Fungal gDNA Kit (50 Preps)

Product#: BMG-GD2416-01
$90.00
Availability:
Ships in 1-2 Weeks

Fungal gDNA Kit (50 Preps)

Cat. No.: BMG-GD2416-01
Size: 50 Preps
 

Description: 

A Fungal gDNA Kit is a specialized tool designed for the extraction and purification of genomic DNA from fungal samples.

Applications: 
  • These kits are essential for isolating genomic DNA to study fungal genetics, gene function, and molecular mechanisms.
  • Extracted DNA can be used to detect and identify fungal pathogens in clinical or environmental samples using PCR or sequencing methods.
  • The kits enable studies on fungal genetics and gene function.
  • They allow detection of fungi in environmental or food samples.
  • Some rapid extraction methods are suitable for PCR screening of fungal mutants.
  • Certain kits can extract fungal DNA from infected plant material for studying plant-pathogen interactions.
  • Some methods are optimized for extracting DNA from fungal spores collected in traps.
  • The kits enable extraction of fungal DNA from complex environmental or clinical samples for microbiome sequencing.
  • Extracted DNA can be analyzed to detect antifungal resistance genes.
  • These kits allow detection of fastidious or unculturable fungi directly from clinical specimens.
Components:
  • Lysis buffers to break open fungal cell walls.
  • Enzymes for protein digestion.
  • Binding buffers to facilitate DNA adsorption.
  • Wash buffers to remove impurities.
  • Elution buffer to recover purified DNA.
  • Spin columns or magnetic beads for DNA purification.
Extraction Methods:
  • Specialized lysis techniques (enzymatic digestion, bead-beating).
  • Spin column-based purification with silica membrane technology.
Features:
  • Rapid extraction (typically <1 hour) from tough-to-lyse fungi.
  • Suitable for fresh and dried samples, including polysaccharide-rich tissues.
  • Processing capacity of 20-200 mg wet tissue or 30-50 mg dry tissue.
  • Effective removal of PCR inhibitors.
  • Typical yield of 5-30 μg purified DNA.
  • Elution volumes of 50-100 μL.
  • Suitable for various downstream applications (PCR, sequencing).
  • Options for manual or automated processing.
  • Available in different formats for various throughput needs.
  • All-inclusive kits with necessary reagents and buffers.
Reference:
Kumar M, Mugunthan M. Evaluation of three DNA extraction methods from fungal cultures. Med J Armed Forces India. 2018 Oct;74(4):333-336. doi: 10.1016/j.mjafi.2017.07.009. Epub 2017 Oct 5. PMID: 30449918; PMCID: PMC6224647.
Gudisa R, Harchand R, Rudramurthy SM. Nucleic-Acid-Based Molecular Fungal Diagnostics: A Way to a Better Future. Diagnostics (Basel). 2024 Feb 29;14(5):520. doi: 10.3390/diagnostics14050520. PMID: 38472992; PMCID: PMC10931343.




 

Satisfaction
Quality Rating
Value Rating
Style Rating
X