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Flamma® 774 Vinylsulfone

Product#: PWA1603

$967.52

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5 mg
25 mg

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Flamma® 774 Vinylsulfone

Cat. No. List below

Description

Flamma® Fluors 774 Vinylsulfone is an advanced near-infrared (NIR) fluorescent dye designed for high-performance bioimaging and fluorescence detection applications. This pH-insensitive reactive dye offers exceptional signal-to-noise ratios and stable fluorescence signals, making it ideal for a wide range of biological labeling techniques.

The chemical properties are its reactive groups is vinylsulfone. It is stable in a wide range of pH from 5 to 10. It is stable in both organic and aqueous solutions and maintains stability at high temperatures.

The vinylsulfone group readily reacts with primary amines through a 1,2-addition mechanism, including amino groups of protein and amino-modified oligonucleotides. This reaction forms a stable amino linkage between the dye and the target biomolecule, ensuring robust and long-lasting labeling.

Flamma® Fluors 774 Vinylsulfone is particularly suited for protein labeling, antibody conjugation, nucleic acid labeling, in vitro imaging, in vivo NIR imaging, peptide & ligand labeling, and other fluorescence detection methods.

Flamma® Fluors 774 Vinylsulfone represents a powerful tool for researchers in life sciences, offering superior NIR fluorescence properties for advanced imaging and detection applications. Its pH insensitivity, high stability, and versatile reactivity make it an excellent choice for a wide range of biological research and diagnostic applications, including challenging in vivo NIR imaging studies.

What are the advantages?
1. pH Insensitivity: Maintains stability and reactivity across a wide pH range, offering flexibility in labeling conditions.
2. High Signal-to-Noise Ratio: Provides clear and distinct signals, crucial for sensitive detection applications.
3. NIR Fluorescence: Emits in the NIR region, allowing for deep tissue imaging with minimal autofluorescence background.
4. Stable Conjugation: Forms a stable amino linkage with biomolecules, ensuring long-lasting labeling.
5. Versatility: Suitable for labeling various biomolecules, including proteins, antibodies, peptides, and nucleic acids.
6. Photostability: Exhibits high photostability, allowing for extended imaging sessions.

Specifications

Fluorophore: Flamma® Fluors 774
Reactive group: Vinylsulfone
Excitation/Emission Max.(nm): 774/795
Spectrally similar dyes: Cy7.5, CF 770, DyLight 800, IRDye800
Extinction coefficient: ≥ 144,000 cm-1M-1
CF280: 0.1
Appearance: Green Solid
Molecular Weight: 1046.23 g/mol
Solubility: DMF
Storage conditions: 4 ℃, protect from light

Quick link (Cat.#)	FSD series	EXmax (nm)	EMmax (nm)	Spectrally similar dyes
CWA1002	Flamma® 488 Vinylsulfone	495	519	Alexa488, Cy2
CWA1001	Flamma® 496 Vinylsulfone	496	520	Alexa488, FAM
PWA1122	Flamma® 552 Vinylsulfone	550	564	Alexa555, DyLight549, Cy3, ATTO550, CF555
PWA1415	Flamma® 581 Vinylsulfone	581	596	Alexa594, DyLight594
KOA1001	Flamma® 594 Vinylsulfone	590	617	Alexa594, DyLight594
PWA1215	Flamma® 648 Vinylsulfone	648	683	Alexa647, DyLight650, Cy5
PWA1515	Flamma® 675 Vinylsulfone	675	691	Alexa680, DyLight680, Cy5.5, IRDye680LT, CF680
PWA1308	Flamma® 749 Vinylsulfone	749	774	Alexa750, DyLight755, Cy7, IRDye750
PWA1603	Flamma® 774 Vinylsulfone	774	795	Cy7.5, CF 770, DyLight 800, IRDye800
POA1803	Flamma® 800 Vinylsulfone	774	789	Cy7.5, DyLight800, IRDye800

Background

Flamma® Fluors

BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.

Covering the full spectral range from UV to NIR
Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide
and hydrophobic substances.
High quantum yields and photostability
High purity and compatible with most of biomolecules

Flamma^® Fluors Vinylsulfone

BioActs’ leading technology developed highly reactive yet stable and pH insensitive Flamma® Fluors Vinylsulfone dyes. Vinylsulfone reactive group mainly react with the primary amine of biomolecules under a wide range of pH from 5 to 10 though the labeling reaction rate is proportional to the basicity of medium. Vinylsulfone dyes are stable in both organic and aqueous solution at higher temperature, thus they can be dissolved in water avoiding organic solvents such as DMSO or DMF, and the aqueous stock solution can be stored in a refrigerator. Vinylsulfone reacting group is added by an amine via 1,2 addition pathway to form a stable amino linkage between dye and the biomolecule, therefore none of side product would be generated during conjugation step. BioActs recommends Flamma® Fluors Vinylsulfone for labeling biomolecules in harsh conditions such as having less reactive amines, running the reaction in prolong period, at high temperature or under strong basic or acidic environment.

Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma^® Fluors

Figure 2. Immunofluorescence imaging and in situ hybridization imaging

Figure 3. Fluorescence images of Flamma^® 749 (upper) and Flamma^®774 (bottom) carboxylic acid injected mouse model

Citation & Reference

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