Flamma® 648 Sulfo-NHS ester

• Covering the full spectral range from UV to NIR
• Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
• High quantum yields and photostability
• High purity and compatible with most of biomolecules

• Superior Water Solubility: Ideal for aqueous-based experiments and live-cell imaging
• Brightness: High extinction coefficient for enhanced sensitivity
• Stability: Generates stable fluorescence signals for reliable results
• Versatility: Compatible with various biomolecules and applications
• Ease of Use: No need for organic co-solvents, simplifying experimental procedures

Flamma^® Fluors NHS ester / Sulfo-NHS ester

Succinimidyl (NHS) esters are reliable activating moiety for amine labeling due to the formation of the strong amide bond. NHS esters are generally stable when storing in anhydrous and the dark condition at -20. NHS ester dyes display good reactivity toward aliphatic amines yet low reactivity with aromatic amines, alcohols, phenols and histidine. The basicity of an amine is an important factor for its reactivity: virtually all proteins have lysine residues, and most of them have a free N-terminus amine. The reactivity of ε-amino group at lysine is pH dependent that the optimal pH for labeling lysine residue is 8.5~9.5. However, the primary amine at N-terminus usually has a pKa value of ~7, thus it can be selectively conjugated by running the reaction in neutral pH. Due to the solubility issue, Flamma^® Fluors NHS esters are required to be dissolved in an organic solvent such as DMSO or DMF and added to aqueous solution. To overcome this limitation, BioActs offers water soluble Flamma^® Fluors Sulfo-NHS ester dyes, which can eliminate the usage of organic solvents in the conjugation reaction. NHS and Sulfo-NHS ester dyes are unstable under humid condition, thus they should be in a lyophilized form or a solution in the anhydrous organic solvent such as DMF or DMSO and stored at -20 ℃.
 

Figure 1.   Absorption (upper) and emission (bottom) spectra overlap of Flamma^® Fluors

Figure 2.   Immunofluorescence imaging and in situ hybridization imaging

Figure 3.   Fluorescence images of Flamma^® 749 (upper) and Flamma^® 774 (bottom) carboxylic acid injected mouse

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