.jpg "/Content/Upload/Product/Human_ELISA_kits_Diagnocine_1200_1200(78).jpg")
Human Transforming Growth factor β1,TGF-β1 ELISA kit
The Ultimate 1-Stop ELISA Marketplace:
60,000+ ELISA kits Available to Serve Scientists from All Fields of Life Science
Always Powered by Diagnocine for Precision Research.
Most comprehensive buffers, reagents and detection agents are provided with each kit to optimize data output.
All-inclusive format covers everything from sample preparation to signal detection. Every step can be customized and optimized for your precision research.
DiagnoCine Precision buffers and reagents are Made in USA ISO 13485 Certified
Why waste time searching for your ELISA kit when you can get everything in one place? Diagnocine can provide you with all the ELISA needs you have (customized ELISAs also available) on the planet through a single, streamlined ordering process.
* all types of species
* HRP and AP detection
* Low target signal enhancement
* Inquire about a trial version or a customized version
4 core reagents below are included with every order, plus 1 of the 2 detection-specific reagents (matched automatically to your kit type) — 5 components, every time.
+ the 5th component, matched to your kit's detection method:
If you have any questions or need additional information, please call us at 551-245-1033 or email us at support@diagnocine.com. Our team will be happy to assist you.
Universal Compatibility
All buffers and detection reagents work seamlessly across target proteins, bridging the gap in assay consistency.
1-Stop Convenience
Consolidate your lab's purchasing. Source all ELISA needs and experience optimal buffers and reagents in a single shipment.
Enhanced Precision
Designed by scientists for scientists, all components are engineered to deliver sharper results and an optical signal.
Human Transforming Growth factor β1,TGF-β1 ELISA kit
Sandwich quantitative immunoassay for transforming growth factor, beta 1 in human serum, plasma, cell culture supernates available in multiple catalog sizes:
Note: Please send inquiries regarding Trial 24T orders to support@diagnocine.com.
| Target name | transforming growth factor, beta 1 |
| Uniprot No. | P01137 |
| Species | Homo sapiens (Human) |
| Sample types | serum, plasma, cell culture supernates |
| Detection range | 0.78 ng/mL-50 ng/mL |
| Sensitivity | 0.747 ng/mL |
| Assay time | 1-5h |
| Sample loading volume | 50-100μL |
| Detection wavelength | 450 nm |
| Assay principle | Sandwich (Quantitative) |
| Data analysis | Standard curve + Curve Expert software |
| Research area | Signal Transduction |
| Storage condition | 2-8°C (see protocol for full details) |
| Shipping condition | 4 °C |
| Shelf life | 6 months |
10 business days
Processing + 3-5 days shipping
In Stock : USA
Worldwide shipping available
Antibody capture
450 nm detection
Standard curve
Quantification
In this sandwich ELISA, transforming growth factor, beta 1 in the sample is captured between a pre-coated capture antibody and a detection antibody. Signal intensity is proportional to analyte concentration. Quantification uses a standard curve fitted with Curve Expert software, covering 0.78 ng/mL-50 ng/mL with a minimum detectable dose of 0.747 ng/mL.
The product CSB-E04725h is a ready-to-use microwave, strip plate ELISA kit designed to quantitatively analyze the TGFB1 in different biological samples, including serum, plasma, and cell culture supernates. This TGFB1 ELISA kit specifically recognizes the human TGFB1 in the sample. It adopts the Sandwich ELISA technique in conjunction with an enzyme colorimetric reaction to indicate the presence of human TGFB1 and quantitatively measures the level of TGFB1 in the samples. The intensity of the colored product is positively related to the amount of human TGFB1 in the sample.
Human TGFB1 is the most abundant TGFB isoform and was cloned from the human placenta. It is a pleiotropic growth factor that exerts multiple effects on inflammation, angiogenesis, fibrosis, tissue repair, and tumor progression. TGFB1 also exhibits various anti-inflammatory activities and contributes to immune homeostasis and inhibition of autoimmunity. TGFB1 acting as a very potent stimulator of chemotaxis stimulates monocyte, lymphocyte, neutrophil, and fibroblast migration. Dysregulated TGFB1 signaling has been associated with cellular proliferation, cancer development, and metastasis. TGFB1 upregulation mediated promotion of the epithelial-mesenchymal transition (EMT) enhances invasiveness, metastases development, angiogenesis, and immune suppression.
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
To assess the linearity of the assay, samples were spiked with high concentrations of human TGF-β1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
