Anti-HBcAg Antibody, Poly

Cat. No.: FNK-BCL-ABPC-01

Size: 100μg

Storage: 4℃ (for long term storage, -20℃. Avoid freeze and thaw cycles)


Introduction:

HBsAg is the surface antigen of Hepatitis B virus (HBV). HBsAg is known to be present in blood if HBV infected patients, and used as a important diagnostic maker of HBV infection. HBsAg forms a small lipid particle（diameter 50 to 60nm）in which many antigen protein are present. Each antigen protein has three domains, called S, Pre-S1 and Pre-S2. L-type antigen contains all the three domains, M-type antigen contains S- and Pre-S2, and S-type antigen consisted of only S-domains. During the process of HBV infection, Pre-S1 domain plays a key role for host (hepatic) cell recognition, and Pre-S2 domain is essential for cell penetration. In infected patients, hepatic cells produces a lot of non-infectious S-type HBsAg (HBV mimetics without genome). The detection of serum Pre-S2 and Pre-S1 antigen is, therefore, very important to reveal the mechanism of HBV infection.




Hepatitis B virus has core structure in which virus genome is contained. C proteins, by selfassembling each other, form the core (or capsid) structure (a hollow nanoparticle) . Virus genome (DNA) is encapsulated in the nanoparticle. Thus, HBcAg is a particle composed of C proteins. HBcAg is not generally found in infected serum, but antibody against HBcAg is a diagnostic marker that indicate history of HBV infection. This product is an antiobody against HBcAg

Specifications:

Source : white rabbit

Appearance : liquid form（PBS containing biochemically inactive preservative）

Concentration: 1 mg/mL

Antigen: HBcAg, recombinant (E. coli)

Purification: affinity by Protein A

Usage : ELISA (0.1 to 10μg/mL), Western blot (0.1 to 10μg/mL)


Examples:

1. Sandwich ELISA to measure HBcAg
 

Immobilized IgG: anti-HBcAg antibody at 1μg/mL Detection IgG: anti-HBcAg antibody-HRP at 0.15μg/mL Antigen to measure: HBcAg (Code#: FNK-BCL-HBC-01)

2. Western blot
 

Sample: HBcAg Primary antibody: anti-HBcAg antibody (Code#: FNK-BCL-ABPC-01) at 1?g/mL Second antibody: anti-rabbit IgG-HRP (1/10000 dilution) Detection: ECL substrate