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Phosphate Based Lysis/Extraction Buffers

Product#: PhosphateBasedLysis/ExtractionBuffers
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science Phosphate-Stabilized · pH 7.4 · Quadruple-Stage Filtered

FluxMPS™ Phosphate Based Lysis/Extraction Buffers

This is a family overview, not an orderable page. Pick the buffer — or the pre-blended protease-inhibitor version — and click View to reach its own product page.

Phosphate Based Lysis/Extraction Buffers — Catalog · 9 products · 12 catalog entries
Grouped into base buffers and ready-to-use protease-inhibitor pre-blends. Every size is listed on its own row — click View for the product page.
Name Cat. No. Size Product Page
water_dropPhosphate Based Lysis/Extraction Buffers · 3 products · 6 sizes
Base detergent buffers. pH 7.4 · clear, colorless liquid · filtered 0.1 µm twice, then 0.04 µm twice, in a sterile environment. See the filtration architecture.
Dulbecco’s PBS NP-40 Buffer DCP-DPBSNP401X_500 ml 500 ml Viewarrow_forward
Dulbecco’s PBS NP-40 Buffer DCP-DPBSNP401X_1000 ml 1000 ml Viewarrow_forward
PBS Triton X-100 Buffer DCP-PBSTX1X_500 ml 500 ml Viewarrow_forward
PBS Triton X-100 Buffer DCP-PBSTX1X_1000 ml 1000 ml Viewarrow_forward
PBS NP-40 Buffer DCP-PBSNP1X_500 ml 500 ml Viewarrow_forward
PBS NP-40 Buffer DCP-PBSNP1X_1000 ml 1000 ml Viewarrow_forward
inventory_2Protease Inhibitor Cocktail Pre-Blends · 6 products
Ready-to-use combinations of the seven-component protease inhibitor cocktail with a phosphate-based buffer. See the cocktail components.
Protease Inhibitor Cocktail with PBS Triton-X Buffer DCP-PICPTX1X 10 x 10 ml Viewarrow_forward
Protease Inhibitor Cocktail with PBS NP-40 Buffer DCP-PICPNP1X 10 x 10 ml Viewarrow_forward
Protease Inhibitor Cocktail with PBS Tween 20 Buffer DCP-PICPT201X 10 x 10 ml Viewarrow_forward
Protease Inhibitor Cocktail with DPBS Triton-X Buffer DCP-PICDPTX1X 10 x 10 ml Viewarrow_forward
Protease Inhibitor Cocktail with DPBS NP-40 Buffer DCP-PICDPNP1X 10 x 10 ml Viewarrow_forward
Protease Inhibitor Cocktail with DPBS Tween 20 Buffer DCP-PICDPT201X 10 x 10 ml Viewarrow_forward

Not sure which one? Compare by detergent · See the cocktail components · Read the shared specifications · Read the FAQ

Family Snapshot

What the phosphate base buys you

Phosphate-based lysis/extraction buffers provide an effective option for protein isolation, particularly when physiological conditions need to be maintained or when working with phosphate-binding proteins. Their ability to maintain stable pH and compatibility with various additives make them a valuable tool in biochemical research.

  • Nine products, twelve catalog entries. Three base lysis/extraction buffers in two sizes each, plus six ready-to-use protease inhibitor cocktail pre-blends.
  • Three detergents across the family: NP-40, Triton X-100, and Tween 20 — on a PBS or Dulbecco’s PBS (DPBS) phosphate base.
  • Specified at pH 7.4 and supplied as a clear, colorless liquid.
  • Quadruple-stage filtration: filtered 0.1 micron twice and 0.04 micron twice, in a sterile environment.
  • Stored at 4 °C.
  • The cocktail does the protecting. Detergents disrupt cell membranes and solubilize proteins, while the carefully formulated protease inhibitor cocktail prevents protein degradation during the lysis process.
  • Seven inhibitor components covering serine, cysteine, aspartic, amino- and metalloprotease classes: AEBSF·HCl, Aprotinin, Bestatin, E-64, EDTA, Leupeptin, and Pepstatin A.
0.04 µm Filtered Sterile Environment Fill RUO
FAMILY REFERENCE · PHOSPHATE BASED LYSIS/EXTRACTION BUFFERS
Every published specification for this family, in one place
  • Products on this page9
  • Base lysis/extraction buffers3
  • Protease inhibitor pre-blends6
  • pH7.4
  • AppearanceClear, Colorless Liquid
  • Filtration0.1 µm ×2 + 0.04 µm ×2
  • Fill environmentsterile environment
  • Storage4 °C
  • Base buffer sizes500 ml · 1000 ml
  • Pre-blend size10 x 10 ml
Why a Phosphate Base

