner that can be amplified by IFN-gamma PMID: 28981100
- Phosphorylation of T-bet by RSK2 is required for IFNgamma expression for attenuation of colon cancer metastasis and growth. PMID: 29133416
- an electrophoretic mobility shift assay showed that signal transducers and activators of transcription 1 (STAT1) attach to the GAS motif on the human STING promoter region. This indicates that IFN-gamma/Janus kinases/STAT1 signaling is essential for the STING upregulation in human keratinocytes. PMID: 29143896
- SNX8 mediates IFNG-triggered non-canonical signaling pathway and host defense against Listeria monocytogenes. PMID: 29180417
- Impaired IFNgamma-Signaling and Mycobacterial Clearance in IFNgammaR1-Deficient Human iPSC-Derived Macrophages. PMID: 29249666
- H pylori expression of cgt reduces cholesterol levels in infected gastric epithelial cells and thereby blocks IFNG signaling, allowing the bacteria to escape the host inflammatory response. PMID: 29273450
- IFN-gamma can promote cancer immunoevasion. (Review) PMID: 29283429
- Association Between the Interferon Gamma 874 T/A Polymorphism and the Severity of Valvular Damage in Patients with Rheumatic Heart Disease. PMID: 29332266
- this study shows that elevated levels of interferon-gamma are associated with high levels of Epstein-Barr virus reactivation in patients with the intestinal type of gastric cancer PMID: 29349089
- No correlation was observed between interferon gamma mRNA/protein levels and recurrent depressive disorders. PMID: 29367100
- epigenetic silencing by single CpG methylation determines differential IL18BP inducibility in monocytic versus epithelial cells. T PMID: 29409936
- IFNgamma induces a rapid activation of aerobic glycolysis followed by a reduction in oxidative phosphorylation in M1 macrophages. PMID: 29463472
- Dysregulation of the IFN-gamma-STAT1 signaling pathway in a cell line model of large granular lymphocyte leukemia PMID: 29474442
- Systemic IFN activation is associated with higher activity only in the ESSDAI biological domain but not in other domains or the total score. Our data raise the possibility that the ESSDAI biological domain score may be a more sensitive endpoint for trials targeting either IFN pathway. PMID: 29474655
- This study contributes to clarification of the previously inconsistent prognostic performance of IFNgamma by providing the first prognostic evaluation with long follow-up, time-dependence assessment and absence of any chemotherapy influence. PMID: 29478965
- IFN gamma induced upregulation of BCL6 was dependent on the classical STAT1 signaling pathway, and affected both major BCL6 variants. Interestingly, although IFN alpha induced stronger STAT1 phosphorylation than IFN gamma, it only slightly upregulated BCL6 in multiple myeloma lines. PMID: 29510136
- results suggested that IFN-gamma induces autophagy-associated apoptosis in CRC cells via inducing cPLA2-dependent mitochondrial ROS production. PMID: 29551681
- IFN-gamma increases free ISG15 levels in the cytoplasm and ISGylation in the nucleus and cytoplasm, but in a manner distinct between MCF-7 and MDA-MB-231cells. PMID: 29626479
- hypomethylation of the IFNG promoter is significantly related to the risk of essential hypertension PMID: 29643275
- These results demonstrated that the IFNGinduced immunosuppressive properties of B7H1 in human BM and WJMSCs were mediated by STAT1 signaling, and not by PI3K/RACalpha serine/threonineprotein kinase signaling PMID: 29901104
- results suggest, the interplay of pro-inflammatory cytokines IFN-gamma derived from CD4+T lymphocytes and TNF-alpha from CD14+ cells has no direct additive impact on parasite replication but induces IL-4 production. PMID: 29953494
- Results provide evidence that rs2069707 locus SNPs of IFN-gamma is a risk factor for contracting tuberculous pericarditis. PMID: 30017738
- High IFNG expression is associated with Chronic Periodontitis. PMID: 30051674
- High expression of IFNG is associated with breast cancer. PMID: 30336781
Intra-assay Precision (Precision within an assay): CV%<8% | |||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||
Three samples of known concentration were tested in twenty assays to assess. | |||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ||||||
pg/ml | OD1 | OD2 | Average | Corrected | ||
500 | 2.046 | 2.058 | 2.052 | 1.902 | ||
250 | 1.537 | 1.444 | 1.491 | 1.341 | ||
125 | 1.027 | 0.987 | 1.007 | 0.857 | ||
62.5 | 0.648 | 0.635 | 0.642 | 0.492 | ||
31.2 | 0.456 | 0.432 | 0.444 | 0.294 | ||
15.6 | 0.342 | 0.345 | 0.344 | 0.194 | ||
7.8 | 0.241 | 0.254 | 0.248 | 0.098 | ||
0 | 0.151 | 0.149 | 0.150 |
| ||
To assess the linearity of the assay, samples were spiked with high concentrations of human IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||
| Sample | Serum(n=4) | |||
1:1 | Average % | 92 | |||
Range % | 87-96 | ||||
1:2 | Average % | 95 | |||
Range % | 92-99 | ||||
1:4 | Average % | 91 | |||
Range % | 87-94 | ||||
1:8 | Average % | 89 | |||
Range % | 86-93 | ||||
The recovery of human IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||
Sample Type | Average % Recovery | Range | |||
Serum (n=5) | 91 | 88-96 | |||
EDTA plasma (n=4) | 95 | 89-99 | |||
