Flamma® 749 Amine
Size of product (mg)
-
1 mg
-
5 mg
-
25 mg
Flamma® 749 Amine
Cat. No. List below
Description
Key features:
1. Excitation/Emission maxima: 749/774 nm
2. Primary amine functional group attached through a spacer
3. Spectral similarity to popular NIR dyes (Alexa 750, Cy7, IRDye 750, DyLight 755)
4. Soluble in DMF and DMSO
Applications:
1. Bioimaging and fluorescence microscopy
2. In vitro and in vivo imaging studies
3. Coupling with carboxylic acids on small molecules or biomolecules
4. Reference standard for dye-conjugates
5. Deep-tissue and whole-body imaging
6. Potential use in fluorescence-guided surgery
7. Protein labeling and antibody conjugation
8. Nucleic acid tagging
9. Nanoparticle functionalization
Advantages:
1. Enhanced tissue penetration due to NIR spectral properties
2. Reduced autofluorescence and light scattering in biological samples
3. Compatibility with 750 nm laser line or dye-pumped laser excitation
4. Versatile coupling options through standard amide bond formation
5. Stable fluorescence signal for extended imaging sessions
6. High sensitivity for detecting low-abundance biological structures
7. Potential for multiplexing with other fluorophores in different spectral regions
8. Reduced phototoxicity for biological components
- Fluorophore: Flamma® Fluors 749
- Functional group: Primary amine
- Excitation/Emission Max.(nm): 749/774
- Spectrally similar dyes: Alexa750, DyLight755, Cy7, IRDye750
- Extinction coefficient: ≥ 200,000 cm-1M-1
- CF280: 0.03
- Appearance: Green Solid
- Molecular Weight: 752.98 g/mol
- Solubility: DMF, DMSO
- Storage conditions: 4 ℃, protect from light
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| CWE1001 | Flamma® 496 Amine | 496 | 520 | Alexa488, FITC, Cy2 |
| PWE1122 | Flamma® 552 Amine | 550 | 565 | Alexa555, DyLight549, Cy3, ATTO550 |
| KWE1415 | Flamma® 581 Amine | 578 | 593 | Alexa594, DyLight594 |
| PWE1215 | Flamma® 648 Amine | 648 | 663 | Alexa647, DyLight650, Cy5 |
| PWE1515 | Flamma® 675 Amine | 675 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT, CF680 |
| PWE1301 | Flamma® 749 Amine | 749 | 774 | Alexa750, DyLight755, Cy7.5, IRDye750 |
| PWE1603 | Flamma® 774 Amine | 774 | 800 | Cy7.5, DyLight800, IRDye800 |
Background
Flamma® Fluors
BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.
- Covering the full spectral range from UV to NIR
- Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
- High quantum yields and photostability
- High purity and compatible with most of biomolecules
1. Reduced photobleaching compared to visible light fluorophores
2. Lower background fluorescence in biological samples
3. Potential for multiplexing with other fluorophores in different spectral regions
These properties make Flamma® Fluors 749 Amine a valuable tool for researchers in fields such as molecular biology, biochemistry, and biomedical imaging, where high-sensitivity detection and low background interference are crucial.
Flamma® Fluors Amine
Flamma® Fluors Amine dyes have an attached primary amine, which connected through a spacer, and can be used as a reference standard for dye-conjugates. These amine dyes can be conjugated with carboxylic acids at small molecules or peptides by standard amide bond coupling conditions.
Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma® Fluors
Figure 2. Immunofluorescence imaging and in situ hybridization imaging
Figure 3. Fluorescence images of Flamma® 749 (upper) and Flamma® 774 (bottom) carboxylic acid injected mouse model
Citation & Reference
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