Six reasons this buffer family exists

Each card below is one property the source attributes to this family — the reasons a phosphate base gets chosen over another buffering system.

balance

Physiological conditions maintained

Phosphate-based buffers are an effective option for protein isolation particularly when physiological conditions need to be maintained — the reason the family is specified at pH 7.4 on a PBS or DPBS base.

link

Phosphate-binding proteins

The other case the source calls out explicitly: work with phosphate-binding proteins, where the buffering ion is part of the experimental question rather than incidental to it.

show_chart

Stable pH

The ability to maintain stable pH is one of the two properties the source names as making these buffers valuable in biochemical research. The published figure for this family is pH 7.4.

tune

Compatibility with additives

The second named property: compatibility with various additives. That is what makes the pre-blended protease inhibitor versions in the second catalog band possible in the first place.

blur_on

Detergents open the cell

The method utilizes detergents to disrupt cell membranes and solubilize proteins. Across this family that detergent is NP-40, Triton X-100, or Tween 20, depending on the product you pick.

shield

The cocktail prevents degradation

The carefully formulated protease inhibitor cocktail prevents protein degradation during the lysis process — a powerful tool for efficient protein extraction while preserving protein integrity.

Ready-to-use removes a pipetting step from the critical moment

Lysis is the point where proteases meet your target. The pre-blended products in the second catalog band ship the seven-component cocktail already combined with the phosphate-based buffer, so the inhibitor is present the instant the detergent starts working rather than being added in a separate step.

7
inhibitor components in the cocktail, spanning serine, cysteine, aspartic, amino- and metalloprotease classes
6
ready-to-use cocktail-plus-buffer combinations, at 10 x 10 ml each
Filtration Architecture

Quadruple-stage filtration, in a sterile environment

The published sterility statement for this family is a four-pass membrane process: 0.1 micron twice, then 0.04 micron twice. A lysis buffer is added directly to the sample and travels with it downstream, so whatever the buffer carries becomes part of the lysate.

  1. 1

    0.1 µm Pre-filtration I

    First pass through a 0.1-micron membrane.

  2. 2

    0.04 µm Pre-filtration II

    First pass through a 0.04-micron membrane.

  3. 3

    0.1 µm Sterile-filtration I

    Second pass through a 0.1-micron membrane, performed in a sterile environment.

  4. 4

    0.04 µm Sterile-filtration II — Final Polish

    Final 0.04-micron polishing pass in a sterile environment. The buffer is delivered as a clear, colorless liquid.

Why the polish matters in a lysate

An ultra-filtered, ultra-low-particulate buffer keeps background out of colorimetric assays, gel electrophoresis, and quantitative immunodetection, and keeps it out of microfluidic and organ-on-a-chip (OoC) sample handling — where a particulate that would be invisible in a cuvette can block a channel.

