Protease Inhibitor Cocktail with DPBS Triton-X Buffer

Q: What makes the filtration superior to standard 0.22 micron-filtered buffer?

DCP-PICDPTX1X is filtered through a 0.1 micron membrane twice and a 0.04 micron membrane twice, a quadruple-stage process that removes finer particulates than a single 0.22 micron pass while preventing mycoplasma contamination.

Product Overview

ISO 13485 Certified Manufacturing

FluxMPS™ Protease Inhibitor Cocktail with DPBS Triton-X Buffer

A ready-to-use, MPS-grade combination of a broad-spectrum protease inhibitor cocktail with DPBS Triton X-100 buffer, purpose-built for efficient cell lysis and protein extraction while preserving native protein structure. Quadruple-stage filtered for ultra-clean, sterile performance, it maintains physiological pH and osmolarity for reliable downstream results in Western blotting, immunoprecipitation, and enzymatic assay workflows.

Quadruple-stage filtered: 0.1 µm membrane filtration twice and 0.04 µm membrane filtration twice for ultra-clean, sterile buffer
Broad-spectrum protease inhibitor cocktail combining AEBSF.HCl, Aprotinin, Bestatin, E-64, EDTA, Leupeptin, and Pepstatin A
DPBS base maintains physiological pH and osmolarity during lysis and extraction
Triton X-100 detergent enables efficient cell membrane disruption and protein solubilization
No DNase or RNase activity detected after 18-hour incubation at room temperature
Manufactured under ISO 13485-certified, CE-approved facilities
Microchannel-safe purity suited to microfluidic and organ-on-a-chip (OoC) sample handling
Customizable concentrations, tracking dyes, and pH available on request

Cat. No. DCP-PICDPTX1X | UNSPSC 12161706 | Neutral Buffers

Protease Inhibitor Cocktail with DPBS Triton-X Buffer — 10x10 mL

Catalog No.DCP-PICDPTX1X
Size10x10 mL
FormatReady-to-use liquid
AppearanceClear solution
Filtration0.1 µm x2 + 0.04 µm x2
SterilitySterile-filtered
DNase ActivityNone detected
RNase ActivityNone detected
Storage-80°C
Shelf Life3 months

ISO 13485:2016 USP <85> <785> <788> RUO

Why FluxMPS™

Engineered where standard lysis buffers fail

Conventional 0.22 µm-filtered lysis buffers can carry subvisible particulates, inconsistent detergent dispersion, and variable protease-inhibitor activity into sensitive protein workflows. FluxMPS™ addresses each failure mode with tightly controlled filtration, defined composition, and lot-released quality control.

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Microchannel-safe purity

Quadruple-stage 0.1 µm and 0.04 µm membrane filtration removes particulates that can clog microfluidic channels, valves, and sensors.

target

Balanced, physiological buffer

DPBS base maintains physiological pH and osmolarity so lysed proteins remain in a stable, near-native chemical environment.

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Ultrapure-grade water

Formulated with Ultrapure Type 1 water (18.2 MΩ·cm) to minimize background interference in downstream assays.

visibility

Low background for imaging & assays

Clean, sterile-filtered formulation supports reliable results in Western blotting, immunoprecipitation, and enzymatic assays.

science

Defined, traceable composition

Seven protease inhibitors and buffer salts are each present at a carefully balanced, documented concentration.

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Customization on demand

Alternate concentrations, tracking dyes, and pH modifications are available — contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Every lot of DCP-PICDPTX1X is filtered 0.1 µm membrane twice and 0.04 µm membrane twice, delivering a sterile, ultra-clean buffer suitable for sensitive protein extraction and microfluidic sample handling.

1

0.1 µm Pre-filtration I

Removes large particulates and aggregates, extending the life of downstream filters.
2

0.04 µm Pre-filtration II

Retains fine particulates and bioburden ahead of the final sterile passes.
3

0.1 µm Sterile-filtration I

A second 0.1 µm pass adds redundancy to the sterilization process.
4

0.04 µm Sterile-filtration II — Final Polish

A second 0.04 µm pass provides the final polish, performed in a sterile filling environment.

Performance vs. conventional buffer

Sequential 0.1 µm and 0.04 µm filtration, each applied twice, removes finer particulates than a single 0.22 µm pass, supporting cleaner downstream protein analysis.

0.04 µm

Final filtration stage

4

Total filtration stages

This quadruple-stage filtration process helps prevent mycoplasma contamination; the smallest mycoplasma species measure about 0.2 microns.

