The EZgeneTM Viral DNA/RNA mini kit provides an easy and reliable method for isolating total viral DNA/RNA from plasma, serum, cell free body fluids, and cell culture supernatant. This procedure has been tested for isolating nucleic acids from Hepatitis A, Hepatitis C and HIV. The isolated DNA/RNA can be used for PCR, RT-PCR and other downstream applications.
Storage and Stability:
All other components can be stored at room temperature. All kit components are guaranteed for 1 year from the date of purchasing.
Kit Contents:
Catalog#
BMG-VR6511-00
BMG-VR6511-01
BMG-VR6511-02
Preps
4
50
250
Buffer LY
5 mL
30 mL
130 mL
L Solution
50 µL
120 µL
520 µL
RNA Wash Buffer *
1 mL
12 mL
50 mL
Buffer RB
5 mL
30 mL
130 mL
DEPC-Treated ddH2O
500 µL
10 mL
30 mL
Mini Columns
4
50
250
*Caution: Buffer LY and Buffer RB contain chaotropic salts, wear gloves and protection eyewear when handling these buffers.
Before Starting:
Prepare all components and get all necessary materials ready by examining this instruction booklet and become familiar with each steps.
Materials supplied by users:
Tabletop microcentrifuge
1.5 mL sterile RNAse free tubes.
100% ethanol
Important Notes:
Calculate and aliquot amount of Buffer LY to be used in a clean tube and add 1% volume of ß-mercaptoethanol to Buffer LY. Add 4 µL of L Solution per 1 mL of Buffer LY/ß-me. Mix well.
Prepare RNA Wash Buffer:
VR6511-00: Add 4 mL 100% ethanol to RNA Wash Buffer before use.
VR6511-01: Add 48 mL 100% ethanol to RNA Wash Buffer before use.
VR6511-02: Add 200 mL 100% ethanol to RNA Wash Buffer before use.