0.04
micron — the final polishing membrane rating
×4
membrane passes: 0.1 micron twice, then 0.04 micron twice
Filtration is stated per product. Filtration method and pass count can change per order — for the process applied to a specific lot, request the documentation at support@diagnocine.com.
Quadruple-stage filtration architecture for Diagnocine FluxMPS phosphate based lysis and extraction buffers: two 0.1 micron membrane passes followed by two 0.04 micron polishing passes in a sterile environment, producing ultra-low-particulate pH 7.4 PBS and DPBS detergent buffers for protein extraction, Western blot and immunoprecipitation workflows
Figure 1. Quadruple-stage filtration — 0.1 micron twice followed by 0.04 micron twice, in a sterile environment — as published for the Phosphate Based Lysis/Extraction Buffers family. © Diagnocine®
Protease Inhibitor Cocktail

The seven components and what each one stops

These ready-to-use combinations of a protease inhibitor cocktail with various lysis buffers provide a powerful tool for efficient protein extraction while preserving protein integrity. This method utilizes detergents to disrupt cell membranes and solubilize proteins, while the carefully formulated protease inhibitor cocktail prevents protein degradation during the lysis process.

Component What it inhibits
AEBSF·HCl A serine protease inhibitor
Aprotinin Targets serine proteases and has anti-inflammatory properties
Bestatin An aminopeptidase inhibitor[4]
E-64 A cysteine protease inhibitor[2]
EDTA A metalloproteinase inhibitor
Leupeptin Inhibits both serine and cysteine proteases[5]
Pepstatin A An aspartic protease inhibitor[3]
One consequence of the EDTA in the cocktail. The source states that EDTA is present as a metalloproteinase inhibitor, and that it works by binding metal ions. That same chelation is not selective for proteases: any downstream step that depends on a metal-dependent enzyme, or on free divalent cations, sees the EDTA too. Worth checking against your workflow before the lysate goes forward. For a formulation question, contact support@diagnocine.com.
Compare by Detergent

Which products carry which detergent

The same nine products, regrouped by the detergent in the formulation rather than by catalog band. Every entry is reproduced from the catalog above.

NP-40 · 4 products · PBS and DPBS bases
  • Dulbecco’s PBS NP-40 Buffer — DCP-DPBSNP401X · 500 ml, 1000 ml
  • PBS NP-40 Buffer — DCP-PBSNP1X · 500 ml, 1000 ml
  • Protease Inhibitor Cocktail with PBS NP-40 Buffer — DCP-PICPNP1X · 10 x 10 ml
  • Protease Inhibitor Cocktail with DPBS NP-40 Buffer — DCP-PICDPNP1X · 10 x 10 ml
Triton X-100 · 3 products · PBS and DPBS bases
  • PBS Triton X-100 Buffer — DCP-PBSTX1X · 500 ml, 1000 ml
  • Protease Inhibitor Cocktail with PBS Triton-X Buffer — DCP-PICPTX1X · 10 x 10 ml
  • Protease Inhibitor Cocktail with DPBS Triton-X Buffer — DCP-PICDPTX1X · 10 x 10 ml
Tween 20 · 2 products · PBS and DPBS bases
  • Protease Inhibitor Cocktail with PBS Tween 20 Buffer — DCP-PICPT201X · 10 x 10 ml
  • Protease Inhibitor Cocktail with DPBS Tween 20 Buffer — DCP-PICDPT201X · 10 x 10 ml
The source states no selection criterion between the three detergents, or between the PBS and DPBS bases. The grouping above reports which product carries which; it does not rank them. For help matching a detergent and base to a specific target, contact support@diagnocine.com.
Shared Specifications

What every product in this family has in common

The published specification block for the Phosphate Based Lysis/Extraction Buffers family, reproduced value for value.

Published specifications
Appearance Clear, Colorless Liquid
pH 7.4
Sterility Filtered 0.1 micron twice and 0.04 micron twice, in a sterile environment
Storage 4 °C
FAQ

Frequently asked questions

The questions that come up most often when choosing within this family.

