PBS, 20X (Phosphate Buffered Saline), Sterile

FluxMPS™ PBS, 20X (Phosphate Buffered Saline), Sterile

An MPS-grade, calcium- and magnesium-free 20X Phosphate Buffered Saline concentrate at pH 7.4, quadruple-stage filtered (0.1 µm twice, 0.04 µm twice) in a clean environment for ultra-low particulate and endotoxin control. Formulated with ASTM Type 1 ultrapure, deionized water for microchannel-safe performance in organ-on-a-chip (OoC), tissue culture, and molecular biology workflows.

• Quadruple-stage filtration: 0.1 µm membrane filtration performed twice and 0.04 µm membrane filtration performed twice, in a clean environment
• Endotoxin content NMT 1EU/mL; no bacterial or fungal growth after 14 days incubation per USP specification
• pH 7.4 at 20X concentration; pH 7.00-7.40 when diluted to 1X
• Calcium- and magnesium-free formulation (CaCl₂ (-), MgCl₂ (-)) — ideal where trypsin activity must not be hindered
• Manufactured with ASTM Type 1 ultrapure, deionized water (0.05 µS/cm)
• Sterilized liquid, clear and colorless; Tissue Culture & Molecular Biology grade
• Customizable pH and concentration (1X, 2X, 4X, 5X) available on request — contact support@diagnocine.com

Engineered where standard buffers fail

Balanced salt solutions trace back to Sydney Ringer's original 1885 physiological salt solution and Tyrode's solution, developed to support mammalian cell metabolism, maintain physiological pH, and preserve intracellular and extracellular osmotic balance. Conventional 0.22 µm-filtered buffers can carry subvisible particulates, salt precipitation risk, and inconsistent pH/ionic conditions into sensitive microchannels. FluxMPS™ PBS, 20X is quadruple-stage filtered and manufactured under controlled, documented conditions to reduce these failure modes.

Quadruple-stage filtration system

All reagents in this product are sterilized by filtering through 0.1 micron membrane filters twice and through 0.04 micron membrane filters twice, in a clean environment, layering coarse and fine membrane passes for progressively lower particulate carryover.

1. 1

   0.1 µm Pre-filtration I

   First 0.1 micron membrane pass removes larger particulates and aggregates ahead of subsequent stages.

2. 2

   0.04 µm Pre-filtration II

   First 0.04 micron membrane pass retains finer particulates, reducing bioburden ahead of final sterile filtration.

3. 3

   0.1 µm Sterile-filtration I

   Second 0.1 micron membrane pass provides redundant sterile-filtration coverage in the clean environment.

4. 4

   0.04 µm Sterile-filtration II — Final Polish

   Second 0.04 micron membrane pass delivers the final polish, completing the clean-environment sterilization process.

Where FluxMPS™ PBS, 20X performs

Free of calcium and magnesium, this 20X concentrate is used for tissue disaggregation and monolayer dispersal, since calcium and magnesium ions may hinder trypsin activity, as well as for routine immunohistochemical testing and general-purpose washing of cells in hematological and molecular biology procedures.

Automated Bioreactors & Robotics

An optional 10 nm (0.01 µm) ultra-filtered variant is available for automated bioreactor and robotic liquid-handling platforms where valve and sensor protection is critical.

• Total Particulate Exclusion for automated fluid paths
• Valve & Sensor Protection in closed-loop perfusion systems
• Extended Perfusion Stability across long-duration runs

Inquiry Required: the 0.01 µm ultra-filtered grade is produced to order; contact support@diagnocine.com to request this configuration.

Detailed specifications

All values below are as declared for this product.

Full composition (mg/L)

Inorganic salt composition of the 20X concentrate, released per lot. Please note that salt might precipitate; gentle warming and mixing typically resolves this prior to use.

Manufacturing & compliance

Produced under ISO 13485-certified and CE-approved facilities, with final packaging, quality assurance, and testing completed at the DiagnoCine R&D and Quality Testing Center.

How DCP-PBS20X compares

A qualitative comparison against a conventional single-pass filtered buffer and a standard laboratory alternative.

Frequently asked questions

Common questions about DCP-PBS20X.

Supporting literature

Curated references on balanced salt solutions, buffer chemistry, and microfluidic/organ-on-a-chip applications.

1. Ringer S. Concerning the influence exerted by each of the constituents of the blood on the contraction of the ventricle. J Physiol. 1883. doi:10.1113/jphysiol.1883.sp000120
2. Tyrode MV. The mode of action of some purgative salts. Arch Int Pharmacodyn Ther. 1910. doi:10.1002/cbf.1085
3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760-772. doi:10.1038/nbt.2989
4. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662-1668. doi:10.1126/science.1188302
5. Good NE, et al. Hydrogen ion buffers for biological research. Biochemistry. 1966;5:467-477. doi:10.1021/bi00866a011
6. Freshney RI. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications. Wiley. doi:10.1002/9780470649367
7. Low LA, Tagle DA. Organs-on-chips: progress, challenges, and future directions. Exp Biol Med. 2017;242:1573-1578. doi:10.1177/1535370217700523
8. Williams DF. Endotoxin testing and its role in biocompatibility. Biomaterials. 2011. doi:10.1016/j.biomaterials.2011.01.019
9. van Meer BJ, et al. Small molecule absorption by PDMS in the context of drug response bioassays. Biochem Biophys Res Commun. 2017. doi:10.1016/j.bbrc.2017.01.164