TET NA NHS ESTER
Size of product (mg)
-
1 mg
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5 mg
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25 mg
TET NA NHS ESTER
Cat. No. List below
Description
TET NA NHS ester is an amine-reactive fluorescent dye used for generating stable fluorescence signals in bioimaging applications. This versatile dye is analogous to tetramethylrhodamine (TAMRA) and can be used as a replacement for similar dyes in various molecular biology and biochemistry research applications.
Key Features:
1. Excitation/Emission maxima: Approximately 555/580 nm (orange-red region)
2. Amine-reactive NHS ester group for efficient labeling
3. Stable fluorescence signal
4. Compatible with various biomolecules
Applications:
1. Protein and peptide labeling
2. Amino-modified oligonucleotide labeling
3. Fluorescence microscopy
4. FRET (Förster Resonance Energy Transfer) experiments
5. In vitro and in vivo imaging
6. Flow cytometry
Advantages:
1. Forms chemically stable amide bonds with target molecules
2. Versatile labeling of various biomolecules
3. Suitable for solid-phase oligonucleotide synthesis
4. Can be used as a substitute for several spectrally similar dyes
5. Enables multicolor detection in combination with other fluorophores
6. High sensitivity for detecting low-abundance biomolecules
7. Optimal pH range for labeling (8.3-8.5) allows for efficient conjugation
HEX NA NHS ester readily reacts with amino groups of proteins and modified nucleotides, forming stable amide bonds between the dye and the target biomolecule. This property makes it an excellent choice for various labeling applications in molecular biology and biochemistry research.
Specifications
- Appearance: Orange-red fluorescent dye
- Molecular weight: Approximately 650-700 g/mol (estimated)
- Solubility: Soluble in organic solvents (e.g., DMF, DMSO)
- Storage: -20°C, protected from light
- Reactive group: NHS ester
- Target groups: Primary amines (e.g., ε-amino groups of lysine, N-terminus of proteins)
- Spectral characteristics: Similar to TAMRA and other rhodamine derivatives
- Optimal labeling pH: 8.3-8.5
TET NA NHS ester readily reacts with amino groups of proteins, peptides, and modified nucleotides, forming stable amide bonds between the dye and the target biomolecule1. This property makes it an excellent choice for various labeling applications in molecular biology, biochemistry, and bioimaging research. The dye's spectral properties in the orange-red region allow for good tissue penetration and reduced autofluorescence, making it suitable for both in vitro and in vivo imaging applications.
| Quick link (Cat.#) | Series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| PNS1122 | Flamma® 552NA NHS ester | 553 | 570 | Alexa 555, DyLight 549, Cy 3, ATTO 550 |
| PNS1415 | Flamma® 581NA NHS ester | 590 | 608 | Alexa 594, DyLight 594, Cy 3.5 |
| KNS1001 | Flamma® 594NA NHS ester | 583 | 603 | Alexa594, DyLight594, Texas Red-X |
| PNS1215 | Flamma® 648NA NHS ester | 646 | 665 | Alexa647, DyLight650, Cy5 |
| PNS1515 | Flamma® 675NA NHS ester | 689 | 709 | Alexa680, DyLight680, Cy5.5, IRDye680LT, CF 680 |
| COS1030 | HEX NA NHS ester | |||
| COS1022 | TET NA NHS ESTER |
Background
Fluorescent Dyes for Oligonucleotide Synthesis
Fluorescent oligonucleotide probes are developed for the detection and the quantification of target DNA or RNA sequence with the high sensitivity and specificity along with low toxicity. They have been used in a wide range of applications including, FISH, in situ hybridization, sequencing and genotyping. Fluorescent dye and quencher paired molecular beacon probes are widely used in the field of real–time nucleic acid detection and PCR quantification, SNP detection, and clinical diagnostic assays.
There are two major strategies for chemical incorporation of fluorescent dyes into an oligonucleotides: direct fluorescence labeling during chemical synthesis and post synthetic fluorescence labeling. The direct oligonucleotide labeling during solid phase synthesis can be achieved via the standard phosphoramidite chemistry containing various spacer arm lengths. Direct labeling during solid phase chemical synthesis might afford high yield but also increases the risk of damaging fluorescence tag from acidic deprotection step. Thus certain fluorescent dyes are not compatible with solid phase chemical synthesis, thus their labeling must be conducted post–synthetically through various labeling methodologies.
Post synthetic labeling of a fluorescent dye to an oligonucleotide can be achieved by labeling various activated dyes to modified oligomers having functional groups such as primary amine, thiol, aldehyde, azide, alkyne, or carboxylic acids. Depending on binding target, the fluorophore might be selectively introduced at either the 5′ or 3′ ends of the oligonucleotide as well as randomly incorporated throughout the sequence. BioActs offers fluorescent dye phosphoramidites and fluorescent nucleotide phosphoramidites for direct fluorescence labeling and Flamma NA series dyes for post synthetic fluorescence labeling method.
Citation & Reference
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