The source names two cases specifically: when physiological conditions need to be maintained, and when working with phosphate-binding proteins. Its ability to maintain a stable pH and its compatibility with various additives are what make it a valuable tool in biochemical research.
Appearance: clear, colorless liquid. pH: 7.4. Sterility: filtered 0.1 micron twice and 0.04 micron twice, in a sterile environment. Storage: 4 °C. These apply across the family; individual product pages carry any product-specific detail.
Through a quadruple-stage membrane process: 0.1 micron twice, then 0.04 micron twice, carried out in a sterile environment. Because a lysis buffer is added straight to the sample and stays with it through every downstream step, the polishing pass keeps particulate background out of the lysate itself. Filtration method and pass count can change per order — request lot documentation at support@diagnocine.com.
The family carries all three. NP-40 appears in Dulbecco’s PBS NP-40 Buffer, PBS NP-40 Buffer, and two cocktail pre-blends. Triton X-100 appears in PBS Triton X-100 Buffer and two cocktail pre-blends. Tween 20 appears in two cocktail pre-blends. The source states no criterion for choosing between them, and none is invented here — for help matching a detergent to a specific target, contact support@diagnocine.com.
Seven components: AEBSF·HCl, a serine protease inhibitor; Aprotinin, which targets serine proteases and has anti-inflammatory properties; Bestatin, an aminopeptidase inhibitor; E-64, a cysteine protease inhibitor; EDTA, a metalloproteinase inhibitor; Leupeptin, which inhibits both serine and cysteine proteases; and Pepstatin A, an aspartic protease inhibitor.
The pre-blends are ready-to-use combinations of the cocktail with the buffer, described as a powerful tool for efficient protein extraction while preserving protein integrity. The detergent disrupts the cell membrane and solubilizes proteins while the cocktail prevents protein degradation during the lysis process — so the inhibitor is already present at the moment lysis releases the sample’s own proteases.
The three base lysis/extraction buffers — Dulbecco’s PBS NP-40 Buffer (DCP-DPBSNP401X), PBS Triton X-100 Buffer (DCP-PBSTX1X), and PBS NP-40 Buffer (DCP-PBSNP1X) — are each listed in 500 ml and 1000 ml. All six protease inhibitor cocktail pre-blends are listed at 10 x 10 ml. Every size appears on its own row in the catalog at the top of this page.
Supporting Literature

Primary sources behind the chemistry

The source description carries no reference list. The entries below are the primary reports for the detergent mechanism and for the cocktail components whose original literature could be verified.

  1. Helenius, A., & Simons, K. (1975). Solubilization of membranes by detergents. Biochimica et Biophysica Acta (BBA) – Reviews on Biomembranes, 415(1), 29–79. — How detergent class, concentration, and critical micelle concentration govern membrane solubilization; the basis for every detergent-based lysis buffer.doi:10.1016/0304-4157(75)90016-7
  2. Hanada, K., Tamai, M., Yamagishi, M., Ohmura, S., Sawada, J., & Tanaka, I. (1978). Isolation and characterization of E-64, a new thiol protease inhibitor. Agricultural and Biological Chemistry, 42(3), 523–528. — The original isolation of E-64 from Aspergillus japonicus, reported as a specific, irreversible inhibitor of thiol (cysteine) proteases.doi:10.1271/bbb1961.42.523
  3. Umezawa, H., Aoyagi, T., Morishima, H., Matsuzaki, M., & Hamada, M. (1970). Pepstatin, a new pepsin inhibitor produced by Actinomycetes. The Journal of Antibiotics, 23(5), 259–262. — The original report of pepstatin, the reference inhibitor of aspartic proteases.
  4. Umezawa, H., Aoyagi, T., Suda, H., Hamada, M., & Takeuchi, T. (1976). Bestatin, an inhibitor of aminopeptidase B, produced by actinomycetes. The Journal of Antibiotics, 29(1), 97–99. — The original report of bestatin as an aminopeptidase inhibitor.
  5. Aoyagi, T., Takeuchi, T., Matsuzaki, A., Kawamura, K., Kondo, S., Hamada, M., Maeda, K., & Umezawa, H. (1969). Leupeptins, new protease inhibitors from actinomycetes. The Journal of Antibiotics, 22(6), 283–286. — The original report of the leupeptins, which inhibit both serine and cysteine proteases.
Buffer selection support. For help matching a detergent, base, or inhibitor format to a specific target or protocol, for customization requests, or for lot documentation, contact support@diagnocine.com. Ready to order? Back to the Phosphate Based Lysis/Extraction Buffers catalog.

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