DCP-PICDPTX1X FluxMPS Protease Inhibitor Cocktail with DPBS Triton-X Buffer quadruple-stage 0.1 micron and 0.04 micron filtration diagram for organ-on-a-chip and microfluidic protein extraction workflows, Diagnocine — **Figure 1.** Quadruple-stage filtration architecture (0.1 µm membrane filtration twice and 0.04 µm membrane filtration twice) used in production of DCP-PICDPTX1X.
© Diagnocine® — DCP-PICDPTX1X

Applications

Where DCP-PICDPTX1X is used

This ready-to-use protease inhibitor cocktail with DPBS Triton-X buffer supports protein research from bench-scale lysis to microfluidic and organ-on-a-chip sample handling.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra-filtered variant of this buffer is available for automated bioreactor and robotic liquid-handling systems where narrow-bore tubing and sensitive valves demand the lowest achievable particulate load.

Total Particulate Exclusion — supports long-run automated protocols
Valve & Sensor Protection — reduces fouling risk in closed-loop systems
Extended Perfusion Stability — maintains consistent flow in continuous processes

Inquiry Required: Contact support@diagnocine.com to request the 0.01 µm ultra-filtered grade.

Microfluidics

Micro Physiological System (MPS) & Chip

Sterile, low-particulate buffer suited to on-chip protein extraction and lysis steps.

OoCToCBoCLoCMPS

Sample Preparation

Cell Lysis & Protein Extraction

Triton X-100 in DPBS disrupts cell membranes while the protease inhibitor cocktail protects released proteins from degradation.

LysisDilutionReconstitutionRinse

Stem Cell Biology

iPSC-Derived Model Handling

Preserves protein integrity during lysis of iPSC-derived cell models for downstream analysis.

iPSC-NeuronsiPSC-CMiPSC-Hep

Vascular Biology

Endothelial & Primary Cell Perfusion

Supports protein extraction from primary and endothelial cell cultures used in vascular research.

HUVECsHAECsPrimary hepatocytes

Immunoassays

Western Blotting, Immunoprecipitation & Enzymatic Assays

Protease inhibitors preserve protein samples for accurate analysis in Western blotting, immunoprecipitation, and enzymatic assays.

ELISAWestern blotIHCIF

Drug Development & Cell Culture

Protein Target Studies & Tissue Culture

Used in studying protein targets for therapeutic development and in cell/tissue culture work involving cell function and microbial defense investigations.

Drug DevelopmentTissue Culture

Technical Specifications

Detailed specifications

Every value below is drawn directly from this product's release documentation.

Physical & Chemical Parameters

Parameter	Specification
Formulation	Protease inhibitor cocktail in DPBS with Triton X-100 detergent
Appearance	Clear solution
Triton X-100 Concentration	1%
Sodium Chloride Concentration	137 mM
Potassium Chloride Concentration	2.67 mM
Sodium Phosphate dibasic Concentration	8.09 mM
Potassium Phosphate monobasic Concentration	1.47 mM

Sterility, Purity & Safety Parameters

Parameter	Specification
Sterility USP <71>	Filtered 0.1 µm membrane twice and 0.04 µm membrane twice in a sterile environment
DNase Activity	None detected after incubation of plasmid DNA for 18 hours at room temperature
RNase Activity	None detected after incubation of ribosomal RNA for 18 hours at room temperature
Manufacturing Standard ISO 13485	ISO 13485-certified, CE-approved facilities

Storage, Handling & Logistics

Parameter	Specification
Storage Temperature	-80°C
Shelf Life	3 months
Size	10x10 mL

Raw Materials & Regulatory Traceability

Parameter	Specification
Manufacturing QMS ISO 13485	ISO 13485-certified supplier facilities
Regulatory Alignment	CE-approved facilities
Production Method	Final packaging, quality assurance, and testing at the Diagnocine R&D and Quality Testing Center; customization and assembly at Diagnocine Precision, Totowa, New Jersey, USA
Intended Use	Research Use Only (RUO)

Formulation

Full composition

Every protease inhibitor and buffer component is listed below with its release concentration.

Component	CAS Number	Concentration
AEBSF.HCl	30827-99-7	100 µM
Aprotinin	9087-70-1	0.8 µM
Bestatin	58970-76-6	0.05 mM
E-64	66701-25-5	1 µM
EDTA	60-00-4	5 mM
Leupeptin	103476-89-7	0.01 mM
Pepstatin A	26305-03-3	1 µM
Triton-X	9002-93-1	1%
Potassium Chloride	7447-40-7	2.67 mM
Sodium Chloride	7647-14-5	137 mM
Sodium Phosphate dibasic	7558-79-4	8.09 mM
Potassium Phosphate monobasic	7778-77-0	1.47 mM

Other concentrations, additions of tracking dyes, different pH, and other modifications are available on request — contact support@diagnocine.com.

