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Human clusterin,CLU ELISA Kit
Product#: CS-CSB-E09121h
$933.60
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Human ELISA Kit · Cardiovascular
Human clusterin,CLU ELISA Kit
Sandwich quantitative immunoassay for clusterin in human serum, plasma, cell culture supernates, saliva, urine available in multiple catalog sizes:
Trial 24T 96T
Note: Please send inquiries regarding Trial 24T orders to support@diagnocine.com.
Detection Range
3.12-200 ng/mL
Sensitivity
0.78 ng/mL
Assay Time
1-5 hours
Sample Volume
50-100 μL
Product specifications
| Target name | clusterin |
| Uniprot No. | P10909 |
| Species | Homo sapiens (Human) |
| Sample types | serum, plasma, cell culture supernates, saliva, urine |
| Detection range | 3.12 ng/mL-200 ng/mL |
| Sensitivity | 0.78 ng/mL |
| Assay time | 1-5h |
| Sample loading volume | 50-100μL |
| Detection wavelength | 450 nm |
| Assay principle | Sandwich (Quantitative) |
| Data analysis | Standard curve + Curve Expert software |
| Research area | Cardiovascular |
| Storage condition | 2-8°C (see protocol for full details) |
| Shipping condition | 4 °C |
| Shelf life | 6 months |
Lead Time
10 business days
Processing + 3-5 days shipping
Availability
In Stock : USA
Worldwide shipping available
Assay principle
Sample prep
Antibody capture
450 nm detection
Standard curve
Quantification
Antibody capture
450 nm detection
Standard curve
Quantification
In this sandwich ELISA, clusterin in the sample is captured between a pre-coated capture antibody and a detection antibody. Signal intensity is proportional to analyte concentration. Quantification uses a standard curve fitted with Curve Expert software, covering 3.12 ng/mL-200 ng/mL with a minimum detectable dose of 0.78 ng/mL.
For research use only (RUO). Not intended for diagnostic or therapeutic purposes. Validated in human serum, plasma, cell culture supernates, saliva, urine matrices only.
Description
This Human CLU ELISA Kit was designed for the quantitative measurement of Human CLU protein in serum, plasma, cell culture supernates, saliva, urine. It is a Sandwich ELISA kit, its detection range is 3.12 ng/mL-200 ng/mL and the sensitivity is 0.78 ng/mL.
Alternative Names
40 ELISA Kit; AAG 4 ELISA Kit; AAG4 ELISA Kit; Aging associated protein 4 ELISA Kit; Aging-associated gene 4 protein ELISA Kit; AI893575 ELISA Kit; APO J ELISA Kit; Apo-J ELISA Kit; APOJ ELISA Kit; ApoJalpha ELISA Kit; ApoJbeta ELISA Kit; Apolipoprotein J ELISA Kit; ApolipoproteinJ ELISA Kit; CLI ELISA Kit; CLU ELISA Kit; CLU1 ELISA Kit; CLU2 ELISA Kit; CLUS_HUMAN ELISA Kit; Clusterin alpha chain ELISA Kit; Clusterin ELISA Kit; Clusterin beta chain ELISA Kit; Complement associated protein SP 40 40 ELISA Kit; Complement associated protein SP 40 ELISA Kit; Complement associated protein SP40 ELISA Kit; Complement cytolysis inhibitor a chain ELISA Kit; Complement cytolysis inhibitor ELISA Kit; Complement cytolysis inhibitor b chain ELISA Kit; Complement lysis inhibitor ELISA Kit; Complement-associated protein SP-40 ELISA Kit; D14Ucla3 ELISA Kit; Dimeric acid glycoprotein ELISA Kit; Glycoprotein 80 ELISA Kit; Glycoprotein III ELISA Kit; GP80 ELISA Kit; Ku70-binding protein 1 ELISA Kit; KUB 1 ELISA Kit; KUB1 ELISA Kit; MGC24903 ELISA Kit; NA1/NA2 ELISA Kit; RATTRPM2B ELISA Kit; SGP 2 ELISA Kit; SGP2 ELISA Kit; SP 40 ELISA Kit; SP40 ELISA Kit; Sugp-2 ELISA Kit; Sulfated glycoprotein 2 ELISA Kit; Testosterone repressed prostate message 2 ELISA Kit; Testosterone-repressed prostate message 2 ELISA Kit; TRPM 2 ELISA Kit; TRPM-2 ELISA Kit; TRPM2 ELISA Kit; TRPM2B ELISA Kit; Trpmb ELISA Kit
Function
Functions as extracellular chaperone that prevents aggregation of non native proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Protects cells against apoptosis and against cytolysis by complement. Intracellular forms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. A mitochondrial form suppresses BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation. An intracellular form suppresses stress-induced apoptosis by stabilizing mitochondrial membrane integrity through interaction with HSPA5. Secreted form does not affect caspase or BAX-mediated intrinsic apoptosis and TNF-induced NF-kappa-B-activity. Secreted form act as an important modulator during neuronal differentiation through interaction with STMN3. Plays a role in the clearance of immune complexes that arise during cell injury.; Does not affect caspase or BAX-mediated intrinsic apoptosis and TNF-induced NF-kappa-B-activity.; Does not affect caspase or BAX-mediated intrinsic apoptosis and TNF-induced NF-kappa-B-activity. Promotes cell death through interaction with BCL2L1 that releases and activates BAX.
