Protease Inhibitor Cocktail with PBS Tween 20 Buffer
Cat. No. DCP-PICPT201X
Size: 10x10 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
Description:
This Ready-to-use combination of a protease inhibitor cocktail with PBS Tween 20 Buffer is a comprehensive solution designed for efficient cell lysis and protein extraction while preserving protein integrity. The addition of PBS (Phosphate Buffered Saline) provides a physiological pH and ionic strength, while Tween 20 acts as a mild detergent to aid in cell lysis and protein solubilization. This formulation is particularly useful for maintaining protein integrity during various biochemical procedures, including Western blotting, immunoprecipitation, and enzymatic assays. The combination of protease inhibitors with PBS Tween 20 Buffer offers researchers a convenient and effective solution for preserving native cellular proteins and studying their functions without the interference of proteolytic degradation.
The cocktail contains AEBSF.HCl, a serine protease inhibitor; Aprotinin, which targets serine proteases and has anti-inflammatory properties; Bestatin, an aminopeptidase inhibitor; E-64, a cysteine protease inhibitor; EDTA, a metalloproteinase inhibitor; Leupeptin, which inhibits both serine and cysteine proteases; and Pepstatin A, an aspartic protease inhibitor. This cocktail effectively minimizes protein degradation in a wide range of experimental procedures, including cell lysis, protein purification, and enzymatic assays. The carefully balanced concentrations of each component ensure optimal inhibition of proteolytic activity while maintaining compatibility with most downstream applications, making it an invaluable tool for researchers working with sensitive protein samples.
Advantages:
- Protease inhibitor cocktails contain multiple inhibitors targeting different classes of proteases, providing broad-spectrum protection against protein degradation.
- The PBS Tween 20 buffer effectively lyses cells and solubilizes proteins, while the protease inhibitors prevent degradation.
- Improves the yield of intact proteins for downstream applications.
- By preventing proteolysis, this combination helps maintain the native structure and function of proteins, which is crucial for accurate analysis in various biochemical and molecular biology experiments.
Application:
- Western Blotting: Protease inhibitors are crucial for preserving protein samples for accurate analysis.
- Protein Extraction and Preservation: Protease inhibitor cocktails are added to lysis buffers (such as PBS with Tween 20) to protect proteins during cell lysis and extraction. They help maintain the integrity of proteins by preventing degradation by endogenous proteases released during cell disruption.
- Drug Development: Protease inhibitors play a role in studying protein targets and developing new therapeutics.
- Cell and Tissue Culture: This mixture is valuable in cell and tissue culture applications, helping to maintain protein integrity during experiments involving cell funtion studies, natural cell regulation research and microbial defense investigations.
Composition:
| Ingredients |
Concentration |
| AEBSF.HCl |
100 uM |
| Aprotinin |
0.8 uM |
| Bestatin |
0.05 mM |
| E-64 |
1 uM |
| EDTA |
5 mM |
| Leupeptin |
0.01 mM |
| Pepstatin A |
1 uM |
| Tween 20 |
0.1% |
| Potassium Chloride |
2.7 mM |
| Sodium Chloride |
137 mM |
| Sodium Phosphate dibasic |
10 mM |
| Potassium Phosphate monobasic |
1.8 mM |
Quality Control:
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Appearance: Clear solution.
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Sterility: Filtered 0.1 micron Twice and 0.04 micron once in a sterile environment.
-
DNase activity: None was detected after incubation of plasmid DNA, and this product was used for 18 hours at room temperature.
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RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
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Storage: -80 ℃
-
Shelf life: 3 months
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of other tracking dyes, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
