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Flamma® 648 Vinylsulfone

Product#: PWA1215

$765.92

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1 mg
5 mg
25 mg

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Flamma® 648 Vinylsulfone

Cat. No. List below

Description

Flamma® Fluors 648 Vinylsulfone is a state-of-the-art, pH-insensitive red fluorescent dye engineered for exceptional stability and performance in bioimaging applications. Developed using BioActs' leading technology, this innovative dye represents a significant advancement in fluorescent labeling techniques.

Key Features:
1. pH-insensitive formulation stable across a wide pH range (5 to 10)
2. High-temperature resistance
3. Unique vinylsulfone reactive group
4. Excitation/Emission maxima at 648/663 nm
5. High sensitivity for low-abundance biomolecule detection
6. Superior dye-to-protein (d/P) ratio for enhanced signal strength
7. Excellent photostability
8. Strong absorption and high fluorescence quantum yie

Applications:
1. Fluorescence microscopy and confocal imaging
2. Flow cytometry
3. In vivo imaging
4. Protein and nucleic acid labeling
5. Cellular tracking and detection
6. Harsh environment studies
7. Labeling of antibodies, peptides, proteins, and ligands
8. Amplification substrates for enhanced sensitivity
9. Single-molecule detection
10. High-content screening (HCS)
11. High-throughput screening (HTS)

Advantages:
1. Versatile conjugation: Readily reacts with primary amines of amino-modified oligonucleotides or proteins
2. Stable linkage: Forms a robust amino linkage between the dye and biomolecules
3. Consistent performance: Reliable results across a wide range of experimental conditions
4. Spectral similarity: Comparable to Alexa 647, DyLight 650, and Cy5, allowing for easy integration into existing protocols
5. Superior brightness: Maintains excellent fluorescence after conjugation
6. Reduced background: Lower non-specific binding for improved signal-to-noise ratios
7. Long-lasting labeling: Ensures reliable results even under challenging conditions
8. Compatibility: Works well with most fluorescent equipment and optical conditions

Flamma® Fluors 648 Vinylsulfone is part of the broader Flamma® Fluors series, which covers a wide spectral range from UV to NIR. These dyes are characterized by strong absorption, high fluorescence quantum yield, and excellent photostability. They maintain good fluorescence activity and stability after conjugation to biomolecules, allowing for the detection of low-abundance biological structures with great sensitivity.

The vinylsulfone reactive group offers unique advantages, including stability in organic and aqueous solutions, making it versatile for various experimental setups. This feature, combined with its pH insensitivity and high-temperature stability, makes Flamma® 648 Vinylsulfone an excellent choice for researchers working on challenging detection problems or in harsh experimental conditions.

Researchers can leverage Flamma® 648 Vinylsulfone for a variety of fluorescence-based techniques in life science research and medical diagnostics. Its superior optical properties, stability, and versatility make it a valuable tool for cutting-edge biological studies, drug discovery, toxicity testing, and immunology research.

Specifications

Fluorophore: Flamma® Fluors 648
Reactive group: Vinylsulfone
Excitation/Emission Max.(nm): 648/663
Spectrally similar dyes: Alexa647, DyLight650, Cy5
Extinction coefficient: ≥ 227,000 cm-1M-1
CF280: 0.03
Appearance: Blue Solid
Molecular Weight: 802.03 g/mol
Solubility: DMF, DMSO
Storage conditions: 4 ℃, protect from light

Quick link (Cat.#)	FSD series	EXmax (nm)	EMmax (nm)	Spectrally similar dyes
CWA1002	Flamma® 488 Vinylsulfone	495	519	Alexa488, Cy2
CWA1001	Flamma® 496 Vinylsulfone	496	520	Alexa488, FAM
PWA1122	Flamma® 552 Vinylsulfone	550	564	Alexa555, DyLight549, Cy3, ATTO550, CF555
PWA1415	Flamma® 581 Vinylsulfone	581	596	Alexa594, DyLight594
KOA1001	Flamma® 594 Vinylsulfone	590	617	Alexa594, DyLight594
PWA1215	Flamma® 648 Vinylsulfone	648	683	Alexa647, DyLight650, Cy5
PWA1515	Flamma® 675 Vinylsulfone	675	691	Alexa680, DyLight680, Cy5.5, IRDye680LT, CF680
PWA1308	Flamma® 749 Vinylsulfone	749	774	Alexa750, DyLight755, Cy7, IRDye750
PWA1603	Flamma® 774 Vinylsulfone	774	795	Cy7.5, CF 770, DyLight 800, IRDye800
POA1803	Flamma® 800 Vinylsulfone	774	789	Cy7.5, DyLight800, IRDye800

Background

Flamma® Fluors

BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.

Covering the full spectral range from UV to NIR
Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide
and hydrophobic substances.
High quantum yields and photostability
High purity and compatible with most of biomolecules

Why Choose Flamma® Fluors 648 Vinylsulfone?

pH Insensitivity: Maintains stability and performance across various pH levels
Temperature Resistance: Ideal for high-temperature applications
Versatility: Compatible with a wide range of biomolecules and experimental conditions
Superior Brightness: High extinction coefficient for enhanced sensitivity
Stability: Generates stable fluorescence signals for reliable results

Comparison with Other Dyes:

Feature	Flamma® Fluors 648 Vinylsulfone	Traditional Cyanine Dyes
pH Sensitivity	Insensitive	Sensitive
Temperature Stability	High	Variable
Reactive Group	Vinylsulfone	Various
Labeling Stability	Excellent	Good

Flamma^® Fluors Vinylsulfone

BioActs’ leading technology developed highly reactive yet stable and pH insensitive Flamma® Fluors Vinylsulfone dyes. Vinylsulfone reactive group mainly react with the primary amine of biomolecules under a wide range of pH from 5 to 10 though the labeling reaction rate is proportional to the basicity of medium. Vinylsulfone dyes are stable in both organic and aqueous solution at higher temperature, thus they can be dissolved in water avoiding organic solvents such as DMSO or DMF, and the aqueous stock solution can be stored in a refrigerator. Vinylsulfone reacting group is added by an amine via 1,2 addition pathway to form a stable amino linkage between dye and the biomolecule, therefore none of side product would be generated during conjugation step. BioActs recommends Flamma® Fluors Vinylsulfone for labeling biomolecules in harsh conditions such as having less reactive amines, running the reaction in prolong period, at high temperature or under strong basic or acidic environment.

Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma^® Fluors

Figure 2. Immunofluorescence imaging and in situ hybridization imaging

Figure 3. Fluorescence images of Flamma^® 749 (upper) and Flamma^®774 (bottom) carboxylic acid injected mouse model

Citation & Reference

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