This is a category page, not an orderable product page. Pick the concentration and fill your workflow needs and click View to reach its product page — the specifications, applications, and FAQ follow below.
Every item listed here is Sterile and calcium- and magnesium-free — the choice is concentration and fill. Catalog numbers are grouped under the product page they order from; click View once per group.
Cat. No.
Concentration
Size
FluxMPS™ linePBS, 1X (Phosphate Buffered Saline), Sterile1X · Ready to use · 2 catalog numbers · 500 ml · 1000 ml
Sterile 1X PBS is a balanced salt solution used for a wide range of applications (cell culture, washing cells, transporting cells or tissue, diluting cells for counting, and preparing other laboratory reagents). This PBS product is without calcium and magnesium for the purpose of rinsing cells before treating them with proteases for dissociation. We offer a variety of PBS formulations and customization for a range of cell cultures and laboratory applications.
ASTM Type 1 Ultrapure WaterTissue Culture GradeMolecular Biology Grade
Reading the fill-size row. The 10 Liter fill appears in the source catalog only against DCP-PBS10X_10 Liter. The 1X items are listed in 500 ml and 1000 ml, and the 20X item in 500 ml. Sizes are shown per catalog number in the table above rather than pooled, so nothing is implied for a configuration the source does not list.
Why It Matters
Six Reasons This Particular PBS Is Specified the Way It Is
Every point below is drawn from the source description and Feature list for this category.
water_drop
A balanced salt solution with a wide remit
Sterile 1X PBS is a balanced salt solution used for a wide range of applications: cell culture, washing cells, transporting cells or tissue, diluting cells for counting, and preparing other laboratory reagents.
block
Calcium- and magnesium-free on purpose
This PBS product is without calcium and magnesium for the purpose of rinsing cells before treating them with proteases for dissociation. The Feature list states CaCl2 (−) and MgCl2 (−) explicitly.
biotech
Nuclease- and protease-free
Protease (−), DNAse (−), RNAse (−), Endonuclease (−) — the four activities that most often show up as unexplained loss in downstream nucleic acid and protein work.
opacity
Built on ASTM Type 1 ultrapure water
Made with ASTM 0.05 µS/cm Type 1 Ultrapure H2O, deionized — the water grade the source specifies for this line.
science
One grade statement covering two disciplines
Tissue Culture & Molecular Biology grade, so a single stock buffer can serve both the culture hood and the bench without a second SKU.
tune
Formulations and customization on offer
The source states that a variety of PBS formulations and customization is offered for a range of cell cultures and laboratory applications. Contact support@diagnocine.com.
The distinction worth getting right
This is the calcium- and magnesium-free formulation. The source ties that choice to one specific step: rinsing cells before treating them with proteases for dissociation. If a protocol instead calls for calcium and magnesium present — for example a transport or reagent-preparation step — the item on this page is not that item, and a different formulation should be specified.
3
Sterile concentrations listed — 1X, 10X, 20X
0.05
µS/cm ASTM Type 1 ultrapure deionized water used
Selection Path
Four Questions, in Order, and You Are on the Right Product Page
The catalog above varies along one axis only — concentration and fill. These four checks get you to the right catalog number.
1
Formulation Is calcium- and magnesium-free the right buffer for this step?
Every item on this page is CaCl2 (−) and MgCl2 (−). The source ties that formulation to rinsing cells before protease treatment for dissociation. If the protocol needs calcium and magnesium present, stop here and ask about another formulation.
2
Format Ready-to-use 1X, or a concentrate?
The catalog lists a 1X presentation and two concentrates, 10X and 20X. The source states no dilution instruction and no working-strength figure, so confirm both on the individual product page before ordering.
3
Fill Which size, and is it listed for that concentration?
1X is listed in 500 ml and 1000 ml. 10X is listed in 500 ml, 1000 ml, and 10 Liter. 20X is listed in 500 ml. Each size carries its own catalog number, so order against the catalog number rather than the product name.
4
Custom Does a stock item actually fit?
The source states that a variety of PBS formulations and customization is offered for a range of cell cultures and laboratory applications. If none of the six catalog numbers matches the protocol, that is the route — support@diagnocine.com.
Configuration Reference
The Three Product Pages, Side by Side
Every value below is transferred from the source catalog exactly as published. Where the source states nothing, the cell says so rather than carrying an estimate.
Configuration
Concentration
Sterility
Ca / Mg
Sizes listed
Catalog numbers
PBS, 1X (Phosphate Buffered Saline), Sterile
1X
Sterile
cancel CaCl2 (−), MgCl2 (−)
500 ml · 1000 ml
DCP-PBS1X_500ml · DCP-PBS1X_1000ml
PBS, 10X (Phosphate Buffered Saline), Sterile
10X
Sterile
cancel CaCl2 (−), MgCl2 (−)
500 ml · 1000 ml · 10 Liter
DCP-PBS10X_500 ml · DCP-PBS10X_1000 ml · DCP-PBS10X_10 Liter
PBS, 20X (Phosphate Buffered Saline), Sterile
20X
Sterile
cancel CaCl2 (−), MgCl2 (−)
500 ml
DCP-PBS20X
Reading the water specification. The source states this line is made with “ASTM 0.05 µS/cm Type 1 Ultrapure H2O | Deionized”. For context, the published ASTM D1193 table sets the Type I ceiling at 0.056 µS/cm maximum electrical conductivity at 298 K, with a minimum electrical resistivity of 18 MΩ·cm; the source figure therefore sits inside the Type I limit rather than contradicting it. Consult the controlling published ASTM table for the full set of Type I constituent limits.
