PBS, 20X (Phosphate Buffered Saline), Sterile
Cat. No. DCP-PBS20X
Size: 500ml & 1000ml
*Also available, PBS, 1X (Phosphate Buffered Saline), Sterile, 500ml/1000ml/10Liter
Product#: DCP-PBS1X >>> click here <<< and
PBS, 10X (Phosphate Buffered Saline), Sterile, 500ml/1000ml/10Liter
Product#: DCP-PBS10X >>> click here <<<
pH: pH 7.4 | Available other customization of pH, other customization of concentration (1X, 2X, 4X, 5X) | Please Inquire
Storage: Room Temperature; 4°C recommended for a long term storage
Shipping: Room temperature
Form & Appearance: Sterilized Liquid, Clear, Colorless
All Reagents were Sterilized by Filtering TWICE (2X) through 0.1 micron membrane filters and once through 0.04 micron membrane filter in a clean environment.
* Please note that salt might precipitate.
Product Description:
All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced salt solution (BSS). All the physiological salt solutions have been derived from the salt solution originally described by Sydney Ringer (1885). The first balanced salt solution to be developed specifically for supporting the metabolism of mammalian cells was Tyrode's solution. Since then many modifications have been done to obtain better buffering salt solutions and to prevent calcium precipitation.
The function of a salt solution is:
- To maintain the medium within physiological pH range.
- To maintain intracellular and extra cellular osmotic balance.
- When modified with a carbohydrate such as glucose, it serves as an energy source for cell metabolism.
This is 20X Phosphate Buffered Saline, pH 7.4. It does not contain calcium and magnesium and hence commonly used for tissue disaggregation and monolayer dispersal since presence of calcium and magnesium ions may hinder the trypsin activity. It is also used for routine immunohistochemical testing and as a general purpose solution for washing cells in various hematological and molecular biology procedures.
Feature:
- CaCl2 (-), MgCl2 (-), Protease (-), DNAse (-), RNAse (-), Endonuclease (-)
- Used ASTM 0.05 µS/cm Type 1 Ultrapure H2O | Deionized
- Tissue Culture & Molecular Biology grade
- RUO* | R&D use only | Not for human therapeutic use or human consumption
*RUO – Research Use Only
Composition:
| Ingredients |
mg/L |
| INORGANIC SALTS |
| Disodium hydrogen phosphate anhydrous |
14520.000 |
| Potassium dihydrogen phosphate |
4200.000 |
| Sodium chloride |
18000.000 |
Quality Control:
- Appearance: Colorless, clear solution.
- pH at 20X: 7.4
- pH at 1X: 7.00 - 7.40
- Sterility: No bacterial or fungal growth is observed after 14 days of incubation,as per USP specification.
- Toxicity Test: Passes
- Endotoxin Content: NMT 1EU/ml
Storage and Shelf Life:
- Store at 15- 30°C away from bright light.
- Shelf life is 24 months.
- Use before expiry date given on the product label.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA
