Dulbecco's PBS (D-PBS) — Configuration Selection Guide
This is a category page, not an orderable product page. Pick the configuration your protocol needs and click View to reach its product page — the specifications, applications, and FAQ follow below.
| Cat. No. | Name | Ca²? / Mg²? | Sterile | FluxMPS™ | Size | Product Page |
|---|---|---|---|---|---|---|
| FluxMPS™ lineDCP-DPBS1X_500 mlWith Ca/Mg · Sterile · 1X | Dulbecco's PBS with Ca2+and Mg2+ (D-PBS), Sterile, 1X | + | + | + | 500 ml | Viewarrow_forward |
| FluxMPS™ lineDCP-DPBS1X_1000 mlWith Ca/Mg · Sterile · 1X | Dulbecco's PBS with Ca2+and Mg2+ (D-PBS), Sterile, 1X | + | + | + | 1000 ml | Viewarrow_forward |
| FluxMPS™ lineDCP-DPBS1X_10 literWith Ca/Mg · Sterile · 1X | Dulbecco's PBS with Ca2+and Mg2+ (D-PBS), Sterile, 1X | + | + | + | 10 liter | Viewarrow_forward |
| FluxMPS™ lineDCP-DPBSWOCAMG1X_500mlWithout Ca/Mg · Sterile | Dulbecco's PBS without Ca2+and Mg2+ (D-PBS), Sterile | − | + | + | 500 ml | Viewarrow_forward |
| FluxMPS™ lineDCP-DPBSWOCAMG1X_1000mlWithout Ca/Mg · Sterile | Dulbecco's PBS without Ca2+and Mg2+ (D-PBS), Sterile | − | + | + | 1000 ml | Viewarrow_forward |
| FluxMPS™ lineDCP-DPBSWOCAMG1X_10 LiterWithout Ca/Mg · Sterile | Dulbecco's PBS without Ca2+and Mg2+ (D-PBS), Sterile | − | + | + | 10 liter | Viewarrow_forward |
| FluxMPS™ lineDCP-DPBS10X_500 mlWith Ca/Mg · Non-Sterile · 10X | Dulbecco's PBS with Ca2+and Mg2+ (D-PBS), Non-Sterile, 10X | + | − | + | 500 ml | Viewarrow_forward |
| FluxMPS™ lineDCP-DPBS10X_1000 mlWith Ca/Mg · Non-Sterile · 10X | Dulbecco's PBS with Ca2+and Mg2+ (D-PBS), Non-Sterile, 10X | + | − | + | 1000 ml | Viewarrow_forward |
| FluxMPS™ lineDCP-DPBS10X_10 LitersWith Ca/Mg · Non-Sterile · 10X | Dulbecco's PBS with Ca2+and Mg2+ (D-PBS), Non-Sterile, 10X | + | − | + | 10 liter | Viewarrow_forward |
| FluxMPS™ lineDCP-DPBSWOCAMG10X_500 mlWithout Ca/Mg · Non-Sterile · 10X | Dulbecco's PBS without Ca2+and Mg2+ (D-PBS), Non-Sterile, 10X | − | − | + | 500 ml | Viewarrow_forward |
| FluxMPS™ lineDCP-DPBSWOCAMG10X_1000 mlWithout Ca/Mg · Non-Sterile · 10X | Dulbecco's PBS without Ca2+and Mg2+ (D-PBS), Non-Sterile, 10X | − | − | + | 1000 ml | Viewarrow_forward |
| FluxMPS™ lineDCP-DPBSWOCAMG10X_10 LitersWithout Ca/Mg · Non-Sterile · 10X | Dulbecco's PBS without Ca2+and Mg2+ (D-PBS), Non-Sterile, 10X | − | − | + | 10 liter | Viewarrow_forward |
| Separate supply lineFNK-87-972PBS(-)Not a FluxMPS™ item. Formulation attributes are not stated in the source description — see the product page. | Dulbecco's Phosphate-Buffered Saline, PBS(-) | n/s | n/s | − | 1000 ml | Viewarrow_forward |
| Separate supply lineFNK-87-948PBS(-)Not a FluxMPS™ item. Formulation attributes are not stated in the source description — see the product page. | Dulbecco's Phosphate-Buffered Saline, PBS(-) | n/s | n/s | − | 12 X 1000 ml | Viewarrow_forward |
Not sure which configuration? Compare the configurations side by side · See applications by configuration · Read the FAQ
What Dulbecco's PBS Is, in the Source's Own Words
Dulbecco's phosphate-buffered saline (DPBS) is a balanced salt solution designed for multiple cell culture applications due to its osmotic balance property similar to the natural condition of mammalian systems. D-PBS is generally used to provide the appropriate milieu to sustain the physiological and structural integrity (i.e., tonicity/viability) of cells in vitro briefly. Balanced Salts support viable conditions for cells only during experimental conditions outside of their regular growth environment. When treated with D-PBS, the cell maintains osmotic balance and physiological pH, and D-PBS protects the cells from acute fluctuations.
A wide range of uses include (a) general cell culture, (b) washing adhesion cells before dissociation, (c) transporting cells or tissue samples, (d) diluting cells for counting, and (e) preparing reagents for providing inorganic salts essential for basic cell metabolism. D-PBS is widely used because its buffering capacity is effective at in vivo neutral pH in various applications. When included with calcium and magnesium, trypsinization / cell dissociation is less effective, but such inclusion is useful for transport media or for reagent preparation. In addition, D-PBS is generally used as a diluent or as an inorganic base for standard media preparation. Deterioration of the D-PBS may be recognized by precipitate or particulate matter throughout the solution, cloudy appearance, color change, and/or pH change.
- Protease (−), DNAse (−), RNAse (−), Endonuclease (−)
- Used ASTM 0.05 µS/cm Type 1 Ultrapure H2O | Deionized
- Tissue Culture & Molecular Biology grade
- Supplied with Ca2+ and Mg2+ and without Ca2+ and Mg2+
- Supplied as 1X and as 10X, in Sterile and Non-Sterile configurations
- Fill sizes on the D-PBS line: 500 ml, 1000 ml, and 10 liter
- A separately supplied Dulbecco's Phosphate-Buffered Saline, PBS(-) is listed in 1000 ml and 12 X 1000 ml
- Catalog numbers on this page14
- Product pages linked6
- Ca2+ / Mg2+ optionsWith / Without
- Concentrations1X / 10X
- Sterility optionsSterile / Non-Sterile
- Fill sizes — D-PBS line500 ml / 1000 ml / 10 liter
- Fill sizes — PBS(-) line1000 ml / 12 X 1000 ml
- Water used — D-PBS lineASTM Type 1, 0.05 µS/cm
- Nuclease / protease status — D-PBS lineProtease, DNAse, RNAse, Endonuclease (−)
- Grade — D-PBS lineTissue Culture & Molecular Biology
DCP-. The source lists FNK-87-972 and FNK-87-948 separately, and states no corresponding Features for them; those fields are therefore left blank rather than inferred. Confirm their specifications on their own product pages.Six Things D-PBS Is Doing While Your Cells Are Off the Incubator
Every point below is drawn from the source description for this category.
Osmotic balance close to the mammalian condition
D-PBS is a balanced salt solution designed for multiple cell culture applications because its osmotic balance property is similar to the natural condition of mammalian systems.
Buffering at in vivo neutral pH
D-PBS is widely used because its buffering capacity is effective at in vivo neutral pH across a range of applications. Treated cells maintain osmotic balance and physiological pH.
Protection from acute fluctuations
D-PBS protects cells from acute fluctuations while they are held outside their regular growth environment.
A short-term holding solution, by design
Balanced salts support viable conditions for cells only during experimental conditions outside of their regular growth environment. D-PBS sustains physiological and structural integrity — tonicity and viability — briefly.
Calcium and magnesium change what the buffer is for
When included with calcium and magnesium, trypsinization / cell dissociation is less effective — but that inclusion is useful for transport media or for reagent preparation.
Deterioration is visible before it is measurable
Deterioration of the D-PBS may be recognized by precipitate or particulate matter throughout the solution, cloudy appearance, color change, and/or pH change.
The one decision that changes the result
Both calcium- and magnesium-containing and calcium- and magnesium-free D-PBS are stocked on this page, and they are not interchangeable. The source is explicit: with calcium and magnesium present, trypsinization and cell dissociation are less effective, while that same formulation is the useful one for transport media and reagent preparation. Choose the formulation by the step it has to support, not by what is on the shelf.
Four Questions, in Order, and You Are on the Right Product Page
The catalog above splits along exactly four axes. Answer them in this order — each one narrows the list.
-
1
Formulation With or without Ca2+ and Mg2+?
If the next step is washing adhesion cells before dissociation, the source points away from calcium and magnesium: their inclusion makes trypsinization / cell dissociation less effective. If the buffer is going to be a transport medium or a base for reagent preparation, the source calls that inclusion useful.
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2
Format 1X or 10X?
The catalog lists both a 1X presentation and a 10X presentation for the calcium- and magnesium-containing and the calcium- and magnesium-free formulations. Confirm the working strength and the dilution instruction on the individual product page before ordering.
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3
Sterility Sterile or Non-Sterile?
On this catalog the 1X items are named Sterile and the 10X items are named Non-Sterile. If the solution will contact a live culture without a further sterilizing step, that naming is the first thing to check.
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4
Fill Which size?
Every D-PBS configuration is listed in 500 ml, 1000 ml, and 10 liter. The separately supplied Dulbecco's Phosphate-Buffered Saline, PBS(-) is listed in 1000 ml and 12 X 1000 ml. Each size carries its own catalog number, so order against the catalog number, not the product name.
The Six Product Pages, Side by Side
Every value below is transferred from the source catalog exactly as published. Blank cells are blank because the source states nothing for them — they are not estimates.
| Configuration | Ca2+ / Mg2+ | Concentration | Sterility | Sizes listed | Supply line |
|---|---|---|---|---|---|
| Dulbecco's PBS with Ca2+and Mg2+ (D-PBS), Sterile, 1X | check_circle With | 1X | Sterile | 500 ml · 1000 ml · 10 liter | FluxMPS™ |
| Dulbecco's PBS without Ca2+and Mg2+ (D-PBS), Sterile | cancel Without | Catalog numbers carry a 1X token; the product name states none | Sterile | 500 ml · 1000 ml · 10 liter | FluxMPS™ |
| Dulbecco's PBS with Ca2+and Mg2+ (D-PBS), Non-Sterile, 10X | check_circle With | 10X | Non-Sterile | 500 ml · 1000 ml · 10 liter | FluxMPS™ |
| Dulbecco's PBS without Ca2+and Mg2+ (D-PBS), Non-Sterile, 10X | cancel Without | 10X | Non-Sterile | 500 ml · 1000 ml · 10 liter | FluxMPS™ |
| Dulbecco's Phosphate-Buffered Saline, PBS(-) — FNK-87-972 | Not stated in source | Not stated in source | Not stated in source | 1000 ml | Separate supply line — not FluxMPS™ |
| Dulbecco's Phosphate-Buffered Saline, PBS(-) — FNK-87-948 | Not stated in source | Not stated in source | Not stated in source | 12 X 1000 ml | Separate supply line — not FluxMPS™ |
Where Each Formulation Belongs
Applications below are the ones named in the source description, split by the formulation the source ties them to.
- General cell culture
- Washing adhesion cells before dissociation
- Transporting cells or tissue samples
- Diluting cells for counting
- Preparing reagents for providing inorganic salts essential for basic cell metabolism
- Use as a diluent, or as an inorganic base for standard media preparation
- Transport media
- Reagent preparation
- Washing adhesion cells before dissociation
- Workflows where trypsinization / cell dissociation efficiency matters
What the Source States for the D-PBS Line
| Parameter | Stated value |
|---|---|
| Protease | (−) |
| DNAse | (−) |
| RNAse | (−) |
| Endonuclease | (−) |
| Water used | ASTM 0.05 µS/cm Type 1 Ultrapure H2O | Deionized |
| Grade | Tissue Culture & Molecular Biology grade |
| Buffering | Effective at in vivo neutral pH |
| Intended holding time | Brief — supports viable conditions only during experimental conditions outside the regular growth environment |
Questions Buyers Ask Before They Pick a Configuration
The source lists general cell culture, washing adhesion cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents that provide inorganic salts essential for basic cell metabolism. It is also used as a diluent and as an inorganic base for standard media preparation.
The source states that when calcium and magnesium are included, trypsinization and cell dissociation are less effective, but that the same inclusion is useful for transport media or for reagent preparation. If a dissociation step follows, the calcium- and magnesium-free configuration is the one the source points to.
The source describes D-PBS as sustaining the physiological and structural integrity of cells in vitro briefly, and states that balanced salts support viable conditions only during experimental conditions outside of their regular growth environment. It is not a growth medium. The source states no maximum holding time, so confirm the handling window on the product page.
Both are listed for the calcium- and magnesium-containing and the calcium- and magnesium-free formulations. In this catalog the 1X items are named Sterile and the 10X items are named Non-Sterile. The source gives no dilution instruction and no working-strength figure, so confirm both on the individual product page before ordering.
The source names four signs: precipitate or particulate matter throughout the solution, cloudy appearance, color change, and pH change.
The source states the D-PBS line uses ASTM 0.05 microsiemens per cm Type 1 ultrapure deionized water. For context, the published ASTM D1193 table sets Type I at a maximum electrical conductivity of 0.056 microsiemens per cm at 298 K and a minimum electrical resistivity of 18 megohm-cm, so the figure in the source sits inside the Type I limit rather than contradicting it.
They are a separate supply line and are not FluxMPS items. The source description lists them apart from the DCP catalog numbers and states none of the D-PBS Features for them, so the water grade, the nuclease and protease statements, and the grade designation given on this page do not apply to them. Their formulation attributes are marked as not stated in the catalog above; confirm them on their own product pages.
Supporting Literature and Standards
The source description carries no citation or reference list. The entries below are supporting literature and controlling standards, each verified against the primary record before it was written here; none is cited by the source itself.
- Dulbecco R, Vogt M. Plaque formation and isolation of pure lines with poliomyelitis viruses. J Exp Med. 1954;99(2):167–182. doi:10.1084/jem.99.2.167
- Eagle H. Amino acid metabolism in mammalian cell cultures. Science. 1959;130(3373):432–437. doi:10.1126/science.130.3373.432
- ASTM International. ASTM D1193-06(2018), Standard Specification for Reagent Water. West Conshohocken, PA: ASTM International. Controlling table for the Type I water figures discussed above.
- U.S. Food and Drug Administration. Bacteriological Analytical Manual (BAM), R19: Dulbecco's Phosphate-Buffered Saline (DPBS). Silver Spring, MD: FDA.
- Thermo Fisher Scientific (Gibco). DPBS, no calcium, no magnesium — product technical information. Manufacturer technical reference corroborating that calcium- and magnesium-free formulations are used for rinsing chelators from the culture before cell dissociation.