Applications
Where This PBS Belongs
Applications below are the ones named in the source description. The source assigns no application exclusively to a particular concentration, so none is invented here.
Stated for Sterile 1X PBS
Cell culture
Washing cells
Transporting cells or tissue
Diluting cells for counting
Preparing other laboratory reagents
Calcium- and magnesium-free rationale
Rinsing cells before treating them with proteases for dissociation
This is the single purpose the source gives for the calcium- and magnesium-free formulation. It names no other application that is exclusive to it.
Beyond the six catalog numbers
A variety of PBS formulations is offered for a range of cell cultures and laboratory applications
Customization is offered for a range of cell cultures and laboratory applications
The source states the offer but gives no list of available variables or limits, so none is stated here. Contact support@diagnocine.com with the target specification.
Shared Specifications
What the Source States for Every Item on This Page
Parameter
Stated value
Calcium chloride
CaCl2 (−)
Magnesium chloride
MgCl2 (−)
Protease
(−)
DNAse
(−)
RNAse
(−)
Endonuclease
(−)
Water used
ASTM 0.05 µS/cm Type 1 Ultrapure H2O | Deionized
Grade
Tissue Culture & Molecular Biology grade
Sterility
Sterile — stated in every product name on this catalog
Use policy
RUO* | R&D use only | Not for human therapeutic use or human consumption
Documentation. For a Certificate of Analysis, a specification sheet, or a custom formulation, contact support@diagnocine.com.
FAQ
Questions Buyers Ask Before They Pick a Concentration
The source describes Sterile 1X PBS as a balanced salt solution used for a wide range of applications: cell culture, washing cells, transporting cells or tissue, diluting cells for counting, and preparing other laboratory reagents.
The source states the product is without calcium and magnesium for the purpose of rinsing cells before treating them with proteases for dissociation. The Feature list records CaCl2 (-) and MgCl2 (-) explicitly.
All three are listed as Sterile and all three are calcium- and magnesium-free; the catalog difference is concentration and the fills offered. The source states no dilution instruction and no working-strength figure, so confirm both on the individual product page before ordering.
The source catalog lists 1X in 500 ml and 1000 ml, 10X in 500 ml, 1000 ml, and 10 Liter, and 20X in 500 ml. Note that the 1X product-page address contains a 10 Liter token, but no 10 Liter row is listed for the 1X item in the source catalog; confirm availability on the product page.
The Feature list states Protease (-), DNAse (-), RNAse (-), and Endonuclease (-), and gives the grade as Tissue Culture and Molecular Biology grade. The source states no numerical assay limits for those activities, so consult the Certificate of Analysis if a specification figure is required.
The source states this line uses ASTM 0.05 microsiemens per cm Type 1 ultrapure deionized water. For context, the published ASTM D1193 table sets Type I at a maximum electrical conductivity of 0.056 microsiemens per cm at 298 K and a minimum electrical resistivity of 18 megohm-cm, so the figure in the source sits inside the Type I limit rather than contradicting it.
The source states that a variety of PBS formulations and customization is offered for a range of cell cultures and laboratory applications. It does not list which variables can be changed or within what limits, so send the target specification to support@diagnocine.com. Note that all items on this page are Research Use Only, for R and D use only, and not for human therapeutic use or human consumption.
References
Supporting Literature and Standards
The source description carries no citation or reference list. The entries below are supporting literature and controlling standards, each verified against the primary record before it was written here; none is cited by the source itself.
Dulbecco R, Vogt M. Plaque formation and isolation of pure lines with poliomyelitis viruses. J Exp Med. 1954;99(2):167–182. doi:10.1084/jem.99.2.167
Eagle H. Amino acid metabolism in mammalian cell cultures. Science. 1959;130(3373):432–437. doi:10.1126/science.130.3373.432
ASTM International. ASTM D1193-06(2018), Standard Specification for Reagent Water. West Conshohocken, PA: ASTM International. Controlling table for the Type I water figures discussed above.
U.S. Food and Drug Administration. Bacteriological Analytical Manual (BAM), R19: Dulbecco's Phosphate-Buffered Saline (DPBS). Silver Spring, MD: FDA.
Thermo Fisher Scientific (Gibco). DPBS, no calcium, no magnesium — product technical information. Manufacturer technical reference corroborating that calcium- and magnesium-free formulations are used for rinsing chelators from the culture before cell dissociation.