Quality Assurance

Manufacturing & compliance

DCP-PICDPTX1X is manufactured, filtered, and packaged under a controlled quality system.

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ISO 13485:2016 QMS

Manufactured under ISO 13485-certified and CE-approved supplier facilities.

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Ultrapure Type 1 Water

Formulated with Ultrapure Type 1 water (18.2 MΩ·cm).

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Sterile Fill & Finish

Filtered 0.1 µm membrane twice and 0.04 µm membrane twice in a sterile environment.

assignment

Micro-Batch Precision

Final packaging, QA, and testing performed at the Diagnocine R&D and Quality Testing Center; customization and assembly at Diagnocine Precision, Totowa, New Jersey, USA.

DNase Activity

None detected after incubation of plasmid DNA for 18 hours at room temperature.

RNase Activity

None detected after incubation of ribosomal RNA for 18 hours at room temperature.

Appearance

Clear solution.

Documentation

A Certificate of Analysis is available for this lot.

Request a Certificate of Analysis at support@diagnocine.com.

Product Comparison

How DCP-PICDPTX1X compares

A side-by-side look at filtration architecture and quality attributes versus conventional lysis buffer preparations.

Parameter	DCP-PICDPTX1X (FluxMPS™)	Conventional Buffer	Standard Alternative
Protease inhibitor cocktail included	check_circle	cancel	cancel
Final filtration pore size	0.04 µm	0.22 µm	0.22 µm
Number of filtration stages	4	1	1
DNase/RNase activity verified	check_circle	cancel	cancel
Water quality	Ultrapure Type 1 (18.2 MΩ·cm)	Not specified	Not specified
Manufacturing QMS	ISO 13485-certified	Not specified	Not specified
Microfluidic channel compatibility	check_circle	cancel	cancel
Physiological pH/osmolarity buffer base	check_circle	check_circle	cancel
Custom formulation available	check_circle	cancel	cancel

FAQ

Frequently asked questions

Answers to common questions about DCP-PICDPTX1X.

Yes. Its quadruple-stage, ultra-clean filtration and sterile-filtered, low-particulate formulation make it well suited to microfluidic channels and organ-on-a-chip sample handling.

DCP-PICDPTX1X is filtered through a 0.1 µm membrane twice and a 0.04 µm membrane twice, a quadruple-stage process that removes finer particulates than a single 0.22 µm pass while preventing mycoplasma contamination.

The DPBS base maintains a physiological pH and osmolarity. Alternate concentrations, different pH, and other modifications are available on request — contact support@diagnocine.com.

DNase and RNase activity were verified after 18-hour incubations at room temperature, with none detected. For long-term storage, keep the product at -80°C; shelf life is 3 months.

Yes. Other concentrations, additions of tracking dyes, different pH, and other modifications can be requested at support@diagnocine.com.

Endotoxin testing is not part of this product's standard release documentation. Contact support@diagnocine.com for the current Certificate of Analysis and available testing options.

Yes. A Certificate of Analysis covering appearance, sterility, DNase activity, and RNase activity is available — request it at support@diagnocine.com.

Scientific References

Supporting literature

Curated literature relevant to protease inhibition, protein extraction, and microfluidic sample handling.

Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970;227:680-685. doi:10.1038/227680a0
Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72:248-254. doi:10.1016/0003-2697(76)90527-3
Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA. 1979;76:4350-4354. doi:10.1073/pnas.76.9.4350
Mahmood T, Yang PC. Western blot: technique, theory, and trouble shooting. N Am J Med Sci. 2012;4:429-434. doi:10.4103/1947-2714.100998
Huh D, Hamilton GA, Ingber DE. From 3D cell culture to organs-on-chips. Trends Cell Biol. 2011;21:745-754. doi:10.1016/j.tcb.2011.09.005
Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760-772. doi:10.1038/nbt.2989
Drucker E, Krapfenbauer K. Pitfalls and limitations in translation from biomarker discovery to clinical utility in predictive and personalised medicine. EPMA J. 2013;4:7. doi:10.1186/1878-5085-4-7
Malm J, Fehniger TE, Danmyr P, et al. Developments in biobanking workflow standardization providing sample integrity and stability. J Proteomics. 2013;95:38-45. doi:10.1016/j.jprot.2012.10.030