Flamma® 749 Hydrazide
Size of product (mg)
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1 mg
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5 mg
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25 mg
Flamma® 749 Hydrazide
Cat. No. List below
Description
The dye exhibits excitation and emission maxima at 749 nm and 774 nm, respectively, positioning it firmly in the NIR region of the spectrum. These spectral characteristics are comparable to other popular NIR dyes such as Alexa 750, Cy7, IRDye 750, and DyLight 755. Flamma 749 can be efficiently excited using a 750 nm laser line or dye-pumped laser, with emission occurring in the NIR region. This spectral range is particularly advantageous for biological tissue imaging due to improved penetration and reduced autofluorescence.
The hydrazide group of Flamma® Fluors 749 Hydrazide reacts with aldehydes and ketones through a reductive amination reaction, forming an imine linkage. This reaction is particularly effective for labeling free reducing sugars on biomolecules and oxidized polysaccharides and glycoproteins (where primary and secondary alcohols have been converted to aldehydes and ketones).
Flamma® Fluors 749 Hydrazide is particularly useful for bioimaging (generates stable fluorescence signals in various biological imaging applications), glycoprotein labeling (enables labeling of glycoproteins after oxidation of sugar moieties), polysaccharide analysis (useful for studying polysaccharide structures and distributions), and aldehyde/ketone detection (can label any biomolecule bearing aldehyde or ketone groups).
For optimal labeling, the following steps are typically involved:
- Oxidation of target biomolecules (if necessary): Convert primary and secondary alcohols on polysaccharides or glycoproteins to aldehydes and ketones
- Reaction with Flamma® Fluors 749 Hydrazide: Allow the hydrazide group to react with the newly formed aldehydes/ketones
- Reduction (optional): The imine linkage can be reduced to form a more stable bond
Flamma® Fluors 749 Hydrazide advantages:
1. Reduced photobleaching compared to visible light fluorophores
2. Lower background fluorescence in biological samples
3. Potential for multiplexing with other fluorophores in different spectral regions
4. Enhanced tissue penetration for in vivo imaging applications
The hydrazide functionality of this dye also opens up possibilities for selective labeling of carbonyl-containing biomolecules, studies on protein glycosylation and carbohydrate metabolism, and development of stimuli-responsive probes (e.g., pH-sensitive fluorescent sensors.
These properties make Flamma® Fluors 749 Hydrazide a valuable tool for researchers in fields such as glycobiology, carbohydrate chemistry, and biomedical imaging, where specific labeling of carbonyl-containing molecules and high-sensitivity detection are crucial. Its unique reactivity expands the toolkit available for bioconjugation and probe development in the NIR spectral range, particularly for studying glycoproteins, polysaccharides, and other carbonyl-bearing biomolecules in complex biological systems.
- Fluorophore: Flamma® Fluors 749
- Reactive group: Hydrazide
- Excitation/Emission Max.(nm): 749/774
- Spectrally similar dyes: Alexa750, DyLight755, Cy7, IRDye750
- Extinction coefficient: ≥ 168,000 cm-1M-1
- Appearance: Green Solid
- Molecular Weight: 724.93 g/mol
- Solubility: DMF, DMSO
- Storage conditions: -20 ℃, protect from light
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| CWH1001 | Flamma® 496 Hydrazide | 496 | 520 | Alexa488, FITC, Cy2 |
| PWH1122 | Flamma® 552 Hydrazide | 550 | 564 | Alexa555, DyLight549, Cy3, ATTO550 |
| KWH1415 | Flamma® 581 Hydrazide | 578 | 593 | Alexa594, DyLight594 |
| PWH1215 | Flamma® 648 Hydrazide | 648 | 663 | Alexa647, DyLight650, Cy5 |
| PWH1515 | Flamma® 675 Hydrazide | 675 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT, CF680 |
| PWH1301 | Flamma® 749 Hydrazide | 749 | 774 | Alexa750, DyLight755, Cy7.5, IRDye750 |
| PWH1603 | Flamma® 774 Hydrazide | 774 | 800 | Cy7.5, DyLight800, IRDye800 |
Background
Flamma® Fluors
BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.
- Covering the full spectral range from UV to NIR
- Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
- High quantum yields and photostability
- High purity and compatible with most of biomolecules
Flamma® Fluors Hydrazide
Flamma® Fluors hydrazide dyes can label aldehyde and ketone through reductive amination reaction to form an imine linkage. The main labeling target for hydrazides are free reducing sugars on biomolecules, and prior to conjugation, primary and secondary alcohols on polysaccharide and glycoprotein are usually oxidized to aldehyde and ketone. Fluorescent modification of aldehyde or carbonyl groups in carbohydrates is also frequently utilized for their analysis by HPLC, capillary electrophoresis and other methods. Hydrazide dyes can also label biomolecules, which introduced aldehyde by genetical or chemical modification.
Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma® Fluors
Figure 2. Immunofluorescence imaging and in situ hybridization imaging
Figure 3. Fluorescence images of Flamma® 749 (upper) and Flamma® 774 (bottom) carboxylic acid injected mouse model
Citation & Reference
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