Gene References into Functions
- Downregulation of apoE and apoJ in CSF strongly suggests a critical role of lipid metabolism in the development and progression of Moyamoya disease. PMID: 28843803
- Data suggest that the potential of clusterin and glutathione synthetase (GSH-S) as platelet biomarkers for early detection of colorectal cancer (CRC) could improve existing screening modalities in clinical application. PMID: 28849249
- Our study may help to further elucidate the development and progression of non-small cell lung cancer , also it may contribute to the research of therapies targeting sCLU. PMID: 28954633
- data confirm that the association of psoriatic disease with some comorbidities, especially metabolic and cardiovascular disease, might support the correlation with increased circulating Clu. PMID: 28958138
- A total of 249 epileptic patients and 289 healthy controls were included in this study. Three Clusterin single nucleotide polymorphisms (SNPs: rs11136000, rs9314349, and rs9331949) were selected and genotyped with the SNaPshot assay. Our study failed to detect an association between Clusterin polymorphisms (rs11136000, rs9314349, and rs9331949) and epilepsy in a Han Chinese population. PMID: 28972394
- we confirm that a PEX/Alzheimer's Disease associated risk variant, rs2279590, resides within an enhancer element and regulates the expression of three candidate genes CLU, PTK2B and EPHX2, which were previously known to be modulators in the progression of Alzheimer's Disease. PMID: 28973302
- Serum ApoJ levels are closely correlated with the magnitude of insulin resistance. PMID: 28986164
- This study showed that the 1- and 2-year follow-up, generalized estimating equation analyses of Alzheimer's disease patients revealed that high levels of plasma clusterin at baseline were associated with a significantly larger decrease in Mini-Mental State Examination compared with low levels of plasma clusterin. PMID: 29169407
- plasma level decreases in healthy but not in asthmatic pregnancy and correlates directly with lung function PMID: 29200898
- Thus, we suggest that a combination of PI3K/AKT/mTOR inhibitors with V-ATPase inhibitors might be an effective approach for NSCLC treatment. PMID: 29253572
- Our study showed low clusterin immunostaining in colorectal carcinoma with lack of association with prognostic indicators PMID: 29279586
- plasma clusterin was associated with lower volume of the entorhinal cortex, an area that atrophies early in AD. Clusterin could be informative as part of a multi-component preclinical marker for AD PMID: 29324756
- that the Clusterin rs11136000 polymorphism C allele is associated with AD susceptibility PMID: 29396813
- clusterin promotes growth and invasion in renal cell carcinoma cells in vitro and in vivo through upregulation of S100A4 PMID: 29400663
- The results showed that secreted CLU was overexpressed in three hepatocellular carcinoma cell lines. The downregulation of CLU by CLU shRNA synergistically increased Sorafenib sensitivity in the Bel7402 and SMMC7721 cells, and potentiated Sorafenib induced cell apoptosis. PMID: 29436591
- Meta analysis validated the Alzheimer disease protective association for CLU (rs11136000) variants. PMID: 29504051
- The VDR/MEG3/Clusterin signaling pathway may serve as potential therapeutic targets and prognosis biomarkers for colorectal cancer patients in future. PMID: 29628342
- miR-195 improved the sensitivity of resistant prostate cancer cells to docetaxel by suppressing CLU. PMID: 29665645
- The plasma levels of septin-9 and clusterin in ovarian cancer patients were abnormally elevated, which might be used as potential candidates of peripheral blood tumor biomarkers for early diagnosis of EOC and septin-9 might be related to distal metastases of EOC. PMID: 29970704
++ 31 more research findings link this target to additional biology. Showing the 19 most recent — the full set appears on the complete datasheet.
Tissue Specificity
Detected in blood plasma, cerebrospinal fluid, milk, seminal plasma and colon mucosa. Detected in the germinal center of colon lymphoid nodules and in colon parasympathetic ganglia of the Auerbach plexus (at protein level). Ubiquitous. Detected in brain,
Subcellular Location
[Isoform 1]: Secreted.; [Isoform 4]: Cytoplasm.; [Isoform 6]: Cytoplasm.; Nucleus. Cytoplasm. Mitochondrion membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytosol. Microsome. Endoplasmic reticulum. Mitochondrion. Mitochondrion membrane. Cytoplasm, perinuclear region. Cytoplasmic vesicle, secretory vesicle, chromaffin granule.
Protein Families
Clusterin family
Database Links
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of human CLU in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 90 | ||||
| Range % | 85-96 | |||||
| 1:2000 | Average % | 97 | ||||
| Range % | 91-104 | |||||
| 1:4000 | Average % | 92 | ||||
| Range % | 85-100 | |||||
| 1:8000 | Average % | 93 | ||||
| Range % | 83-99 | |||||
Recovery
| The recovery of human CLU spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 92-106 | ||||
| EDTA plasma (n=4) | 95 | 90-99 | ||||
Peer-reviewed citations
++ 1 more peer-reviewed citations feature this kit. Showing the 1 most recent — browse every publication on the full product page.
