Dulbecco’s PBS NP-40 Buffer [1X]
Cat No.: DCP-DPBSNP401X
Size: 500 mL & 1 L
Non-Sterile; Ultrapure, Filtered 0.1-micron membrane Once and 0.04-micron membrane Once.
Description
The addition of NP-40 to Dulbecco's PBS creates a versatile buffer that combines the osmotic balance and pH stability of DPBS with the protein extraction and membrane permeabilization properties of NP-40. This makes it a valuable tool for a wide range of biological and biochemical applications, particularly those involving protein extraction and analysis. NP-40 is a relatively mild detergent, which can help preserve the activity and structure of many proteins during extraction and analysis. NP-40 is especially effective at solubilizing membrane proteins, which are often difficult to extract due to their hydrophobic nature. This property makes the buffer valuable for studies involving membrane-associated proteins.
Applications
-
Cell Lysis and Protein Extraction: NP-40 is effective in lysing cells and solubilizing membrane proteins, making this buffer suitable for preparing cell lysates for biochemical assays.
-
Nuclear and Cytoplasmic Fractionation: DPBS with NP-40 can be used in protocols to separate nuclear and cytoplasmic fractions of cells.
-
Immunostaining: The buffer is used in immunostaining protocols to permeabilize cells, allowing antibodies to access intracellular targets.
Advantages:
-
The phosphate buffer system in DPBS is designed to resist changes in pH.
-
NP-40 provides a gentle lysis which helps preserve the integrity of proteins and other cellular components.
-
Methods like centrifugal ultrafiltration can be used to remove NP-40 after lysis, ensuring high recovery rates of proteins without significant loss or denaturation.
Composition:
| Ingredients |
Concentration |
| Sodium Chloride |
137 mM |
| Potassium Choride |
2.67 mM |
| Potassium Phosphate monobasic |
1.47 mM |
| Sodium Phosphate dibasic |
8.09 mM |
| NP-40 |
1% |
Quality Control:
-
Appearance: Clear, Colorless Liquid.
-
pH: 7.4
-
Sterility: Filtered 0.1-micron membrane Once and 0.04-micron membrane Once.
Storage and Shelf Life:
-
Storage: 4 ℃
-
Shelf life: 2 years
Safety and Handling:
-
Handle NP-40 with care, as it is a detergent and can be irritating to skin and eyes.
-
Follow appropriate safety protocols, including wearing gloves and eye protection.
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
