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Flamma® 675 Vinylsulfone

Product#: PWA1515

$967.52

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25 mg

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Flamma® 675 Vinylsulfone

Cat. No. List below

Description

Flamma® Fluors 675 Vinylsulfone is a cutting-edge near-infrared (NIR) fluorescent dye designed for superior performance in bioimaging applications. This pH-insensitive reactive dye, derived from benzindocyanine structure, offers exceptional stability and versatility across a wide range of experimental conditions.

Key Features:

pH Insensitivity: Maintains stability across a broad pH range
Temperature Stability: Performs reliably at high temperatures
Reactive Group: Features a vinylsulfone group for efficient biomolecule labeling
Spectral Properties: Ex/Em maxima at 675/691 nm, comparable to popular dyes like Alexa 680 and Cy5.5
NIR Emission: Ideal for deep tissue imaging with minimal background interference
Versatile Applications: Suitable for labeling antibodies, peptides, proteins, ligands, and amplification substrates

Flamma® Fluors 675 Vinylsulfone excels in various bioimaging applications, including, In Vitro Imaging (optimized for high-resolution cellular imaging), protein labeling (efficiently tags antibodies and other proteins),nucleic acid studies (suitable for labeling amino-modified oligonucleotides, and ligand binding assays (ideal for fluorescence-based binding studies). We offer Flamma Fluors Vinylsulfone 675 for labeling of antibodies, peptides, proteins, ligands and amplification substrates optimized for in vitro imaging.

Flamma® Fluors 675 Vinylsulfone represents a significant advancement in NIR fluorescent dyes for bioimaging. Its unique combination of stability, reactivity, and spectral properties makes it an excellent choice for researchers seeking high-quality, reliable results in their fluorescence-based experiments. Whether you're conducting in vitro imaging, protein labeling, or exploring complex biological systems, this dye offers the performance and versatility needed to push the boundaries of your research.

Specifications

Fluorophore: Flamma® Fluors 675
Reactive group: Vinylsulfone
Excitation/Emission Max.(nm): 675/691
Spectrally similar dyes: Alexa680, DyLight680, Cy5.5, IRDye680LT, CF680
Extinction coefficient: ≥ 200,000 cm-1M-1
CF280: 0.09
Appearance: Blue Solid
Molecular Weight: 1062.28 g/mol
Solubility: DMF, DMSO
Storage conditions: 4 ℃, protect from light

Quick link (Cat.#)	FSD series	EXmax (nm)	EMmax (nm)	Spectrally similar dyes
CWA1002	Flamma® 488 Vinylsulfone	495	519	Alexa488, Cy2
CWA1001	Flamma® 496 Vinylsulfone	496	520	Alexa488, FAM
PWA1122	Flamma® 552 Vinylsulfone	550	564	Alexa555, DyLight549, Cy3, ATTO550, CF555
PWA1415	Flamma® 581 Vinylsulfone	581	596	Alexa594, DyLight594
KOA1001	Flamma® 594 Vinylsulfone	590	617	Alexa594, DyLight594
PWA1215	Flamma® 648 Vinylsulfone	648	683	Alexa647, DyLight650, Cy5
PWA1515	Flamma® 675 Vinylsulfone	675	691	Alexa680, DyLight680, Cy5.5, IRDye680LT, CF680
PWA1308	Flamma® 749 Vinylsulfone	749	774	Alexa750, DyLight755, Cy7, IRDye750
PWA1603	Flamma® 774 Vinylsulfone	774	795	Cy7.5, CF 770, DyLight 800, IRDye800
POA1803	Flamma® 800 Vinylsulfone	774	789	Cy7.5, DyLight800, IRDye800

Background

Flamma® Fluors

BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.

Covering the full spectral range from UV to NIR
Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide
and hydrophobic substances.
High quantum yields and photostability
High purity and compatible with most of biomolecules

What are the advantages?

Superior Stability: The vinylsulfone reactive group, developed through BioActs' leading technology, ensures exceptional stability across diverse experimental conditions.
Efficient Coupling: Readily reacts with primary amines, forming stable linkages between the dye and biomolecules.
Spectral Compatibility: Similar spectral properties to widely used dyes like Alexa 680, Cy5.5, IRDye 680LT, and DyLight 680, allowing for seamless integration into existing imaging setups.
Deep Tissue Imaging: NIR emission enables imaging of deeper tissues with reduced autofluorescence and improved signal-to-noise ratio.
Versatile Excitation: Can be excited using a 633 nm laser line, compatible with many common fluorescence microscopy systems.

Flamma^® Fluors Vinylsulfone

BioActs’ leading technology developed highly reactive yet stable and pH insensitive Flamma® Fluors Vinylsulfone dyes. Vinylsulfone reactive group mainly react with the primary amine of biomolecules under a wide range of pH from 5 to 10 though the labeling reaction rate is proportional to the basicity of medium. Vinylsulfone dyes are stable in both organic and aqueous solution at higher temperature, thus they can be dissolved in water avoiding organic solvents such as DMSO or DMF, and the aqueous stock solution can be stored in a refrigerator. Vinylsulfone reacting group is added by an amine via 1,2 addition pathway to form a stable amino linkage between dye and the biomolecule, therefore none of side product would be generated during conjugation step. BioActs recommends Flamma® Fluors Vinylsulfone for labeling biomolecules in harsh conditions such as having less reactive amines, running the reaction in prolong period, at high temperature or under strong basic or acidic environment.

Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma^® Fluors

Figure 2. Immunofluorescence imaging and in situ hybridization imaging

Figure 3. Fluorescence images of Flamma^® 749 (upper) and Flamma^®774 (bottom) carboxylic acid injected mouse model

Citation & Reference

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