Flamma® 496 Thiol
Size of product (mg)
-
1 mg
-
5 mg
-
25 mg
Flamma® 496 Thiol
Cat. No. List below
Description
Key features:
1. Excitation/Emission maxima: 494/520 nm
2. High extinction coefficient: ≥ 70,000 cm?¹M?¹
3. Thiol functional group attached through a spacer
4. Yellow solid appearance
5. Molecular weight: 473.35 g/mol
6. Soluble in DMF and DMSO
Applications:
1. Bioimaging and fluorescence microscopy
2. Labeling of biomolecules through disulfide bond formation
3. Reference standard for dye-conjugates
4. Potential use in flow cytometry and fluorescence-based assays
5. Cellular labeling and detection
Advantages:
1. Spectral similarity to popular dyes (Alexa488, FITC, Cy2)
2. Excellent optical properties when excited with a 488 nm laser line
3. Versatile labeling options through disulfide bond formation
4. Stable fluorescence signal for extended imaging sessions
5. High sensitivity for detecting low-abundance biological structures
Flamma® Fluors 496 Thiol features a unique structure where the thiol group is attached to the fluorophore through a spacer, enhancing its reactivity and flexibility in various experimental conditions. The dye's spectral properties position it in the bright green region of the visible spectrum, making it an excellent choice for multicolor imaging experiments.
- Fluorophore: Flamma® Fluors 496
- Functional group: Thiol
- Excitation/Emission Max.(nm): 494/520
- Spectrally similar dyes: Alexa488, FITC, Cy2
- Extinction coefficient: ≥ 70,000 cm-1M-1
- Appearance: Yellow Solid
- Molecular Weight: 473.35 g/mol
- Solubility: DMF, DMSO
- Storage conditions: -20 ℃, protect from light
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| CWT1001 | Flamma® 496 Thiol | 494 | 520 | Alexa488, FITC, Cy2 |
| CWT1058 | Flamma® 552 Thiol | 550 | 564 | Alexa555, DyLight549, Cy3, ATTO550 |
| KWT1415 | Flamma® 581 Thiol | 578 | 593 | Alexa594, DyLight594 |
| KWT1042 | Flamma® 648 Thiol | 648 | 663 | Alexa647, DyLight650, Cy5 |
| PWT1415 | Flamma® 675 Thiol | 674 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT, CF680 |
| PWT1215 | Flamma® 749 Thiol | 749 | 774 | Alexa750, DyLight755, Cy7.5, IRDye750 |
| PWT1515 | Flamma® 774 Thiol | 774 | 800 | Cy7.5, DyLight800, IRDye800 |
Background
Flamma® Fluors
BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.
- Covering the full spectral range from UV to NIR
- Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
- High quantum yields and photostability
- High purity and compatible with most of biomolecules
Flamma® Fluors Thiol
Flamma® Fluors thiol dyes have an attached primary thiol, which connected through a spacer. These thiol dyes can be labeled to biomolecules through disulfide bond formation with thiol of cysteine residue.
Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma® Fluors
Figure 2. Immunofluorescence imaging and in situ hybridization imaging
Figure 3. Fluorescence images of Flamma® 749 (upper) and Flamma® 774 (bottom) carboxylic acid injected mouse model
Citation & Reference
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